Pelco cu grids

Manufactured by Ted Pella

Sourced in United States

PELCO® Cu grids are copper transmission electron microscopy (TEM) sample support grids. They are designed to hold thin samples for examination under a TEM. The grids are made of copper and are available in various mesh sizes and styles to accommodate different sample preparation requirements.

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Lab products found in correlation

Orius sc1000 w camera, by Ametek (1 mentions) Jem 1011 transmission electron microscope, by JEOL (1 mentions) Item software, by Olympus (1 mentions) Quemesa digital camera, by Olympus (1 mentions) Jem 1400 tem, by JEOL (1 mentions)

Close spelling variants of this product

PELCO® Grids Cu grid

2 protocols using pelco cu grids

Transmission Electron Microscopy Analysis of Extracellular Vesicles

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A 10 μL drop of EVs in PBS was applied to nitrocellulose carbon-coated PELCO^® Cu grids (Ted Pella Inc., Redding, CA, USA) and incubated for 1 min. Liquid was removed by touching the paper edge. A 10 μL drop of 2% uranyl acetate was immediately applied to the grid, followed by 15 s incubation and drop removal by touching the paper edge. Samples were examined at 80 kV with a JEM-1011 transmission electron microscope (JEOL, Akishima, Japan) equipped with an Orius™ SC1000 W camera (Gatan Inc., Pleasanton, CA, USA).

Silachev D.N., Goryunov K.V., Shpilyuk M.A., Beznoschenko O.S., Morozova N.Y., Kraevaya E.E., Popkov V.A., Pevzner I.B., Zorova L.D., Evtushenko E.A., Starodubtseva N.L., Kononikhin A.S., Bugrova A.E., Evtushenko E.G., Plotnikov E.Y., Zorov D.B, & Sukhikh G.T. (2019). Effect of MSCs and MSC-Derived Extracellular Vesicles on Human Blood Coagulation. Cells, 8(3), 258.

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Visualizing Extracellular Vesicles by TEM

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A 10 μL drop of EV in PBS was applied to nitrocellulose carbon-coated PELCO® Cu grids (Ted Pella Inc., Redding, CA, USA) and incubated for 1 min. Liquid was removed by touching the top of the grid with filter paper. This grid was exposed to a 10 μL drop of 2% uranyl acetate, followed by 10 s incubation with further removal of the moisture by touching the grid with the filter paper. Samples were examined in a transmission electron microscope, JEOL JEM-1400 TEM (JEOL, Tokyo, Japan) attached to an Olympus Quemesa digital camera and analyzed by iTEM software (Olympus Soft Imaging Solutions GmbH, Munster, Germany).

Turovsky E.A., Golovicheva V.V., Varlamova E.G., Danilina T.I., Goryunov K.V., Shevtsova Y.A., Pevzner I.B., Zorova L.D., Babenko V.A., Evtushenko E.A., Zharikova A.A., Khutornenko A.A., Kovalchuk S.I., Plotnikov E.Y., Zorov D.B., Sukhikh G.T, & Silachev D.N. (2022). Mesenchymal stromal cell-derived extracellular vesicles afford neuroprotection by modulating PI3K/AKT pathway and calcium oscillations. International Journal of Biological Sciences, 18(14), 5345-5368.

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