Cytospin preparations (20,000 cells per slide) were prepared as previously described [14 (link), 20 (link)], fixed with 10% neutral-buffered formalin (NBF) for 10 min at room temperature, and stained for Keratin 5 (Covance Innovative Antibodies, Rabbit, 1:1000) and Keratin 14 (Thermo Scientific, Mouse, 1:500). Slides were counterstained with 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI). Images of at least 200 nucleated cells were acquired using Zeiss Imager.Z1 fluorescent microscope (Zeiss) and quantified using ImageJ software.
Imager z1 fluorescent microscope
Manufactured by Zeiss
Sourced in Germany
The Zeiss Imager.Z1 is a fluorescent microscope designed for high-resolution imaging. It features advanced optics and illumination systems to enable detailed visualization of fluorescently-labeled samples. The core function of the Imager.Z1 is to provide a platform for researchers to conduct fluorescence microscopy experiments.
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Lab products found in correlation
4 protocols using imager z1 fluorescent microscope
1
Keratin 5 and 14 Cytospin Assay
2
Tracheal Tissue Sectioning and Analysis
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Tracheal tissue sections (5 μm) were generated from paraffin‐embedded tissues and processed as described previously.22 All antibodies (K5, CCSP, BrdU, and ACT), and staining methods were previously described.19 EdU was detected using the ClickIt method and followed the manufacturer's instructions. Images were acquired using an upright Zeiss Imager Z1 fluorescent microscope and AxioVision software (Carl Zeiss) or an inverted Zeiss 200 M confocal microscope using Intelligent Imaging Innovations Inc. software. Cell type frequency was quantified as previously indicated22 and values were expressed as a percent of total epithelial cells. N = 6 tracheas/group.
3
Histological Evaluation of Respiratory Tissues
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Tissue sections (5 μm) from the trachea and the right lower lobe of lung were generated from paraffin embedded tissue. Sections were stained with hematoxylin and eosin. The study pathologist (DTM) graded the levels of dysplasia and performed all the histological analyses.
For immunofluorescence studies, antigens were detected with antibodies to K5, K14, CCSP, ACT, BrdU, p63, NGFR, and involucrin employing previously described staining methods [15 ]. Images were acquired using an upright Zeiss Imager Z1 fluorescent microscope and AxioVision software (Carl Zeiss).
For immunofluorescence studies, antigens were detected with antibodies to K5, K14, CCSP, ACT, BrdU, p63, NGFR, and involucrin employing previously described staining methods [15 ]. Images were acquired using an upright Zeiss Imager Z1 fluorescent microscope and AxioVision software (Carl Zeiss).
4
Comet Assay Protocol for DNA Damage
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