Tetrandrine was purchased from Shanghai Ronghe Medical, Inc. (Shanghai, China) and dissolved in DMSO for use. DCFH-DA was obtained from Invitrogen (Carlsbad, CA). 3-Methyladenine (3-MA) and N-acetyl-L-cysteine were purchased from Sigma (St. Louis, MO). Acridine orange (AO), GAPDH antibody and HRP-conjugated secondary antibodies (goat anti-rabbit and goat anti-mouse) were purchased from Beyotime (Nantong, China). The antibody against microtubule-associated protein 1 light chain 3 (LC3) was purchased from Sigma (St. Louis, MO). The p62 antibody was obtained from Cell Signaling Technologies (Beverly, MA), and cathepsin D (CTSD) was acquired from Proteintech Group, Inc. (Chicago, IL).
Cathepsin d
Manufactured by Proteintech
Sourced in United States, China
Cathepsin D is a lysosomal aspartic protease that plays a role in protein degradation. It is involved in the breakdown of proteins and peptides within the lysosome.
Automatically generated - may contain errors
Lab products found in correlation
Dcfh da, by Thermo Fisher Scientific (2 mentions)
Hrp conjugated secondary antibodies goat anti rabbit and goat anti mouse, by Beyotime (2 mentions)
Antibody against microtubule associated protein 1 light chain 3, by Merck Group (2 mentions)
N acetyl l cysteine, by Merck Group (2 mentions)
P62 antibody, by Cell Signaling Technology (1 mentions)
3 methyladenine 3 ma, by Merck Group (1 mentions)
Sqstm1 p62, by Abcam (1 mentions)
Parkin, by Abcam (1 mentions)
β actin, by Proteintech (1 mentions)
Cytochrome c, by Proteintech (1 mentions)
Pink1, by Abcam (1 mentions)
Chloroquine diphosphate, by MedChemExpress (1 mentions)
Pki 402, by MedChemExpress (1 mentions)
Primary antibodies against lc3b, by Abcam (1 mentions)
Lamp1, by Proteintech (1 mentions)
Cathepsin b, by Proteintech (1 mentions)
Alpha tubulin, by Proteintech (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Rapamycin, by MedChemExpress (1 mentions)
P27kip1, by Cell Signaling Technology (1 mentions)
4 protocols using cathepsin d
1
Studying Autophagy Pathway Modulators
2
Autophagy and Mitochondrial Dynamics Assay
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MTT was purchased from Sigma‐Aldrich (USA). Chloroquine diphosphate, E‐64, PKI‐402 and rapamycin were purchased from MedChemExpress (USA). The primary antibodies against LC3B, SQSTM1/p62, TFEB, PINK1, parkin, Fis1, Drp1, Mfn1 and Mfn2 were purchased from Abcam (USA), and LAMP1, beta‐actin, alpha‐tubulin, cathepsin B, cathepsin D and cytochrome c were purchased from Proteintech (USA).
3
Tetrandrine-Induced Autophagy and Apoptosis
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Tetrandrine was purchased from Shanghai Ronghe Medical, Inc. (Shanghai, China) and was stored at −80°C as a powder and dissolved in dimethyl sulfoxide before use. 3-Methyladenine and N-acetyl-L-cysteine were purchased from Sigma (St. Louis, MO). DCFH-DA was obtained from Invitrogen (Carlsbad, CA). DAPT was obtained from Selleck (Shanghai, China). Acridine orange, NBT, the GAPDH antibody and HRP-conjugated secondary antibodies (goat anti-rabbit and goat anti-mouse) were purchased from Beyotime (Nantong, China). The antibody against microtubule-associated protein 1 light chain 3 (LC3) was purchased from Sigma (St. Louis, MO). CD14-FITC and CD11b-PE were obtained from BD Biosciences. Other antibodies were obtained from the following sources: p27kip1, PARP, p62, HES1, Notch1 were from Cell Signaling Technologies (Beverly, MA); CD14, cathepsin D (CTSD), and p21 were acquired from Proteintech Group Inc. (Chicago, IL).
4
Optimizing Autophagy Modulation for Cancer Therapy
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The reagents used in this study were purchased from Chinese suppliers: Z-VAD-fmk (CSNpharm, CSN15936, Chicago, USA), CQ (Sigma-Aldrich, C6628, St. Louis, MO, USA), NAC N-acetyl-L-cysteine (Beyotime, S0077, Shanghai, China), AO (Sigma-Aldrich, A6014), 3-MA (Selleck, S2767, Houston, Texas, USA), rapamycin (Selleck, S1039), Cisplatin (Selleck, S1166), and Dorsomorphin (Selleck, S7846). The following antibodies were also purchased from Chinese suppliers: GAPDH (Proteintech, 10,494–1-AP, Wuhan, China), cathepsin D (Proteintech, 21,327–1-AP), PCNA (Proteintech, 10,205–2-AP), Ki-67 (Proteintech, 27,309–1-AP), LAMP2 (Proteintech, 66,301–1-lg), p70s6K (Proteintech, 14,485–1-AP), tubulin (Proteintech,1124–1-AP), P21(Proteintech, 10,355–1- AP), cleaved-caspase9 (Asp175) (CST, 52873, Beverly, MA, USA), cleaved caspase 8 (Asp391) (CST,9496), Bax (CST 2774), Bid (CST,2003), Raptor (CST,2282), p-raptor(Ser792) (CST,2083), Rab7 (CST,9367), AMPK (CST, 5832), p-AMPK (CST, 2535), mTOR (CST, 2983), p-mTOR (CST,5536), p-p70s6K (CST,9234), LC3B (CST, 3868), LAMP1 (CST 15665), Atg7 (CST,8558), p62 (CST, 88588).
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