Architect i2000sr immunoassay analyzer

Demographic and clinical characteristics and laboratory findings were collected on the day of renal biopsy and comprised information about age, gender, body mass index, and hematological, biochemical, and immunological test results. Serum HE4 level was measured using Architect HE4 kits and an Abbott ARCHITECT i2000SR Immunoassay Analyzer (Abbott Laboratories, Abbott Park, IL, USA) according to the manufacturer’s instructions. Briefly, a two-step immunoassay involving the chemiluminescent microparticle immunoassay technology with flexible assay protocols (Chemiflex) was used.

With the participant in the sitting position, blood samples were collected from the antecubital vein using 23-G needles [11 ]. The serum samples were separated by centrifugation (1500×g) at 4 °C for 10 min and were stored at − 80 °C until analysis [11 ]. A LABOSPECT 008 automatic analyzer (Hitachi, Tokyo, Japan) was used to measure serum albumin concentrations using the modified bromocresol purple method, and serum transthyretin and transferrin concentrations using turbidimetric immunoassays. Intra- and inter-assay CV were 0.5–0.7 and 1.5% for albumin, 1.3–1.8% and 0.6–1.1% for transthyretin, and 0.8–1.2% and 1.3–1.4% for transferrin, respectively. A chemiluminescent microparticle immunoassay on an Abbott ARCHITECT i2000SR Immunoassay Analyzer (Abbott Laboratories, Abbott Park, IL, USA) was used to analyze serum concentrations of free 3,5,3′-triiodothyronine (FT₃), free thyroxine (FT₄), and thyrotropin (TSH). Intra- and inter-assay CV were 5.1–5.7% and 0.5–2.8% for FT₃, 3.6–4.5 and 4.1% for FT₄, and 2.3–4.4% and 2.8–4.1% for TSH, respectively. Serum BTR was measured by the enzymatic method using JCA-BM6050 automatic analyzer (JOEL, Tokyo, Japan) at LSI Medience Co. (Tokyo, Japan). Intra- and inter-assay CV of BTR were 0.2–0.3% and 0.7–1.0%, respectively.

With the participants sitting down, blood samples were collected after an antecubital vein is punctured using 23-G needles. Serum samples were separated by centrifugation (1500×g) at 4 °C for 10 min and were stored at − 80 °C until analysis. A chemiluminescent microparticle immunoassay on an Abbott ARCHITECT i2000SR Immunoassay Analyzer (Abbott Laboratories, Abbott Park, Illinois) was used to analyze the serum concentrations of free 3,5,3′-triiodothyronine (FT₃), free thyroxine (FT₄), and thyrotropin (TSH). Enzymatic methods using a LABOSPECT 008 automatic analyzer (Hitachi, Tokyo, Japan) were carried out to measure serum total cholesterol (TC), HDL cholesterol (HDL-C), and TG concentrations. The Friedewald formula was used to determine LDL cholesterol (LDL-C) concentrations: LDL-C = TC − HDL-C − TG/5 [40 (link)].
LPL latex agglutination turbidimetry (Immuno-Biological Laboratories [IBL], Gunma, Japan) was used to measure serum LPL concentrations [35 (link)]. A Human GPIHBP1 Assay Kit (IBL) based on sandwich ELISA was used to determine serum GPIHBP1 concentrations [36 (link)]. Finally, the Human Serum HTGL ELISA Kit (IBL) was used to measure serum HTGL concentrations [34 (link)].