The assay was carried out using a Trace 1310 series GC with a TSQ8000 MS detector (Thermo Fisher Scientific Co. Ltd., Waltham, Massachusetts, MA, USA). A 1 μL portion of extract was injected in splitless mode onto the column. Chromatographic separation was performed on a TR-5ms capillary column (30 m × 0.25 mm ID; DF = 0.25 μm; Thermo Fisher Scientific Co. Ltd., Waltham, MA, USA). Helium was used as the carrier gas at a constant flow rate of 1 mL/min through the column. The injector temperature was set at 280 °C and the injector temperature was 280 °C. The oven program was as follows: 50 °C for 2 min, linear ramp at a rate of 20 °C·min−1 to 200 °C, followed with a linear ramp at a rate of 5 °C·min−1 to 300 °C, held at 300 °C for 10 min. The transfer line temperature was 280 °C. The data was acquired and processed using Thermo Scientific Xcalibur data handling software.
Tr 5ms capillary column
Manufactured by Thermo Fisher Scientific
Sourced in United States
The TR-5MS capillary column is a gas chromatography (GC) column designed for separation and analysis of a wide range of organic compounds. It features a 5% phenyl-95% dimethylpolysiloxane stationary phase, providing excellent inertness and thermal stability. The column is suitable for use in various GC applications.
Automatically generated - may contain errors
Lab products found in correlation
Trace 1310 series gc, by Thermo Fisher Scientific (3 mentions)
Tsq8000 ms detector, by Thermo Fisher Scientific (2 mentions)
Isq ms, by Thermo Fisher Scientific (2 mentions)
Trace 1310 gc, by Thermo Fisher Scientific (2 mentions)
Trace gc ultra system, by Thermo Fisher Scientific (1 mentions)
Dsq 2 gc, by Thermo Fisher Scientific (1 mentions)
Beh column, by Waters Corporation (1 mentions)
Uplc q tof ms, by Waters Corporation (1 mentions)
Tsq 8000 ms, by Thermo Fisher Scientific (1 mentions)
Trace ms quadrupole mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Focus gas chromatography, by Thermo Fisher Scientific (1 mentions)
Polaris q, by Thermo Fisher Scientific (1 mentions)
Trace gc 2000, by Thermo Fisher Scientific (1 mentions)
12 protocols using tr 5ms capillary column
1
GC-MS Analysis of Organic Compounds
2
Floral Volatile Organic Compound Analysis
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Floral VOC samples were analyzed with a Thermo Trace 1310 GC coupled to a Thermo ISQ MS with electron ionization (EI) at 70.0 eV at 250°C, using helium as the carrier gas at 1.0 ml/min with the injector temperature set at 250°C. Oven conditions included an initial temperature of 40°C followed by an immediate ramp of 3°C min−1 to 200°C. Available standards, samples, and a continuous series of n‐alkanes (C8–C20; Sigma‐Aldrich) were injected (1 μl) in the split mode onto a TR‐5MS capillary column (30 m × 0.25 mm I.D., film thickness 0.25 μm; Thermo Fisher Scientific). Compounds were identified with retention time matches to pure standards, mass spectra, and/or linear retention indexes calculated with the alkane series (Adams, 2001 ; El‐Sayed, 2021 ; NIST, 2008 ). Standard curves of available compounds were used to calculate final VOC results, which were expressed as ng compound g−1 DW hr−1.
3
GC-MS Analysis of Compound Mixtures
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The GC-MS analysis was conducted on a Trace GC Ultra system equipped with an AS 3000 auto-sampler, a split/splitless injector, and TSQ Quantum XLS MS detector with triple quadrupole (Thermo Fisher Scientific, Waltham, MA, USA). The TR-5 MS capillary column (30 m × 0.25 mm, 0.25 μm film thickness) was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
The injection temperature and ion source temperature were 250 °C. The oven temperature program was maintained at 50 °C for 1 min, increased to 200 °C (rate: 8 °C/min), maintained at 200 °C for 5 min, increased to 280 °C (rate: 10 °C/min), and maintained for 5 min. Helium was used as the carrier gas (flow rate: 1 mL/min). Sample injection volume and split ratio were 1 μL and 50:1, respectively. The ionizing energy was 70 eV. The chromatograms were obtained by collecting the total ion currents in the scan range of m/z 50–550. Data were acquired using the Xcalibur 2.2 software.
The injection temperature and ion source temperature were 250 °C. The oven temperature program was maintained at 50 °C for 1 min, increased to 200 °C (rate: 8 °C/min), maintained at 200 °C for 5 min, increased to 280 °C (rate: 10 °C/min), and maintained for 5 min. Helium was used as the carrier gas (flow rate: 1 mL/min). Sample injection volume and split ratio were 1 μL and 50:1, respectively. The ionizing energy was 70 eV. The chromatograms were obtained by collecting the total ion currents in the scan range of m/z 50–550. Data were acquired using the Xcalibur 2.2 software.
4
GC-MS Analysis of Camphor and Borneol in Cinnamomum camphora Leaves
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The assay was carried out using a Trace 1310 series GC with a TSQ8000 MS detector (Thermo Fisher Scientific, United States). A TR-5 ms capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness; Thermo Fisher Scientific, United States) was used. The carrier gas for GC was helium at a flow rate of 1.0 mL⋅min–1. The oven program was as follows: 50°C for 2 min, linear ramp up at a rate of 5°C⋅min–1 to 230°C, held at 230°C for 5 min, followed with a linear ramp up at a rate of 10°C⋅min–1 to 300°C, held at 300°C for 2 min. The injector temperature and transfer line temperature were 280°C.
A chiral column, Agilent CycloSil-B (30 m × 0.25 mm i.d., 0.25 μm film thickness), was used to identify the chirality of the assay product and the content of borneol and camphor in C. camphora leaves. The carrier gas for GC was helium at a flow rate of 1.0 mL⋅min–1. The oven program was as follows: 50°C for 2 min, followed by a gradient from 50°C to 180°C at 5°C⋅min–1, then 10°C⋅min–1 to 230°C, held at 230°C for 2 min. The injector temperature was 200°C, and the transfer line temperature was 230°C.
A chiral column, Agilent CycloSil-B (30 m × 0.25 mm i.d., 0.25 μm film thickness), was used to identify the chirality of the assay product and the content of borneol and camphor in C. camphora leaves. The carrier gas for GC was helium at a flow rate of 1.0 mL⋅min–1. The oven program was as follows: 50°C for 2 min, followed by a gradient from 50°C to 180°C at 5°C⋅min–1, then 10°C⋅min–1 to 230°C, held at 230°C for 2 min. The injector temperature was 200°C, and the transfer line temperature was 230°C.
5
Phytochemical Profiling of C. tinctoria
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To understand which phytochemicals are present in the methanolic extract (MEC) and n-butanol fraction of Chrozophora tinctoria (NBFC), both samples were subjected to gas chromatography–mass spectrometry [33 (link)]. The plant samples were checked using a Thermo Scientific (DSQ-II) GC, furnished with a 30 m long TR-5MS capillary column and a 0.25 µm thick film with 0.25 mm of internal diameter. Helium was used as a carrier gas with a flow rate of 1 mL/min. The injection device was run in a split mode at 250 °C. The sample was injected, 1 µL at a time, with an initial oven temperature of 50 °C that was maintained for 2 min followed by gradually elevating the temperature to 150 °C at a rate of 8 °C/min. Ultimately, the temperature was increased to 300 °C at a speed of 15 °C/min and sustained for 5 min [34 (link),35 ]. The mass spectrometry was performed in full scan mode to obtain information about mass fragments and mass/charge (m/z) ratio in the range of 50–600.
6
Hydrogenation and Dehydrogenation Analysis
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The products of hydrogenation and dehydrogenation reactions were analyzed using a CrystaLlux-4000M chromatograph with a flame-ionization detector using a ZB-5 (Phenomenex, Torrance, CA, USA) and TR-FFAP capillary columns (Thermo Scientific, Waltham, MA, USA). The analysis was performed in a programmable mode of 70–220 °C at a heating rate of 6 °C/min. For more detailed identification of semi-hydrogenated products and reaction by-products, separate liquid samples were analyzed using a FOCUS DSQ II chromate-mass-spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) with a TR-5MS capillary column (Thermo, Waltham, MA, USA). The conversion in hydrogenation and dehydrogenation (X) was calculated by the formula: X = (c0 − c)/c0 × 100%, where c0 and c are the initial and final concentrations of the substrate.
The selectivity (S) was calculated by the formula: S(i) = ∑ c(i)/∑ c(k) × 100%, where ∑ c(i) and ∑ c(k) are the sums of concentrations of a group of products and all the products, respectively.
The selectivity (S) was calculated by the formula: S(i) = ∑ c(i)/∑ c(k) × 100%, where ∑ c(i) and ∑ c(k) are the sums of concentrations of a group of products and all the products, respectively.
7
Analytical Profiling of Bioactive Compounds
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Trace 1310 series GC and TSQ8000 MS were used to detect substrates and products (Thermo Fisher Scientific Co., Ltd., Waltham, MA, USA). Chromatographic separation was performed on a TR-5ms capillary column (30 m * 0.25 mm I.D. * 0.25 μm (film thickness dimension; Thermo Fisher Scientific, Waltham, MA, USA). The flow rate control was 1 mL min−1. 11-Hydroxyferruginol, sugiol, and 11-hydroxy sugiol were detected in vitro by UPLC-Qtof-MS (Waters Technologies, Milford, MA, USA). A BEH column (2.1 × 50 mm, 1.8 μm particle size; Waters Technologies, New York, NY, USA) was used for chromatographic separation. The mobile phase was acetonitrile and water (1% formic acid), with a UV absorption wavelength of 254 nm. Carnosic acid was used as an internal standard at 10 μM.
8
Thyme Oil Composition Analysis
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Prior to behavioral assays, the chemical composition of thyme oil was analyzed using a GC (Thermo Finnigan Trace 2000 GC) coupled to a Trace MS quadrupole mass spectrometer (ThermoFisher Scientific, Madrid, Spain) equipped with a TR-5MS capillary column (30 m length, 0.25 mm i.d., film thickness 0.25 mm) (ThermoFisher Scientific). The percentage of p-cymene, carvacrol and thymol was estimated by peak area normalization. Additional compounds with an abundance of ≥ 3% were considered for identification by comparison of their mass spectra with those of reference compounds from NIST mass spectra library ( NIST Chemistry WebBook, NIST Standard Reference Database Number 69), and synthetic standards.
9
Polycyclic Aromatic Hydrocarbon Analysis
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Briey, the MSD was rst test run and standardized with DCM blank (1 mL). Then, 1 mL of the extract was injected into the injection port. A fused silica TR-5ms capillary column (30 m  0.25 mm i.d.) with a lm thickness of 0.25 mm (Thermo Fisher, USA) was used in the column separation. High-purity helium (99.9%) was used as the carrier gas, makeup gas, and purge gas at ow rates of 0.8, 35, and 30 mL min À1 , respectively. The gas chromatogram was operated in split-less mode and separation was conducted with the oven temperature programmed as follows: initial setting at 50 C (2 min hold), ramped to 310 C at 24 C min À1 (for 2 min), and, nally, to 320 C at 5 C min À1 (10 min hold). The injector was held at 250 C and the detector maintained at 340 C. The external standard calibration comprising 17 PAH standards was used to extrapolate the identity and quantity of each component peak in the sample chromatogram. In the integration events, the following conditions were ensured: slope sensitivity (3.7936), peak width (0.4248), area reject (3.1407), height reject (0.4037), and shoulders (off).
10
Floral Volatile Organic Compounds Analysis
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Floral VOC samples were analyzed with a Thermo Trace 1310 GC coupled to a Thermo ISQ MS with electron ionization (EI) at 70.0 eV at 250 °C, using helium as the carrier gas at 1.0 ml min-1 with the injector temperature set at 250 °C. Oven conditions included an initial temperature of 40 °C followed by an immediate ramp of 3 °C min -1 to 200 °C. Available standards, 1 μl of samples, and a continuous series of n-alkanes (C8-C20; Sigma-Aldrich) were injected in the split mode onto a TR-5MS capillary column (30 m × 0.25 mm I.D., film thickness 0.25 μm; Thermo Fisher Scientific). Compounds were identified with retention time matches to pure standards, mass spectra, and/or linear retention indexes calculated with the alkane series (Adams 2007; (link)NIST 2008; El-Sayed 2013) . Standard curves of available compounds were used to calculate final VOC results, which were expressed as ng compound g -1 DW hr -1 .
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