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Np aecm ficoll

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NP-AECM-Ficoll is a protein purification resin designed for the separation and purification of biomolecules. It is composed of a Ficoll polymer matrix that is modified with N-acryloylamino-ethoxyethyl-carbamoyl-methyl groups, which provide ion exchange properties for the capture and separation of target proteins and other biomolecules.

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Lab products found in correlation

Np lps, by Biosearch Technologies (2 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions) Addavax, by InvivoGen (1 mentions) Ovalbumin (ova), by InvivoGen (1 mentions) Panitumumab, by Amgen (1 mentions) C57bl 6ncrl, by Charles River Laboratories (1 mentions) Bevacizumab, by Roche (1 mentions)

Spelling variants of this product

NP-Ficoll (25 citations) NP(40)-AECM-Ficoll (3 citations) Ficoll (2 citations) NP(49)-AECM-Ficoll (2 citations)

5 protocols using np aecm ficoll

1

Induction of T-Dependent and T-Independent Immune Responses

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For T-dependent immune responses, mice were immunized i.p. with 1×109 SRBCs in PBS. For T-independent type-I and type-II responses, mice were immunized i.p. with 20 μg NP-LPS or 30 μg of NP-AECM-FICOLL (both Biosearch Technologies), respectively. Peripheral blood and spleens were removed at the indicated time-points for analysis.
Milanovic M., Heise N., De Silva N.S., Anderson M.M., Silva K., Carette A., Orelli F., Bhagat G, & Klein U. (2016). Differential requirements for the canonical NF-κB transcription factors c-REL and RELA during the generation and activation of mature B-cells. Immunology and cell biology, 95(3), 261-271.
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2

Immunization Protocol with Ovalbumin and Adjuvants

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All animal experiments were performed in accordance with the Federal and Cantonal laws of Switzerland and approved by the Cantonal Veterinary Office of Basel-Stadt. Female mice C57Bl/6NCrl were obtained from Charles River, France. All mice were used at an age of 7–11 weeks.
Mice were immunized 3 times once a week sub-cutaneously in the neck with 50 μg Ova (Invivogen) together with adjuvant AddaVax™ (MF59, Invivogen) at 1:5 v/v. NP-OvaL and NP-AECM-Ficoll (Biosearch Technologies) were injected at 50 μg/ mouse. Eventually the following compounds were co-injected as indicated: IVIg (Octagam 10%; Octapharma), bevacizumab (Roche) or panitumumab (Amgen). All injections volumes were completed to 600 μL with DPBS (Gibco Invitrogen). Mice were terminated 24h after the last injection and blood and organs were collected.
Sordé L., Spindeldreher S., Palmer E, & Karle A. (2017). Massive immune response against IVIg interferes with response against other antigens in mice: A new mode of action?. PLoS ONE, 12(10), e0186046.
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3

Induction of T-Dependent and T-Independent Immune Responses

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For T-dependent immune responses, mice were immunized i.p. with 1×109 SRBCs in PBS. For T-independent type-I and type-II responses, mice were immunized i.p. with 20 μg NP-LPS or 30 μg of NP-AECM-FICOLL (both Biosearch Technologies), respectively. Peripheral blood and spleens were removed at the indicated time-points for analysis.
Milanovic M., Heise N., De Silva N.S., Anderson M.M., Silva K., Carette A., Orelli F., Bhagat G, & Klein U. (2016). Differential requirements for the canonical NF-κB transcription factors c-REL and RELA during the generation and activation of mature B-cells. Immunology and cell biology, 95(3), 261-271.
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4

Humoral Immune Response to rSFV-β-gal and NP-Ficoll

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On day 0, each mouse was i.p. immunized with 2 × 106 infectious units (IU) of rSFV vector encoding β-galactosidase (rSFV-β-gal). On day 7, each mouse was further i.p. immunized with 40 μg of NP-AECM-Ficoll (Biosearch Technologies). On day 13, blood from the sub-mandibular vein of mice was collected, and the T-dependent antibody to rSFV-β-gal (β-galactosidase specific IgG) and the T-independent antibody to NP-Ficoll (NP specific IgM) in the serum were measured by ELISA.
Zhang D., Yue T., Choi J.H., Nair-Gill E., Zhong X., Wang K.W., Zhan X., Li X., Choi M., Tang M., Quan J., Hildebrand S., Moresco E.M, & Beutler B. (2019). Syndromic immune disorder caused by a viable hypomorphic allele of spliceosome component Snrnp40. Nature immunology, 20(10), 1322-1334.
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5

Immunization and Antibody Response Assay

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Immunization and ELISA was performed as previously described (50 (link)). On day 0, mice were i.p. immunized with either 2×106 infectious units (IU) of rSFV vector encoding β-galactosidase (rSFV-β-gal) or 40 μg of NP-AECM-Ficoll (Biosearch Technologies). On days 7 and 14, blood was collected for analysis of the antibody responses to NP-Ficoll (NP-specific IgM) and rSFV-β-gal (β-galactosidase-specific IgG) by ELISA.
ELISA analysis of IL-2 was performed according to the manufacturer’s instructions (#88–7024-22, eBioscience).
Yue T., Zhan X., Zhang D., Jain R., Wang K.W., Choi J.H., Misawa T., Su L., Quan J., Hildebrand S., Xu D., Li X., Turer E., Sun L., Moresco E.M, & Beutler B. (2021). SLFN2 protection of tRNAs from stress-induced cleavage is essential for T cell-mediated immunity. Science (New York, N.Y.), 372(6543), eaba4220.
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