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Depo provera

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Depo-provera is a contraceptive product manufactured by Merck Group. It is a progestin-only injectable contraceptive that prevents pregnancy by inhibiting ovulation and thickening cervical mucus.

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Lab products found in correlation

Bromodeoxyuridine (brdu), by Merck Group (2 mentions) Thedotap, by Merck Group (2 mentions) Fty720, by Cayman Chemical (2 mentions) Acitretin, by Merck Group (1 mentions) Tamoxifen, by Merck Group (1 mentions) Tie2 gfp, by Jackson ImmunoResearch (1 mentions)

4 protocols using depo provera

1

Acitretin Administration for ZIKV Treatment

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After 6 d of treatment with 1 mg/mouse Depo-provera, 20 µl of 2 mM acitretin (Sigma-Aldrich) in DMSO (Hsia et al., 2008 (link)) was administered into the vaginal cavity at the indicated times either before or after i.vag. ZIKV inoculation. All mice were anesthetized in an isoflurane chamber before administering the drug.
Khan S., Woodruff E.M., Trapecar M., Fontaine K.A., Ezaki A., Borbet T.C., Ott M, & Sanjabi S. (2016). Dampened antiviral immunity to intravaginal exposure to RNA viral pathogens allows enhanced viral replication. The Journal of Experimental Medicine, 213(13), 2913-2929.
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2

Delayed Blastocyst Implantation in Mice

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This protocol was approved by the Institutional Animal Care and Use Committee (IACUC) of the University of California, Merced (Animal Welfare Assurance #A4561-01). A female mouse expressing GFP under Tie-2, (Tie-2 GFP; Jackson Laboratories) was mated with a male mouse expressing the Discomsoma sp. RFP under α-SMA RFP (from Dr Brenner, University of California, San Diego) and observed for a postcoital (pc) plug the following morning (Figure 1). The presence of a plug was considered day 0 of embryo development. We then treated the pregnant mouse in order to delay the implantation (up to 8 days pc) of the developing blastocysts using modified methods from previously published protocols.21 (link)–23 On day 2 pc, the pregnant female mouse was given an intraperitoneal injection of 10 μg of Tamoxifen (Sigma) dissolved in propylene glycol and a subcutaneous injection of 3 mg of Depo-Provera (Sigma) dissolved in phosphate buffered solution (PBS).
Glaser D.E., Burns A.B., Hatano R., Medrzycki M., Fan Y, & McCloskey K.E. (2014). Specialized mouse embryonic stem cells for studying vascular development. Stem Cells and Cloning : Advances and Applications, 7, 79-88.
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3

Immunogenicity of rAd5-gp140 and rAd5-SIINFEKL

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Replication deficient adenovirus 5 expressing HIVgp140 (rAd5-gp140) was
developed and kindly provided by Dr. Gary Nabel (Vaccine Research Center, NIAID,
Bethesda MD). Replication deficient adenovirus 5 expressing SIINFEKL
(rAd5-SIINFEKL) was first developed by Dr. S. E. Hensley in Jon
Yewdell’s and Jack Bennink’s laboratory (NIAID) and kindly
provided by Dr. Robert Seder in (NIAID). The FTY720 was purchased from Cayman
Chemical (Ann Arbor, MI). The SIINFEKL peptide was purchased from Polypeptide
Laboratory (San Diego, CA). The mutant E. coli labile toxin
(mLT) was a kind gift of Dr. John Clements (Tulane University, New Orleans). The
DOTAP and bromodeoxyuridine (BrdU) were purchased from SIGMA (St. Louis, MO).
The Depo-provera was purchased from the Department of Veterinary Medicine,
NIH.
Wang Y., Sui Y., Kato S., Hogg A.E., Steel J.C., Morris J.C, & Berzofsky J.A. (2015). Vaginal type-II mucosa is an inductive site for primary CD8+ T-cell mucosal immunity. Nature communications, 6, 6100.
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4

Immunogenicity of rAd5-gp140 and rAd5-SIINFEKL

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Replication deficient adenovirus 5 expressing HIVgp140 (rAd5-gp140) was
developed and kindly provided by Dr. Gary Nabel (Vaccine Research Center, NIAID,
Bethesda MD). Replication deficient adenovirus 5 expressing SIINFEKL
(rAd5-SIINFEKL) was first developed by Dr. S. E. Hensley in Jon
Yewdell’s and Jack Bennink’s laboratory (NIAID) and kindly
provided by Dr. Robert Seder in (NIAID). The FTY720 was purchased from Cayman
Chemical (Ann Arbor, MI). The SIINFEKL peptide was purchased from Polypeptide
Laboratory (San Diego, CA). The mutant E. coli labile toxin
(mLT) was a kind gift of Dr. John Clements (Tulane University, New Orleans). The
DOTAP and bromodeoxyuridine (BrdU) were purchased from SIGMA (St. Louis, MO).
The Depo-provera was purchased from the Department of Veterinary Medicine,
NIH.
Wang Y., Sui Y., Kato S., Hogg A.E., Steel J.C., Morris J.C, & Berzofsky J.A. (2015). Vaginal type-II mucosa is an inductive site for primary CD8+ T-cell mucosal immunity. Nature communications, 6, 6100.
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