Cells were lysed in lysis buffer (50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 1% Triton X-100, 10% glycerol) supplemented with Complete protease inhibitor tablet (Roche) on ice. Lysates were boiled in 1x NuPAGE lithium dodecyl sulfate sample buffer and subjected to SDS-PAGE using the Novex system (Invitrogen) following the manufacturer’s instructions, transferred to nitrocellulose membranes using the iBlot transfer system (Invitrogen), blocked in 5% nonfat milk in Tris-buffered saline/0.1% Tween-20 (TBST) for 1 hour at room temperature, and incubated with primary antibodies in TBST overnight at 4°C. The antibody for UHRF1 (Purified Mouse Anti-ICBP90) was from BD Biosciences (Cat. 612264). The beta-actin antibody was from Thermo (MA1–140 Invitrogen beta-actin antibody, 15G5A11/E2).
Purified mouse anti icbp90
Manufactured by BD
Purified Mouse Anti-ICBP90 is a laboratory reagent used for the detection and analysis of ICBP90 (Inverted CCAAT Box Binding Protein of 90 kDa) in various biological samples. It is a purified mouse monoclonal antibody that specifically binds to ICBP90, a protein involved in cellular processes.
Automatically generated - may contain errors
2 protocols using purified mouse anti icbp90
1
Western Blot Analysis of UHRF1
2
Western Blot Analysis of UHRF1
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