Rabbit anti cd11c

Formalin-fixed, paraffin-embedded slides were deparaffinized and sequentially rehydrated using ethanol. The slides were immersed in antigen retrieval solution (Dako, Santa Clara, CA) and incubated for antigen retrieval at high pressure using a cooker. After cooking, the slides were incubated with 2.5% horse serum in phosphate-buffered saline (PBS) for blocking and then incubated overnight at 4°C with the primary antibodies. Mouse anti–SARS-CoV-2 nucleocapsid (Sino Biological, Beijing, China), rabbit anti-CD11c (Cell Signaling Technology), rabbit anti-CD19 (Cell Signaling Technology), rat anti-CD3 (Abcam), rat anti-MHCII (eBioscience), rabbit anti-podoplanin (PDPN; Santa Cruz Biotechnology, Dallas, TX), goat anti–surfactant protein C (SP-C; Santa Cruz Biotechnology), rabbit anti-CD31 (Abcam), and rabbit anti–transmembrane protein 173 (TMEM173; Proteintech, Rosemont, IL) were used as primary antibodies. Each primary antibody derived from diverse species was detected with the use of Alexa488-conjugated, Alexa568-conjugated, and Alexa647-conjugated secondary antibodies (secondary antibodies all from Invitrogen, Waltham, MA). Nuclear staining was performed with the use of DAPI (Abbkine, Wuhan, China). Confocal images were taken with a confocal microscope (Ts2; Nikon, Tokyo, Japan) of Kangwon Center for System Imaging.