Cells were labeled with antibody or the corresponding isotype control in 1%BSA/PBS for 30 mins on ice. Cells were washed and analyzed using an Accuri C6 flow cytometer (BD Bioscience); histograms were generated using FlowJo software. Mean fluorescence values were normalized to the control cell line level. Antibodies used were CD82-Alexa-647 (Biolegend, ASL-24), CD53-PE (BioLegend, HI29), and Integrin β1-Alexa-647 (BioLegend, TS2/16). For active β1 integrin expression, cells were labeled with Ligand-induced binding site (LIBS) Anti-Integrin β1 Antibody (Millipore, HUTS-4) for 30 mins. Cells were washed and labeled with goat anti-mouse Alexa-647 secondary antibody (Invitrogen).
Cd82 alexa647
Manufactured by BioLegend
CD82-Alexa647 is a fluorescently labeled antibody that binds to the CD82 protein. CD82 is a cell surface protein involved in cell-cell interactions and signal transduction. The Alexa Fluor 647 dye is conjugated to the antibody, allowing for detection and analysis of CD82-expressing cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Asl 24, by BioLegend (2 mentions)
Cd53 pe, by BioLegend (2 mentions)
Integrin β1 alexa 647, by BioLegend (2 mentions)
Anti integrin β1 antibody, by Merck Group (2 mentions)
Goat anti mouse alexa 647 secondary antibody, by Thermo Fisher Scientific (2 mentions)
Accuri c6, by BD (2 mentions)
Annexin 5 fitc, by BioLegend (2 mentions)
Lsm 800 airyscan, by Zeiss (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
5 protocols using cd82 alexa647
1
Flow Cytometric Analysis of Cell Surface Markers
2
Annexin V and CD82 Imaging
3
Annexin V and CD82 Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Control or CD82OE cells were treated as indicated for 48 hours. Cells were blocked in 3%BSA in PBS followed by incubation with primary antibodies (AnnexinV-FITC, 1:200, Biolegend; CD82-Alexa647, 1:200, Biolegend) and DAPI (Invitrogen). Cells were washed and imaged with a Zeiss airyscan (LSM 800) system (Carl Zeiss) using excitation wavelengths of 405, 488 or 633 nm and a 63X/1.2 numerical aperture oil immersion objective.
4
Flow Cytometric Analysis of Cell Surface Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were labeled with antibody or the corresponding isotype control in 1%BSA/PBS for 30 mins on ice. Cells were washed and analyzed using an Accuri C6 flow cytometer (BD Bioscience); histograms were generated using FlowJo software. Mean fluorescence values were normalized to the control cell line level. Antibodies used were CD82-Alexa-647 (Biolegend, ASL-24), CD53-PE (BioLegend, HI29), and Integrin β1-Alexa-647 (BioLegend, TS2/16). For active β1 integrin expression, cells were labeled with Ligand-induced binding site (LIBS) Anti-Integrin β1 Antibody (Millipore, HUTS-4) for 30 mins. Cells were washed and labeled with goat anti-mouse Alexa-647 secondary antibody (Invitrogen).
5
Multicolor Immunofluorescence Imaging of Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were fixed with 4% paraformaldehyde and then blocked/permeabilized with 1%BSA/PBS/0.2%Tween. Cells were then incubated with primary antibodies (CD82-Alexa647, 1:125, Biolegend ASL-24; PKCα, 1:200, abcam, Y124). Cells were then labeled with a rabbit-Alexa488 secondary antibody (1:200, Invitrogen). Cells were imaged by laser scanning confocal microscopy with a Zeiss Axiovert 100 M inverted microscope (LSM 510) system (Carl Zeiss, Jena, Germany) using an excitation wavelength of 488 or 633 nm and a 63X/1.2 numerical aperture oil immersion objective. Image analysis was performed using the Zeiss LSM 510 software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!