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Anti human 4e bp1

Manufactured by Cell Signaling Technology
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Anti-human 4E-BP1 is a laboratory reagent used to detect and quantify the expression of the 4E-BP1 protein in human samples. 4E-BP1 is a regulator of protein synthesis and plays a role in cellular growth and proliferation.

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Lab products found in correlation

Anti human akt, by Cell Signaling Technology (2 mentions) Supersignal west pico luminol enhancer solution, by Thermo Fisher Scientific (2 mentions) F ab 2 fragment specific, by Jackson ImmunoResearch (2 mentions) Anti human phospho akt ser 473, by Cell Signaling Technology (2 mentions) Anti human phospho 4e bp1 thr37 46, by Cell Signaling Technology (2 mentions) Anti human phospho s6 ribosomal protein ser235 236, by Cell Signaling Technology (2 mentions) Anti human s6 ribosomal protein, by Cell Signaling Technology (2 mentions) Peroxidase affinipure f ab 2 fragment goat anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Asct2, by Cell Signaling Technology (1 mentions) Phospho 4e bp1 ser65, by Cell Signaling Technology (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Phospho eif2α ser51, by Cell Signaling Technology (1 mentions) Mouse anti human β actin antibody, by Merck Group (1 mentions) Rabbit anti human s6k, by Cell Signaling Technology (1 mentions) Phospho s6k thr389, by Cell Signaling Technology (1 mentions) Eif2α 9722, by Cell Signaling Technology (1 mentions) Chemi lumi one l, by Nacalai Tesque (1 mentions) Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions)

Spelling variants of this product

4E-BP1 (409 citations) Anti-4E-BP1 (139 citations) Anti-human phospho-4E-BP1 (Thr37/46), (2 citations)

3 protocols using anti human 4e bp1

1

Quantifying PI3K-AKT-mTOR Signaling

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Example 4

Western Blotting of Components of the PI3K-AKT-mTOR Signaling Pathway

Primary antibodies used to measure PI3K-AKT-mTOR signaling were anti-human phospho-AKT (Ser 473) (clone: 193H12), anti-human AKT, anti-human phospho-4E-BP1 (Thr37/46), anti-human 4E-BP1, anti-human phospho-S6 ribosomal protein (Ser235/236) (clone: 2F9), and anti-human S6 ribosomal protein (clone: 54D2) (Cell Signaling; Beverly, Mass.). Secondary antibodies used were Peroxidase-AffiniPure F(ab′)2 Fragment Goat anti-Rabbit IgG, F(ab′)2 Fragment Specific, and Peroxidase-AffiniPure F(ab′)2 Fragment Goat anti-Mouse IgG, Fcγ Fragment Specific (Jackson ImmunoResearch, West Grove, Pa.). Western blots were developed using SuperSignal West Pico Luminol Enhancer Solution (Thermo Scientific; Rockford, Ill.) chemiluminescent substrate and visualized following exposure to film.

US11607460B2. Synergistic effects between auristatin-based antibody drug conjugates and inhibitors of the PI3K-AKT mTOR pathway (2023-03-21). SEATTLE GENETICS, INC. [US]. Inventors: Timothy S. Lewis [US], Che-Leung Law [US], Julie A. McEarchern [US].
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2

PI3K-AKT-mTOR Pathway Profiling

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Example 4

Western Blotting of Components of the PI3K-AKT-mTOR Signaling Pathway

Primary antibodies used to measure PI3K-AKT-mTOR signaling were anti-human phospho-AKT (Ser 473) (clone: 193H12), anti-human AKT, anti-human phospho-4E-BP1 (Thr37/46), anti-human 4E-BP1, anti-human phospho-S6 ribosomal protein (Ser235/236) (clone: 2F9), and anti-human S6 ribosomal protein (clone: 54D2) (Cell Signaling; Beverly, Mass.). Secondary antibodies used were Peroxidase-AffiniPure F(ab′)2 Fragment Goat anti-Rabbit IgG, F(ab′)2 Fragment Specific, and Peroxidase-AffiniPure F(ab′)2 Fragment Goat anti-Mouse IgG, Fcγ Fragment Specific (Jackson ImmunoResearch, West Grove, Pa.). Western blots were developed using SuperSignal West Pico Luminol Enhancer Solution (Thermo Scientific; Rockford, Ill.) chemiluminescent substrate and visualized following exposure to film.

US10201615B2. Synergistic effects between auristatin-based antibody drug conjugates and inhibitors of the PI3K-AKT mTOR pathway (2019-02-12). Seattle Genetics, Inc. [US]. Inventors: Timothy S. Lewis [US], Che-Leung Law [US], Julie A. McEarchern [US].
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3

Protein Expression Analysis by Western Blot

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Cells were lysed with lysis buffer containing 50 mM Tris-HCl, 1% sodium dodecyl sulfate, 1 mM dithiothreitol and 0.43 mM 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride. The lysates were sonicated and centrifuged at 20,400× g at 4 °C for 20 min, and the supernatant was collected. Equal amounts of the protein extract were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride membrane (EMD Millipore, Billerica, MA, USA). The following were used as the primary antibodies: rabbit anti-human S6K (#2708), phospho-S6K (Thr389) (#9234), anti-human 4E-BP1 (#9644), phospho-4E-BP1 (Ser65) (#9451), ATF4 (#11815), ASCT2 (#8057), phospho-eIF2α (Ser51) (#9721), eIF2α (#9722) antibodies (Cell Signaling Technology, Inc., Danvers, MA, USA), mouse anti-human β-actin antibody (A5441; Sigma), mouse anti-human ASNS antibody (G-10; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-human GPT2 (16757-1-AP) antibody (Proteintech Group, Inc., Chicago, IL, USA), rabbit anti-human Glutaminase (ab156876) and xCT(ab37185) antibodies (Abcam, Cambridge, UK). Signals were detected with Chemi-Lumi One L (Nacalai Tesque, Kyoto, Japan) or Immobilon Western Chemiluminescent HRP Substrate (EMD Millipore). All experiments shown were replicated at least twice.
Boku S., Watanabe M., Sukeno M., Yaoi T., Hirota K., Iizuka-Ohashi M., Itoh K, & Sakai T. (2020). Deactivation of Glutaminolysis Sensitizes PIK3CA-Mutated Colorectal Cancer Cells to Aspirin-Induced Growth Inhibition. Cancers, 12(5), 1097.
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