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Dcfh da ma0219

Manufactured by Meilun
Sourced in China

DCFH-DA (MA0219) is a fluorogenic probe that measures intracellular oxidative stress. It is a cell-permeable, non-fluorescent compound that is deacetylated by cellular esterases and then oxidized by reactive oxygen species (ROS) to produce the highly fluorescent compound 2',7'-dichlorofluorescein (DCF).

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2 protocols using dcfh da ma0219

1

Detailed Compound Acquisition for Bioassays

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PHZ (G1324044) was purchased from Aladdin company of Shanghai, China. 1-phenyl 2-thiourea (PTU, P7629) and Ethyl 3-aminobenzoate methanesulfonate (Tricaine, E10521) were purchased from Sigma-Aldrich company of United States. DCFH-DA (MA0219) and Aspirin (MB1790) were purchased from Dalian Meilun Biotechnology Company, China. MDA (S0131) was purchased from Beyotime Company of Shanghai, China. Cryptotanshinone was purchased from Winherb Medical Technology Company of Shanghai, China. Salvianolic acid B (B20261), Danshensu (B20254), Rosmarinic acid (B20862), Ferulic acid (B20007), Senkyunolide I (B21463) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). The purity of the six compounds are all greater than 98%.
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2

Analytical Protocols for Bioactive Compounds

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TNBS (MB5547), 5-ASA (MB7539), and DCFH-DA (MA0219) were purchased from Dalian Meilun Biotechnology Company, China. 1-phenyl 2-thiourea (PTU) (P7629) and Ethyl 3-aminobenzoate methanesulfonate (Tricaine, E10521) were purchased from Sigma-Aldrich company of USA. Puerarin, 3สน-methoxyPuerarin, daidzin, oroxindin, glycyrrhizic acid and esculin were purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China). Palmatine chloride, chlorogenic acid, oroxyloside, fraxin and phellodendrine were purchased from Shanghai Winherb Medical Technology Co., Ltd (Shanghai, China). Berberine chloride was obtained from Dalian Meilun BiotechnologyCo., Ltd (Dalian, China). Baicalin was obtained from Shanghai Aladdin Bio-Chem Technology Co., Ltd (Shanghai, China). The purities of all compounds were no less than 98%. HPLC-grade acetonitrile, methanol and formic acid were from Merk (Darmstadt, Germany). Deionized water was prepared with an Elga PURELAB flex system (ELGA LabWater, UK).
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