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Alexa fluor 594 goat anti rat igg h l secondary antibody

Manufactured by Thermo Fisher Scientific
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Alexa Fluor 594 goat anti-rat IgG (H+L) secondary antibody is a fluorescently labeled secondary antibody that specifically binds to the heavy and light chains of rat immunoglobulin G (IgG). It is designed for use in various immunodetection techniques, such as immunofluorescence microscopy and flow cytometry.

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Lab products found in correlation

Rat monoclonal anti mouse cd8a primary antibody, by BD (2 mentions) Tcs sp5 confocal microscope, by Leica (2 mentions)

Close spelling variants of this product

Alexa Fluor 594 goat anti-rat IgG secondary antibody Alexa Fluor® 594 secondary antibody (H + L; Alexa Fluor 594 goat anti-rat IgG Alexa Fluor 594 goat anti-rat Anti-rat IgG Alexa Fluor 594 Alexa Fluor 594 anti-goat IgG Goat anti-rat IgG (H + L; Alexa Fluor 594 secondary antibody Alexa Fluor IgG H + L Alexa Fluor 594 anti-Rat

2 protocols using alexa fluor 594 goat anti rat igg h l secondary antibody

1

CD8+ T Cell Quantification in Tumor Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence staining of CD8 was performed on frozen mouse tumor tissue sections. Rat monoclonal anti-mouse CD8a primary antibody (BD Pharmingen™) and Alexa Fluor 594 goat anti-rat IgG (H+L) secondary antibody (Invitrogen) were used for the staining. Cell nuclei were stained with DAPI. Imaging was performed using a Leica TCS SP5 confocal microscope. Sixteen frames acquired with a 63X objective lens were used to calculate the cell number per mm2.
Veglia F., Tyurin V.A., Blasi M., De Leo A., Kossenkov A., Donthireddy L., Jerrick T.T., Schug Z., Basu S., Wang F., Ricciotti E., DiRusso C., Murphy M.E., Vonderheide R.H., Lieberman P.M., Mulligan C., Nam B., Hockstein N., Masters G., Guarino M., Lin C., Nefedova Y., Black P., Kagan V.E, & Gabrilovich D. (2019). Fatty acid transporter 2 reprograms neutrophils in cancer. Nature, 569(7754), 73-78.
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2

CD8+ T Cell Quantification in Tumor Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence staining of CD8 was performed on frozen mouse tumor tissue sections. Rat monoclonal anti-mouse CD8a primary antibody (BD Pharmingen™) and Alexa Fluor 594 goat anti-rat IgG (H+L) secondary antibody (Invitrogen) were used for the staining. Cell nuclei were stained with DAPI. Imaging was performed using a Leica TCS SP5 confocal microscope. Sixteen frames acquired with a 63X objective lens were used to calculate the cell number per mm2.
Veglia F., Tyurin V.A., Blasi M., De Leo A., Kossenkov A., Donthireddy L., Jerrick T.T., Schug Z., Basu S., Wang F., Ricciotti E., DiRusso C., Murphy M.E., Vonderheide R.H., Lieberman P.M., Mulligan C., Nam B., Hockstein N., Masters G., Guarino M., Lin C., Nefedova Y., Black P., Kagan V.E, & Gabrilovich D. (2019). Fatty acid transporter 2 reprograms neutrophils in cancer. Nature, 569(7754), 73-78.
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