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Coolsnap pro

Manufactured by Media Cybernetics
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The CoolSNAP-Pro is a high-performance digital camera designed for scientific and industrial applications. It features a sensitive, high-resolution image sensor capable of capturing detailed images and video. The camera is equipped with advanced cooling technology to reduce thermal noise, allowing for long exposure times and improved image quality. The CoolSNAP-Pro is a versatile and reliable imaging solution for a variety of research and industrial applications.

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Lab products found in correlation

Hoechst 33342, by Merck Group (5 mentions) Ds qi2, by Nikon (4 mentions) Eclipse te2000 s microscope, by Nikon (4 mentions) Image pro plus software, by Media Cybernetics (3 mentions) Dmlb microscope, by Leica (3 mentions) Acetone, by Merck Group (3 mentions) Hoechst 33342, by Media Cybernetics (2 mentions) Eclipse 400, by Nikon (2 mentions) Image pro plus, by Media Cybernetics (2 mentions) Axioskop 2 mot microscope, by Media Cybernetics (1 mentions) Hoechst 33342 dye, by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) Alexa fluor 488 anti mouse igg, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Dapi solution, by Vector Laboratories (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Image pro plus software version 4, by Media Cybernetics (1 mentions) Image pro plus version 4, by Media Cybernetics (1 mentions) Te2000s inverted microscope, by Nikon (1 mentions)

Close spelling variants of this product

CoolSNAP-Pro CF (4 citations) CoolSnap-Pro cf camera (4 citations) CoolSNAP-Pro digital camera (4 citations) CoolSNAP-Pro camera (4 citations)

29 protocols using coolsnap pro

1

Pulsed Laser Photothermolysis of Tumor Cells

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The experimental
setup is shown in Figure S3. A wavelength-tunable
pulsed Ti:sapphire laser pumped by a Q-switched Nd:YAG laser at a
repetition rate of 10 Hz (Lotis TII; Symphotic) was used for the photothermolysis
experiment. The laser system provides laser pulses with a 15 ns pulsewidth
and wavelength options of 1064 and 532 nm and is continuously tunable
from 700 to 960 nm. A laser wavelength was selected to match the absorption
peak of the nanoparticle applied in each experiment.
The laser
beam was adjusted by a beam expander and then directed onto live tumor
cells seeded in microplates, which were placed under a Leica microscope
(Lecia Microsystems, Wetzlar, Germany). The images were acquired using
a cooled CCD camera (CoolSNAPPro, Media Cybernetics, Rockville, MD).
The incident laser energy density of the sample was regulated by controlling
either the laser pulse energy or the beam size. The laser pulse energy
was measured by a pyroelectric energy meter (PE25-C, Ophir, North
Logan, UT). The laser energy fluence on cells was calculated according
to the laser beam incident angle and verified by measurement. The
laser light from a continuous-wave diode laser (15 Plus, Doimed, U.K.)
at a wavelength of 808 nm was also directed onto tumor cells for a
side-by-side comparison with the pulsed laser light at the same wavelength.
Ku G., Huang Q., Wen X., Ye J., Piwnica-Worms D, & Li C. (2018). Spatial and Temporal Confined Photothermolysis of Cancer Cells Mediated by Hollow Gold Nanospheres Targeted to Epidermal Growth Factor Receptors. ACS Omega, 3(5), 5888-5895.
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2

Hoechst 33342 Fluorescence Microscopy

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The apoptotic bodies were examined with Hoechst 33342 (Sigma-Aldrich), which is a nucleus-specific dye. All cells were stained with Hoechst 33342, and the images were captured under a Cool SNAP-Pro color digital camera (Media Cybernetics, Silver Spring, MD, USA) in a fluorescence microscope [44 (link)].
Zhen A.X., Hyun Y.J., Piao M.J., Fernando P.D., Kang K.A., Ahn M.J., Yi J.M., Kang H.K., Koh Y.S., Lee N.H, & Hyun J.W. (2019). Eckol Inhibits Particulate Matter 2.5-Induced Skin Keratinocyte Damage via MAPK Signaling Pathway. Marine Drugs, 17(8), 444.
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3

Detecting Apoptotic Bodies Using Hoechst 33342

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To detect the apoptotic body, the DNA-specific fluorescent dye Hoechst 33342 was used [40 (link)]. Cells were pre-treated with 20 µM DPHC, the inhibitors, or both for 1 h, followed by PM2.5 for another 24 h. The stimulated cells were stained with Hoechst 33342 (Sigma-Aldrich) and visualized using a fluorescence microscope. Then, the images of proportions of the apoptotic cells were acquired using a CoolSNAP-Pro color digital camera (Media Cybernetics, Rockville, MD, USA).
Zhen A.X., Piao M.J., Hyun Y.J., Kang K.A., Fernando P.D., Cho S.J., Ahn M.J, & Hyun J.W. (2019). Diphlorethohydroxycarmalol Attenuates Fine Particulate Matter-Induced Subcellular Skin Dysfunction. Marine Drugs, 17(2), 95.
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4

Measuring Cell Viability with JC3 and Hoechst Staining

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Cells were seeded in a 24 well plate at a density of 2×105 cells/mL. After 24 h of plating, the cells were treated with 6 μM JC3 and incubated for an additional 48 h at 37°C. After 48 h, cells were incubated with the DNA-specific fluorescent dye Hoechst 33342 (1.5 μL, 10 mg/mL) for 10 min at 37°C and visualized using a fluorescence microscope equipped with a Cool SNAP-Pro color digital camera (Media Cybernetics, Silver Spring, MD, USA).
Park J.E., Piao M.J., Kang K.A., Shilnikova K., Hyun Y.J., Oh S.K., Jeong Y.J., Chae S, & Hyun J.W. (2017). A Benzylideneacetophenone Derivative Induces Apoptosis of Radiation-Resistant Human Breast Cancer Cells via Oxidative Stress. Biomolecules & Therapeutics, 25(4), 404-410.
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5

Quantifying Apoptosis by Hoechst 33342 Staining

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The DNA-specific fluorescent dye Hoechst 33342 (1.5 µL, 10 mg/mL stock) was added to each well, and cells were incubated at 37 °C for 10 min. Stained cells were observed under a fluorescence microscope equipped with a CoolSNAP-Pro color digital camera (Media Cybernetics, Rockville, MD, USA). The extents of nuclear condensation and apoptotic body formation were evaluated. The apoptotic index was calculated as follows: (number of apoptotic cells in treated group/total number of cells in treated group)/(number of apoptotic cells in control group/total number of cells in control group).
Piao M.J., Kang K.A., Zhen A.X., Kang H.K., Koh Y.S., Kim B.S, & Hyun J.W. (2019). Horse Oil Mitigates Oxidative Damage to Human HaCaT Keratinocytes Caused by Ultraviolet B Irradiation. International Journal of Molecular Sciences, 20(6), 1490.
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6

Intracellular Calcium Responses in Airway Smooth Muscle Cells

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Human airway smooth muscle cells were seeded onto sterilized glass cover slips at a density of 25 000 cells per well in 6 well tissue culture plates in DMEM 10% FBS with PSA (Invitrogen). After one day, cells were serum deprived in DMEM 0.5% FBS 1%PSA medium for 3 days before analysis of intracellular calcium responses to 1 µM S1P using 10 µM Fura2-AM. To mediate loading of the Fura-2 AM, pluronic F127 (0.02%), along with the 10 µM Fura-2 AM was dissolved in HBSS for 30 minutes at 37°C. Any unloaded Fura-2 AM was washed out with HBSS. Cover slips were loaded into a Leiden chamber (Medical Systems, Greenville, NY) and an inverted fluorescent microscope with a 40X oil-immersion objective (Olympus, Tokyo, Japan) was used to measure signals emitted at 510 nm using a CCD camera (CoolSnapPro; Media Cybernetics, Bethesda, MD) controlled by Image Master software (Photon Technology International, Birmingham, NJ). Data was acquired as previously described [15] (link).
O’Sullivan M.J., Hirota N, & Martin J.G. (2014). Sphingosine 1-Phosphate (S1P) Induced Interleukin-8 (IL-8) Release Is Mediated by S1P Receptor 2 and Nuclear Factor κB in BEAS-2B Cells. PLoS ONE, 9(4), e95566.
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7

Apoptotic Index Determination by Fluorescence Microscopy

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Cells were seeded at 1.0×105 cells/well in a 24-well culture plate and incubated for 48 h. Cells were treated with Hoechst 33342 cell-permeable nuclear counterstain dye for 10 min, and images were acquired using a fluorescence microscope equipped with a CoolSnap-Pro color digital camera (Media Cybernetics, Rockville, MD, USA). The apoptotic index was calculated using a formula as described previously (Piao et al., 2019 (link)).
Boo S.J., Piao M.J., Kang K.A., Zhen A.X., Fernando P.D., Herath H.M., Lee S.J., Song S.E, & Hyun J.W. (2022). Comparative Study of Autophagy in Oxaliplatin-Sensitive and Resistant SNU-C5 Colon Cancer Cells. Biomolecules & Therapeutics, 30(5), 447-454.
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8

Hoechst 33342 Apoptosis Detection Assay

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The DNA-specific fluorescent dye Hoechst 33342 (Sigma-Aldrich) was used to detect apoptosis. The cells were seeded in a 24-well plate at a density of 1 × 105 cells/mL and incubated for 16 h. The cells were then treated with hesperidin (50 µM) and/or PM2.5 (50 µg/mL), BAF (10 nM), U0126 (50 nM), SB203580 (10 µM), and SP600125 (5 µM). After each treatment, the cells were stained with Hoechst 33342 (20 µM) and visualized using a fluorescence microscope equipped with a Cool SNAP-Pro color digital camera (Media Cybernetics, Silver Spring, MD, USA) [21 (link)].
Fernando P.D., Piao M.J., Kang K.A., Zhen A.X., Herath H.M., Kang H.K., Choi Y.H, & Hyun J.W. (2022). Hesperidin Protects Human HaCaT Keratinocytes from Particulate Matter 2.5-Induced Apoptosis via the Inhibition of Oxidative Stress and Autophagy. Antioxidants, 11(7), 1363.
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9

Comet Assay for DNA Damage Analysis

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U87 MG cells were treated with TMZ, CINN, or combinations of both for 48 and 72 h. After drug treatment, cells were harvested to examine DNA damage using the comet assay (single-cell gel electroporesis), as previously described (26 (link),27 (link)). Cells were washed with PBS and mixed with low-melting agarose (1:10) before being loaded onto microscope slides. Cell lysis was performed at 4 °C (alkaline comet assay) and 37 °C (neutral comet assay). After electrophoresis for 25 min at 25 °C, DNA was stained with DAPI and imaged using a Zeiss Axioskop 2 Mot microscope equipped with a digital camera (CoolSnap-Pro, Media Cybernetics, Carlsbad, CA, USA). DNA damage was determined by the cell head length or cell tail length (µm) and the percentage of DAPI-stained comet cells using the Image J and Open Comet 1.3 software. These experiments were repeated at least three times, with an average of 200 cells each time.
Tai S.H., Lin Y.W., Huang T.Y., Chang C.C., Chao L.C., Wu T.S, & Lee E.J. (2021). Cinnamophilin enhances temozolomide-induced cytotoxicity against malignant glioma: the roles of ROS and cell cycle arrest. Translational Cancer Research, 10(9), 3906-3920.
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10

Detecting Neuronal ROS Production

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DCFH-DA, a cell membrane-permeable fluorescein analog, was used to detect ROS production34 (link) in differentiated NSC-34 motor neurons. At the end of the experiments, cells were viewed with a Nikon Eclipse 400 upright microscope (Nikon Instruments) equipped with a CCD digital camera (Coolsnap-Pro, Media Cybernetics).
Petrozziello T., Secondo A., Tedeschi V., Esposito A., Sisalli M., Scorziello A., Di Renzo G, & Annunziato L. (2017). ApoSOD1 lacking dismutase activity neuroprotects motor neurons exposed to beta-methylamino-L-alanine through the Ca2+/Akt/ERK1/2 prosurvival pathway. Cell Death and Differentiation, 24(3), 511-522.
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