Antibodies to hiv p24

All reagents were purchased from Sigma (St. Louis, MO) except in the places that are indicated otherwise. HIV_ADA, TAK779, T20, soluble CD4 (sCD4), were from the NIH AIDS Research and Reference Reagent Program (Germantown, MD). Primers and probes were obtained from Biosynthesis (Lewisville, TX) and PNAbio (Newbury Park, CA). Medium, penicillin/streptomycin (P/S), dyes and secondary antibodies were obtained from Thermo-Fisher (Waltham, MA). Human AB serum and FBS were from Lonza (Walkersville, MD). HEPES was from USB (Cleveland, OH). HIV-p24 ELISA was obtained from Perkin-Elmer (Waltham, MA). Antibodies to HIV-p24 were obtained from Genetex (Irvine, CA). All other antibodies were purchased from Sigma, Santa Cruz (Santa Cruz, CA) or Abcam (Cambridge, MA). Purified mouse IgG_2B and IgG₁ myeloma protein were from Cappel Pharmaceuticals, Inc. TUNEL was obtained from Roche Ltd (Germany). All experiments were performed under the regulations of Rutgers University and the NIH.

Unless otherwise indicated, all reagents were purchased from Sigma (St. Louis, MO). HIV_ADA, CEM cells, and antiretrovirals were from the NIH AIDS Research and Reference Reagent Program (Germantown, MD). Medium, penicillin/streptomycin (P/S), dyes and secondary antibodies were obtained from Thermo-Fisher (Waltham, MA). Human AB serum and FBS were from Lonza (Walkersville, MD). HEPES was from USB (Cleveland, OH). HIV-p24 ELISA was obtained from Perkin-Elmer (Waltham, MA). Antibodies to HIV-p24 were obtained from Genetex (Irvine, CA). All other antibodies were purchased from Sigma, Santa Cruz (Santa Cruz, CA) or Abcam (Cambridge, MA). Purified mouse IgG_2B and IgG₁ myeloma protein were from Cappel Pharmaceuticals, Inc. TUNEL was obtained from Roche Ltd (Germany). All experiments were performed under the regulations of Rutgers University and the NIH.