3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide tetrazolium mtt

All cell culture reagents were purchased from Gibco-Invitrogen (Carlsbard, CA, USA). Cell culture dishes and other plasticware were obtained from Nalgene Nunc (Rochester, NY, USA). Salts and buffers were purchased Sigma (St. Louis, MO, USA). Leptomycin B (LMB), α-tocopherol, LPS O111:B4 and (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium (MTT) were obtained from Sigma-Aldrich. Primary antibodies used in protein detection of CALM, α-adaptin, β-adaptin, eps 15, c-Myc, c-Jun, c-Abl and SR-B1 as well as secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and Abcam (Cambridge, UK). Fluorescent conjugates of LPS BODIPY FL, and 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate for ROS detection were obtained from Thermo Fisher Scientific (Waltham, MA, USA).

Potassium hydroxide (KOH) was obtained from Merck (Darmstadt, Germany). Hydrochloric acid (HCl) was purchased from Sigma-Aldrich (Rio de Janeiro, Brazil). Ferric dichloride tetrahydrate (FeCl₂•4H₂O) (reagent grade, p.a., ≥99%) was obtained from J.T. Baker, Inc. (Phillipsburg, NJ, USA). Ferric trichloride hexahydrate (FeCl₃•6H₂O) (reagent grade, p.a., ≥97%) and Tetraethylammonium hydroxide ((C₂H₅)₄N(OH)) as an aqueous solution 20% wt) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). All reagents were used as given without further purification. These materials were used for the reference synthesis [45 (link)]. Commercial turmeric powder from India (TRS, Curcuma longa L. Powder) was used in this study. Deionized water and ethanol were used in both experiments.
Chemicals such as dimethyl sulfoxide (DMSO) (Cat. D2650; 100% purity), Neutral red (NR), and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide tetrazolium (MTT) were obtained from Sigma Chemical Co. (St. Louis, MO, USA); RPMI-1640 and DMEM medium, Fetal bovine serum (FBS), phytohemagglutinin, nonessential amino acids (100 mM), L-glutamine, sodium pyruvate, and antimycotic solutions were obtained from Invitrogen-Gibco (Carlsbad, CA, USA). Other reagents were obtained from J.T. Baker, Inc. (Deventer, the Netherlands), as indicated.