The cell lines (MDA-MB-231, MDA-MB-468, MDA-MB-436, MCF-10A, MCF-7, BT-474, and SK-BR3) were acquired from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and Procell (Wuhan, China). All cell lines except for MDA-MB-468 were cultured in a humidified incubator at 37°C with 5% CO2. MDA-MB-231 and MDA-MB-436 cells were grown in DMEM supplemented with 10% FBS (Procell, Wuhan, China) and 1% penicillin/streptomycin (Yeasen, Shanghai, China). MCF-10A, BT-474, and SK-BR3 cells were grown in appropriate special complete medium. MCF-7 cells were cultured in 1640 medium enriched with 10% FBS (Procell) and 1% penicillin/streptomycin (Yeasen). MDA-MB-468 cells were cultured in L-15 medium with 10% FBS (Procell) and 1% penicillin/streptomycin (Yeasen) in a humidified incubator at 37°C without CO2.
Penicillin streptomycin
Manufactured by Yeasen
Sourced in China, United States
Penicillin/streptomycin is a combination of two antibiotics commonly used in cell culture applications. It serves as an antimicrobial agent to prevent bacterial contamination in various laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (11 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (7 mentions)
Rpmi 1640, by Thermo Fisher Scientific (6 mentions)
Fetal bovine serum (fbs), by Yeasen (5 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
B27 supplement, by Thermo Fisher Scientific (2 mentions)
N2 supplement, by Thermo Fisher Scientific (2 mentions)
Glutamax supplement, by Thermo Fisher Scientific (2 mentions)
Mem non essential amino acids solution, by Thermo Fisher Scientific (2 mentions)
Cmrl 1066, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Corning (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Mda mb 436, by Procell (1 mentions)
Fetal bovine serum (fbs), by Procell (1 mentions)
Mda mb 231, by Procell (1 mentions)
Bt 474, by Procell (1 mentions)
Mcf 10a, by Procell (1 mentions)
Mda mb 468, by Procell (1 mentions)
Mcf 7, by Procell (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
35 protocols using penicillin streptomycin
1
Culturing Diverse Breast Cell Lines
2
Breast Cancer Cell Culture Protocol
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Human normal breast epithelial cells MCF-10A and BC cell lines (MCF7, BT474, SKBr-3, ZR-75-30, MDA-MB-453 and MDA-MB-231) were bought from ATCC (Rockville, USA). These cells were grown in the RPMI-1640 complete medium (Thermo Scientific Hyclone, Utah, USA) which contained 10% fetal bovine serum (FBS, Thermo Scientific Hyclone, Utah, USA) and 1% penicillin/streptomycin (Yeasen Biotech Co., Ltd., Shanghai, China) and kept at 37°C with 5% CO2 and saturated humidity. The medium was replaced every 2 to 3 days. When the cells got 90% confluency, 0.25% trypsin (Thermo Scientific Hyclone, Utah, USA) was adopted for cell trypsinization and subculture.
3
Human and Mouse Lung Cancer Cell Lines
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The cell lines used in this study included the human NSCLC cell lines A549, PC-9, NCI-H460, NCI-H1299, NCI-H1703 and human bronchial epithelial (HBE) cells. The mouse lung cancer cell lines LLC and HEK-293 T were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cells were cultivated in DMEM and RPMI-1640 (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco, USA) and 1% penicillin–streptomycin (Yeasen, Shanghai, China). The environmental conditions were 37 °C and 5% CO2.
4
Nanogel Synthesis and Characterization
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GOx nanogel (GN) and GOx-CAT nanogel (GCN) were synthetized in our previous study(Luo et al., 2021b (link); Fan et al., 2022 (link)). Trypsin-EDTA (ethylenediaminetetraacetic acid) was obtained from Beyotime Biotechnology. Dimethyl sulfoxide (DMSO) was supplied by Sinopharm Chemical Reagent Co. Ltd. Phosphate buffered saline (PBS) and bovine serum albumin (BSA) were supplied by Solarbio Technology Co., Ltd. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), penicillin-streptomycin, hematoxylin eosin (H&E staining kit), calcein-AM/PI double stain kit, and antifade mounting medium with DAPI (4ʹ,6-diamidino-2-phenylindole) were obtained from Yeasen Biotechnology Co. Ltd. Anti-fluorescence quenching tablet seal (including DAPI) was obtained from Shandong Sparkjade Biotechnology Co., Ltd. Fetal bovine serum (FBS) was obtained from Thermo Fisher Scientific Co., Ltd. A blood glucose meter was supplied by Sinocare Inc. A JPB-607A portable dissolved oxygen tester and a PHS-3C pH meter were supplied by Shanghai INESA Scientific Instrument Co., Ltd. The instruments used in the experiments were all from the Core Facility of Biomedical Sciences, Xiamen University.
5
Culturing Human Gastric Carcinoma and HEK293FT Cells
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Human gastric carcinoma AGS-1, HGC-27, and HEK293FT cells were purchased from the Cell Bank of the Chinese Academy of Science. The AGS-1, HGC-27, and HEK293FT cells were cultured with RPMI-1640 (Gibco, Waltham, MA, USA, 11875101) or DMEM (Gibco, 11965092) supplemented with 10% fetal bovine serum (Gibco, 10099) and 1% penicillin–streptomycin (100×) (Yeasen, Shanghai, China, 60162ES76).
6
Isoproterenol-Induced Hypertrophy in H9c2 Cells
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The H9c2 cells, derived from embryonic rat myocardial tissue, were used in this study to induce hypertrophy [37 (link)–39 (link)]. H9c2 cells (GNR 5) were obtained from the Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). The cells were cultured in 24-well plates at 37 ℃ in a humidified environment with 5% CO2 using Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Gibco, Cat#10099–141) and 1% penicillin/streptomycin (Yeasen, Cat#60162ES76). To induce cardiomyocyte hypertrophic injury, H9c2 cells were treated with β-adrenergic agonist, isoproterenol (ISO), as described previously [40 (link)–42 (link)]. Briefly, cells were cultured with serum-free DMEM medium for 12 h and then exposed to 10, 30, and 60 μM ISO (MCE, Cat#HY-B0468) or vehicle (dimethyl sulfoxide, DMSO) for 24 h. The collected cells were subjected to qPCR analysis.
7
Expansion and Characterization of R28 Retinal Precursor Cells
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R28 cells, a retinal precursor cell line and have the potential for differentiations, could express specific antigen Thy 1.1 of RGCs15 (link), and are commonly used to study neuronal function and neuroprotection in vitro16 (link), which were obtained from the Department of Anatomy and Neurobiology of Central South University (Changsha, China). Cells were expanded as previously reported17 (link) and cultured in low glucose Dulbecco’s modified Eagle’s medium (DMEM; Hyclone, UT, USA) supplemented with 10% fetal calf serum (Gibco, Carlsbad, CA) and 1% penicillin-streptomycin (Yeasen, Shanghai, China). Cells were maintained in a humidified incubator at 37 °C with an atmosphere containing 5% CO2. The cells were regularly tested for mycoplasma contamination using One-step Quickcolor Mycoplasma Detection Kit according to the manufacturer’s instructions (Shanghai, China).
8
Lung Cancer and Lymphoma Cell Culture
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Lewis lung cancer cells were obtained from the Cell Bank of the Chinese Academy of Sciences and maintained in high‐glucose DMEM (SH30243.01; Hyclone) supplemented with 10% foetal bovine serum (FBS; Biowest) and 1% penicillin‐streptomycin (60162ES76; YEASEN). YAC‐1 (ATCC® TIB160™) cells were cultured in RPMI‐1640 medium (SH30809.01B; Hyclone) supplemented with 10% FBS and 1% penicillin‐streptomycin. All cell lines were incubated at 37°C in a humidified atmosphere containing 5% CO2.
9
Collection and Culture of RCC Tissues
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Twenty-five pairs of freshly biopsied RCC tumor tissues and the corresponding adjacent normal kidney tissues were collected from RCC patients who had undergone laparoscopic nephrectomy from the Department of Urology at the Zhongda Hospital from 2019 to 2020. All patients were diagnosed to have RCC and did not receive any anti-tumor therapy before surgery. Ethical approval for this study was obtained from the Ethics Committee and Clinical Research Institutional Review Board of Zhongda Hospital. Informed consent was obtained from all patients or their relatives.
Human RCC cell lines 786-O and Caki-1 and normal renal tubular epithelial cells, HK-2, were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The 786-O and Caki-1 cells were cultured in Dulbecco's modified Eagle's medium, whereas the HK-2 cells were cultured in a keratinocyte medium supplemented with 1% keratinocyte. All cell cultures were supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Yeasen, Shanghai, China). The cells were all incubated at 37°C containing 5% CO 2 . RCC cell lines were stored at -80°C using the instrument CELLSAVING (NCM, Suzhou, China) .
Human RCC cell lines 786-O and Caki-1 and normal renal tubular epithelial cells, HK-2, were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The 786-O and Caki-1 cells were cultured in Dulbecco's modified Eagle's medium, whereas the HK-2 cells were cultured in a keratinocyte medium supplemented with 1% keratinocyte. All cell cultures were supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Yeasen, Shanghai, China). The cells were all incubated at 37°C containing 5% CO 2 . RCC cell lines were stored at -80°C using the instrument CELLSAVING (NCM, Suzhou, China) .
10
Cell Line Cultivation and Genetic Modifications
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J774A.1 and THP-1 cells were obtained from China Infrastructure of Cell Lines Resource (China). The THP-1 cell line with GSDMD knockout (THP-1 GSDMD-KO) was reported previously [7 (link)]. J774A.1 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Corning, NY, USA) supplemented with 10% (v/v) FBS (Sigma-Aldrich, USA) and 1% penicillin-streptomycin (Yeasen, shanghai, China) at 37 °C with 5% CO2. THP-1 and THP-1 GSDMD-KO cells were cultured in RPMI 1640 medium (Gibco, Renfrewshire, UK) supplemented with 10% (v/v) FBS and 1% penicillin and streptomycin. THP-1-Null, THP-1-defCasp1 (Casp1-KD), and THP-1-defNLRP3 (NLRP3-KD) were purchased from InvivoGen and cultured as instructed by the manufacturer.
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