Egg yolk phosphatidylcholine (PC), phosphatidylglycerol (PG), cholesterol (Chol), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine and paclitaxel were purchased from Sigma (St-Quentin-Fallavier, France). 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (Mal-PEG) and DOTAP were purchased from COGER (Paris, France). Docetaxel was purchased from VWR (Fontenay-sous-Bois, France). DIR, a fluorophore tag, was purchased from Caliper (Villebon-sur-Yvette, France). 2-Iminothiolane (Traut’s reagent) and Draq5 were purchased from Fisher Scientific (Illkirch-Graffenstaden, France). QuantiBRITE phycoerythrin (PE) and PE Mouse Anti-Human HER-2/neu were purchased from BD Biosci-ences (San Jose, CA, USA). Trastuzumab (Herceptin) was kindly given by Genentech (South San Francisco, CA, USA). All other reagents were of analytical grade.
Docetaxel
Manufactured by Avantor
Sourced in France
Docetaxel is a chemical compound used as a laboratory reagent. It functions as a cytotoxic agent, inhibiting cell division and promoting cell death. Docetaxel is commonly utilized in research and development applications.
Automatically generated - may contain errors
Lab products found in correlation
1 2 distearoyl sn glycero 3 phosphoethanolamine, by Merck Group (3 mentions)
Paclitaxel, by Merck Group (3 mentions)
Phosphatidylglycerol, by Merck Group (2 mentions)
2 iminothiolane traut s reagent, by Thermo Fisher Scientific (2 mentions)
Egg yolk phosphatidylcholine, by Merck Group (2 mentions)
Trastuzumab, by Genentech (2 mentions)
Pe mouse anti human her 2 neu, by BD (1 mentions)
Quantibrite phycoerythrin pe, by BD (1 mentions)
Draq5, by Thermo Fisher Scientific (1 mentions)
Trastuzumab herceptin, by Genentech (1 mentions)
Etoposide, by Cell Signaling Technology (1 mentions)
4 protocols using docetaxel
1
Liposomal Formulation for Targeted Cancer Therapy
2
Isolating Chemoresistant Cells from PC3
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Prior to drug treatment, the lethal dose 50 (LD50) values of three chemotherapeutics (cisplatin, docetaxel, and etoposide) were evaluated from dose response curves obtained via an alarmarBlue assay (VWR, #76285-554). Sub-confluent cultures of PC3 cells were treated with [LD50] cisplatin (6 µM; Millipore Sigma, #232120-50MG), docetaxel (5 nM; Cell Signaling Technology, #9886), or etoposide (25 µM; Cell Signaling Technology, #2200S) and cultured for 72 h under standard conditions. Large cells were then sterilely isolated via size-restricted filtration with a pluriStrainerⓇ 15 µm cell strainer (pluriSelect, #45-50015-03) and replated for culturing.
3
Liposomal Paclitaxel and Docetaxel Formulations
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Egg yolk phosphatidylcholine (PC), phosphatidylglycerol (PG), cholesterol (Chol), 1,2-distearoyl-sn-glycero- 3-phosphoethanolamine, and paclitaxel were purchased from Sigma-Aldrich Co (St Louis, MO, USA). 1,2-Distearoyl- sn-glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)]-2000 (Mal-PEG) was purchased from Coger (Paris, France). Docetaxel was purchased from VWR International (Fontenay-sous-Bois, France). DiR and DiL fluorophore tags were purchased from PerkinElmer Inc and Thermo Fisher Scientific (Waltham, MA, USA). 2-Iminothiolane (Traut’s reagent) was also purchased from Thermo Fisher Scientific. Trastuzumab was kindly provided by Genentech (South San Francisco, CA, USA). All other reagents were of analytical grade.
4
Lipid-Based Nanocarrier Formulation and Characterization
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Egg yolk phosphatidylcholine (PC), phosphatidylglycerol (PG), cholesterol (Chol), 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine (PEG) and Paclitaxel were purchased from Sigma (St Quentin-Fallavier, France). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000 (Mal-PEG were purchased from Coger (Paris, France). Docetaxel was purchased from VWR (Fontenay sous bois, France) and DiD fluorescent tag from Thermo Fisher (Illkirch, France). Trastuzumab (Herceptin) was kindly given by Genentech (South San Francisco, CA, USA). Anti-human IgG PE (clone L1C1) and a prototyped human IgG kappa light chains quantification kit for submicron particles (e.g., immunoliposomes) were provided by Biocytex (Marseille, France) as described below. All other reagents were of analytical grade.
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