Ultima flo m permafluor e

[¹⁴C]-labeled TBBPA (ring-labeled; Figure 1, Lot # 3225-235, Perkin Elmer Life and Analytical Sciences [Boston, MA], re-purified in 2013 by Moravek Biochemicals [Brea, CA]) and used in these studies had a radiochemical purity of >98% (specific activity = 90.3 mCi/mmol) and a relative chemical purity of >98%, as compared to a TBBPA reference standard (Sigma-Aldrich; St. Louis, MO). Scintillation cocktails were obtained from MP Biomedicals (Ecolume; Santa Ana, CA) or Perkin-Elmer (Ultima-Flo M & PermaFluor E+; Torrance, CA). All other reagents used in these studies were high performance liquid chromatography (HPLC) or analytical grade.

[¹⁴C]-radiolabeled TBBPA (uniform ring-labeled; Figure 1), previously described by Kuester et al (2007) (link) was generously provided by I. Glenn Sipes at the University of Arizona (Tucson, AZ) for use in the present study. Reversed-phase HPLC fractionation (described below) was used to acquire a radiochemical purity of >98% (specific activity = 90.3 mCi/mmol). [¹⁴C]-TBBPA was reconstituted in dimethyl sulfoxide. Chemical purity was determined to be >98% as compared to a TBBPA reference standard (Sigma-Aldrich; St. Louis, MO). Scintillation cocktails were obtained from MP Biomedicals (Ecolume; Santa Ana, CA) or Perkin-Elmer (Ultima-Flo M & PermaFluor E+; Torrance, CA). Cremophore EL®, dimethylsulfoxide, β-glucuronidase (EC 3.2.1.31, Type B-10), ammonium acetate, D-saccharic acid 1, 4-lactone, and aryl sulfatase were purchased from Sigma-Aldrich. All other reagents used in these studies were high performance liquid chromatography (HPLC) or analytical grade.