Recombinant human nrg1

Manufactured by Cell Signaling Technology

Sourced in United Kingdom

Recombinant human NRG1 is a protein product produced by Cell Signaling Technology. It is a member of the neuregulin growth factor family, which plays a role in the regulation of cell-cell interactions, neuronal survival, and synaptic function.

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Lab products found in correlation

5 protocols using recombinant human nrg1

Inhibition of Receptor Tyrosine Kinases

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Trametinib (GSK1120212), selumetinib (AZD6244), lapatinib, crizotinib and MK2206 were purchased from Selleck Chemicals (Houston, TX). Recombinant human NRG1, PDGF-BB, and IGF-1 were purchased from Cell Signaling Technology (Beverly, MA); recombinant human EGF was purchased from Lonza Walkersville Inc141 (Walkersville, MD); recombinant human HGF was provided by Michael P. Lisanti (University of Manchester, UK). Humanized ERBB3 monoclonal antibody U3-1287 was provided by U3 Pharma (Martinsried, Germany).

Cheng H., Terai M., Kageyama K., Ozaki S., McCue P.A., Sato T, & Aplin A.E. (2015). Paracrine effect of NRG1 and HGF drives resistance to MEK inhibitors in metastatic uveal melanoma. Cancer research, 75(13), 2737-2748.

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Inhibition of ErbB3 Signaling

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Recombinant human NRG1 was purchased from Cell Signaling Technology (Danvers, MA). Pertuzumab was obtained from the Thomas Jefferson University Pharmacy. huHER3-8, an ErbB3 antagonistic humanized antibody (22 (link)), was produced and purified at ImmunoGen, Inc. The monoclonal antibody, NG33, blocks NRG1 binding to the ErbB3 (25 (link)). Trametinib/GSK’212 and PD0325901 were purchased from Selleck Chemicals LLC (Houston, TX).

Capparelli C., Rosenbaum S., Berman-Booty L.D., Salhi A., Gaborit N., Zhan T., Chervoneva I., Roszik J., Woodman S.E., Davies M.A., Setiady Y.Y., Osman I., Yarden Y, & Aplin A.E. (2015). ErbB3/ErbB2 complexes as a therapeutic target in a subset of wild-type BRAF/NRAS cutaneous melanomas. Cancer research, 75(17), 3554-3567.

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Growth Factor and Inhibitor Procurement

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Recombinant human NRG1 and insulin-like growth factor 1 (IGF1) were purchased from Cell Signaling Technology. Recombinant human epidermal growth factor (EGF), platelet-derived growth factor beta (PDGFbb), and hepatocyte growth factor (HGF) were purchased from Lonza, R&D Systems and Proteintech, respectively. LJM716 was produced and purified by Novartis (Cambridge, MA). Trametinib and PD0325901 were purchased from Selleck Chemicals (Houston, TX).

Capparelli C., Purwin T.J., Heilman S.A., Chervoneva I., McCue P.A., Berger A.C., Davies M.A., Gershenwald J.E., Krepler C, & Aplin A.E. (2018). ErbB3 targeting enhances the effects of MEK inhibitor in wild-type BRAF/NRAS melanoma. Cancer research, 78(19), 5680-5693.

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Investigating NRG1-induced Signaling Pathways in Primary Trophoblasts

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To investigate the activation of signalling mediators, overnight-cultivated primary trophoblasts were serum-starved for 4 h and stimulated with recombinant human NRG1 (20 ng/ml, Cell Signaling Technology) or DSC-conditioned medium for 5 and 20 min. When indicated, ErbB2 and ErbB3 single, or ErbB2–ErbB3 double knockdown was performed, or cells were pre-incubated with an anti-ErbB3 monoclonal antibody (10 µg/ml; R&D Systems) for 30 min. A citrate solution (4 µM) and an IgG mAb (10 µg/ml; Cell Signaling Technology) were used as vehicle control. Cells were lysed in Laemmli buffer and subjected to western blotting. Samples for the PathScan^® RTK Signaling Antibody Array Kit (Cell Signaling Technology) were processed according to the manufacturer's instructions. Signals were detected with chemiluminescence, and pixel intensities were determined by densitometry using Alpha View 3.1.1.0 software (Alpha Innotech, San Leandro, CA).

Fock V., Plessl K., Draxler P., Otti G.R., Fiala C., Knöfler M, & Pollheimer J. (2015). Neuregulin-1-mediated ErbB2–ErbB3 signalling protects human trophoblasts against apoptosis to preserve differentiation. Journal of Cell Science, 128(23), 4306-4316.

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Signaling Pathway Modulation in Cell Studies

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Recombinant human NRG1 was purchased from Cell Signaling Technology. Dabrafenib, trametinib, and JQ1 were purchased from Selleck Chemicals LLC (Houston, TX). PLX51107 was a gift from Plexxikon Inc. (Berkeley, CA).

Tiago M., Capparelli C., Erkes D.A., Purwin T.J., Heilman S.A., Berger A.C., Davies M.A, & Aplin A.E. (2020). Targeting BRD/BET proteins inhibits adaptive kinome upregulation and enhances the effects of BRAF/MEK inhibitors in melanoma. British Journal of Cancer, 122(6), 789-800.

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