An hCMEC monolayer was formed in the flow chamber slides (ibidi) as described above. Then, two groups of cancer cells were marked with two different colors of cell trackers CytoTrace™ Green CMFDA (Thermo Fisher Scientific) and PKH26 Red Fluorescent Cell Linker (Sigma-Aldrich, Saint Louis, MA, USA) following the manufacturer’s instructions. In total, 100,000 cells from each group were mixed and then infused into one slide under 1 dyn/cm2 shear stress for 15 min. The number of adhered cells was counted.
Flow chamber slides
Manufactured by Ibidi
Flow chamber slides are specialized laboratory equipment used for various cellular and molecular biology applications. They provide a controlled microenvironment for the observation and analysis of cells, fluids, or other biological samples. The slides feature a sealed chamber design that allows for the precise control and manipulation of fluid flow, enabling researchers to study dynamic cellular processes, cell-cell interactions, and the effects of external stimuli on cells.
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Lab products found in correlation
2 protocols using flow chamber slides
1
Cancer Cell Adhesion Assay
2
Tumor Cell Adhesion to Endothelium
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The hCMEC monolayer was formed in the flow chamber slides (ibidi). A total of 300,000 cancer cells were marked with cell tracker CytoTrace™ Green CMFDA (Thermo Fisher Scientific) following the manufacturer’s instructions and then added into the slides. The slides were then incubated at 37 °C for 30 min to allow cancer cells to interact with the endothelium. DMEM full medium was perfused through the slide, creating certain level of shear stress for 15 min. The number of adhered cancer cells was counted.
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