WT and Irf2−/− mice were treated i.p. with anti-IFNAR1 antibody (clone MAR1-5A3; Sheehan et al., 2006 (link)) before SVN infection on day −1 (500 µg or 1 mg) or on day 2 p.i. (500 µg) or with mouse IgG1 isotype control (clone MOPC21; Bio X Cell) on day −1 before virus infection. In some experiments, WT mice were depleted of NK cells with anti-NK1.1 antibody (clone PK136; Bio X Cell) or treated with mouse IgG2a isotype control (clone C1.18.4.; Bio X Cell) 1 d before infection (300 µg). For MNP depletion, WT and Irf2−/− mice were treated i.p. with 200 µl clodronate or PBS liposome on day −3 before SVN infection.
Mouse igg2a isotype control clone c1.18.4
Manufactured by BioXCell
Mouse IgG2a isotype control (clone C1.18.4) is a laboratory reagent used as a control in immunological experiments. It serves as a reference to distinguish specific antibody binding from non-specific background signals.
Automatically generated - may contain errors
Lab products found in correlation
Mouse igg1 isotype control clone mopc 21, by BioXCell (2 mentions)
Anti ifnar1 antibody clone mar1 5a3, by BioXCell (1 mentions)
Anti nk1.1 antibody clone pk136, by BioXCell (1 mentions)
Anti il 4 clone 11b11, by BioXCell (1 mentions)
Anti il 9 clone 9c1, by BioXCell (1 mentions)
Anti ifn γ clone xmg1, by BioXCell (1 mentions)
Dig nhs ester, by Thermo Fisher Scientific (1 mentions)
Anti dig ab clone 9h27l19, by Thermo Fisher Scientific (1 mentions)
Rat igg2a isotype control clone 2a3, by BioXCell (1 mentions)
4 protocols using mouse igg2a isotype control clone c1.18.4
1
Interferon Receptor Blockade and Cell Depletion in Viral Infection
2
Cytokine Neutralization in Tumor Models
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Cytokine neutralization was achieved by intraperitoneal injections (200 µg per mouse) of anti-IFN-γ (clone XMG1.2, BioXCell), anti-IL-9 (clone 9C1, BioXCell), anti-IL-4 (clone 11B11, BioXCell) or anti-IL-17 (clone 17F3, BioXCell) antibodies 1 day before tumor cell implantation and then three times a week. Alternatively, IFN-γ neutralization in MC38 tumor-bearing mice was achieved by intraperitoneal injections (200 µg per mouse) of anti-IFN-γ on days 4, 5, 7, 9, 10, 12, and 14 after tumor cell implantation. As control for anti-IL-17 or anti-IFN-γ, anti-IL-4, and anti-IL-9, rat IgG1 isotype control (clone HRPN, BioXCell), mouse IgG1 isotype control (clone MOPC-21, BioXCell), and mouse IgG2a isotype control (clone C1.18.4, BioXCell) were respectively used.
3
Visualizing ICAM-1 in Tumor Microenvironment
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Anti‐ICAM‐1 Ab (clone R6‐5‐D6; Bio X Cell) or its mouse IgG2a isotype control (clone C1.18.4; Bio X Cell) (1 mg) was labeled with DIG by incubating 1 mg Ab and 50 μg DIG‐NHS‐ester (Thermo Fisher Scientific) using a similar method to IR700 conjugation. The resulting DIG‐labeled Abs were abbreviated as ICAM‐1‐DIG and isotype‐DIG, respectively. Tumor‐bearing mice were injected with ICAM‐1‐DIG or isotype‐DIG (100 μg) into the lateral tail vein. Tumors were harvested 24 h after injecting DIG‐labeled Abs. The distribution of DIG‐labeled Abs was analyzed in FFPE sections by multiplex immunohistochemistry using anti‐DIG Ab (clone 9H27L19; Thermo Fisher Scientific).
4
Flt3L and CD40 Agonist Immunotherapy
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After randomization based on tumor volume, mice were injected intraperitoneally (ip) with 30 µg recombinant Flt3L (BioXCell - BE0098) or vehicle (HBSS) daily for 12, 9, or 6 days till sacrifice for E0771, 9 days till sacrifice for TS/A and 7 days till sacrificed for LLC. For CD40 agonist treatments, a single dose of 100 μg of CD40 (clone: FGK4.5; BioXCell) agonist antibody or rat IgG2a isotype control (clone 2A3; BioXCell) was administered ip. For Treg-depletions, a single dose of 20 μg of αCD25 (ONCC4, kindly provided by Oncurious) or mouse IgG2a isotype control (clone C1.18.4; BioXCell) was administered ip.
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