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Rat anti cd34

Manufactured by Abcam
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Sourced in United Kingdom, United States

Rat anti-CD34 is an antibody that recognizes the CD34 antigen, a transmembrane phosphoglycoprotein expressed on the surface of hematopoietic stem and progenitor cells. This antibody can be used for the identification and isolation of CD34-positive cells.

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Lab products found in correlation

Rat anti cd31, by Abcam (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Rabbit anti mouse cd206, by Abcam (1 mentions) Chicken anti gfp, by Abcam (1 mentions) Goat anti ephb4, by R&D Systems (1 mentions) Axio imager a2 microscope, by Zeiss (1 mentions) Goat anti endomucin, by R&D Systems (1 mentions) Mouse anti alpha smooth muscle actin, by Merck Group (1 mentions) Rat anti pecam1, by BD (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Rat anti-mouse CD34 Rat monoclonal anti-CD34 antibody and monoclonal anti-CD68 antibody PE-conjugated rabbit anti-rat CD34 Anti-CD34

4 protocols using rat anti cd34

1

Immunohistochemical Analysis of PGIS and CD34

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Paraformaldehyde-fixed, paraffin-embedded, 2-μm-thick sections were deparaffinized in xylene and rehydrated in a graded alcohol series. To retrieve the antigen, sections were heated in citrate buffer (pH 6.0) for 15 min at 120 °C by means of an autoclave. Sections were then blocked with 5% BSA and incubated overnight at 4 °C with the following primary antibodies: mouse anti-PGIS (1:100; Cayman Chemical) and rat anti-CD34 (1:200; Abcam, Cambridge, UK). For fluorescent visualization of the bound primary antibodies, sections were further incubated with the appropriate Cy3-conjugated or FITC-conjugated secondary antibodies (1:200; Jackson ImmunoResearch Laboratories) for 1 h at room temperature.
Cao Y., Guan Y., Xu Y.Y, & Hao C.M. (2018). Endothelial prostacyclin protects the kidney from ischemia-reperfusion injury. Pflugers Archiv, 471(4), 543-555.
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2

Immunohistochemical Analysis of Tissue Samples

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Tissue samples were stained with rat anti‐mouse MAC2 (1:200; Cedarlane Corp., Burlington,), rabbit anti‐mouse CD206 (1:300; Abcam,), rat anti‐CD31 (1:200, Abcam), rat anti‐CD34 (1:200, Abcam), rabbit anti‐mouse perilipin (1:400; Progen, Heidelberg, Germany) and chicken anti‐GFP (1:200, Abcam) primary antibodies. After washing, the samples were incubated with donkey anti‐rat Alexa Fluor 555 IgG (Abcam) and goat anti‐rabbit Alexa Fluor 430 IgG (Invitrogen, ) antibodies. Nuclei were stained with DAPI (Sigma, ).
Li Y., Chen X., Liu L., Chen Y., Bi X., Chen Y., Zou J., Wang Z., Dong Z, & Lu F. (2022). Alternatively activated macrophages at the recipient site improve fat graft retention by promoting angiogenesis and adipogenesis. Journal of Cellular and Molecular Medicine, 26(11), 3235-3242.
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3

Tissue Fixation and Immunostaining Protocols

Cited 1 time
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Embryos harvested from timed matings were fixed by immersion and lungs from postnatal mice were inflation fixed using 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS). Following overnight immersion in 4% PFA/PBS, fixed tissue was processed according to standard protocols for paraffin or frozen embedding. Hematoxylin and eosin (H&E) staining, immunohistochemistry, and immunofluorescence were performed on tissue sections (5–10 μm) as previously described (Lange et al., 2009 (link)). Primary antibodies included guinea pig anti-Sox17 (Seven Hills Bioreagents), goat anti-endomucin (R&D Systems), rat anti-Pecam-1 (BD Pharmingen), GSL-IB4-biotin (Vector Labs), rat anti-CD34 (Abcam), goat anti-EphB4 (R&D Systems), and mouse anti-alpha smooth muscle actin (Sigma). Fluorophore-conjugated secondary antibodies included Alexa Fluor-488 and Alexa Fluor-594 (Jackson ImmunoResearch and Life Technologies). For fluorescent stains, sections were stained with DAPI and mounted with ProLong Gold anti-fade reagent following antibody labeling (Invitrogen). Bright-field images were obtained using a Zeiss Axio ImagerA2 microscope equipped with AxioVision Software. Fluorescent images were obtained using a Nikon A1Rsi inverted laser confocal microscope and analyzed using Imaris software (Bitplane Scientific Software).
Lange A.W., Haitchi H.M., LeCras T.D., Sridharan A., Xu Y., Wert S.E., James J., Udell N., Thurner P.J, & Whitsett J.A. (2014). Sox17 is required for normal pulmonary vascular morphogenesis. Developmental biology, 387(1), 109-120.
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4

Immunofluorescence Staining of Tissue Samples

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For immunofluorescence staining, tissue samples were incubated with rat anti-CD31 (1:200; Abcam, Cambridge, MA, USA), rat anti-CD34 (1:200, Abcam) and rabbit anti-mouse perilinpin (1:400; Progen, Heidelberg, Germany) antibodies. After washing, the samples were incubated with donkey anti-rat Alexa Fluor 555 IgG (Abcam) and goat anti-rabbit Alexa Fluor 430 IgG (Invitrogen, North Ryde, NSW, Australia) antibodies. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma, St. Louis, MO, USA) [18 (link),19 (link)].
Li Y., Bi X., Wu M., Chen X., Zhan W., Dong Z, & Lu F. (2023). Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration. Burns & Trauma, 11, tkad002.
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