The marigold (C. officinalis L.) flowers (2 kg) were dried in shade, and extracted with neat methanol several times till complete exhaustion. The methanol in the combined extracts was distilled off at 50 °C in a rotary evaporator. The remaining concentrated extract was then subjected to preparative TLC using the BAW solvent system butanol: acetic acid: water (4:1:5, upper layer). A major band showing a dark purple color upon using UV lamp at 365 nm and quenched the UV light at 254 nm, was scratched, dissolved in methanol and the solvent was evaporated in vacuo at 50 °C, to obtain a yellow powder that was subjected to UV and NMR spectroscopic analysis. 1H and 13C-NMR spectroscopic data were measured at 400 and 100 MHz, respectively, in DMSO on a Bruker Ascend-400 spectrometer (Avance BioSpin Inc., Rheinstetten, Germany). The chemical shifts (recorded in ppm) were measured using tetramethyl silane (TMS) as internal standard. The UV spectroscopic measurement was performed on UV-Spectrophotometer (Jasco V630, Tokyo, Japan).
Uv spectrophotometer
Manufactured by Jasco
Sourced in Japan
A UV spectrophotometer is an analytical instrument used to measure the absorbance or transmittance of light by a sample in the ultraviolet and visible regions of the electromagnetic spectrum. It is primarily used for quantitative chemical analysis and identification of unknown substances.
Automatically generated - may contain errors
Lab products found in correlation
Varioskan microplate reader, by Thermo Fisher Scientific (1 mentions)
Whatman, by Cytiva (1 mentions)
Phenothiazine, by Merck Group (1 mentions)
1 bromohexane, by Merck Group (1 mentions)
Kieselgel 60 f254, by Merck Group (1 mentions)
Dmx 400 spectrometer, by Bruker (1 mentions)
Sulfadiazine, by Merck Group (1 mentions)
Sulfathiazole, by Merck Group (1 mentions)
Fluorimeter 8300, by Jasco (1 mentions)
Spectrum 400, by PerkinElmer (1 mentions)
11 protocols using uv spectrophotometer
1
Isolation and Characterization of Marigold Flavonoids
2
M2-1-tRNA70 Condensation Dynamics
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Turbidity was used as a qualitative parameter of condensation. Measurements were made at 370 nm using a Thermo Fisher Scientific Varioskan micro plate reader (Thermo Fisher Scientific, Waltham, MA, USA) for the microscopy experiments. All the conditions were measured in 96-well plates (Corning nonbinding surface).
Complexes formed by M2-1-tRNA70 condensation were recorded in a Jasco UV spectrophotometer (Jasco, Hachioji, Tokyo, Japan) by following absorbance signals at 370 nm at 20 °C. Measurements were performed in 50 mM HEPES pH 7.5, 75 mM NaCl, and 5% PEG-4000. Addition of NaCl up to a concentration of 0.5 M was made after absorbance reached maximum signal.
Complexes formed by M2-1-tRNA70 condensation were recorded in a Jasco UV spectrophotometer (Jasco, Hachioji, Tokyo, Japan) by following absorbance signals at 370 nm at 20 °C. Measurements were performed in 50 mM HEPES pH 7.5, 75 mM NaCl, and 5% PEG-4000. Addition of NaCl up to a concentration of 0.5 M was made after absorbance reached maximum signal.
3
Quantifying Encapsulation Efficiency of DFZ in UENVs
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Percent EE of DFZ in the prepared UENVs was detected by measuring the amount of free DFZ in the dispersion medium.17 (link) By centrifugation of 1 mL of each freshly prepared UENVs suspension using cooling centrifuge (SIGMA 3–30K, Germany) at 14,000 rpm at 4°C for 3 h to ensure complete settling of UENVs, the resultant supernatant was separated, then was appropriately diluted with 10% methanolic phosphate buffer solution (pH 5.5), filtered through 0.45 µm Millipore polycarbonate membrane filter (Whatman Ltd, Springfield Mill, UK) and analyzed for DFZ concentration spectrophotometrically at 248 nm using a UV spectrophotometer (JASCO, Japan). From the mean of three determinations, %EE was calculated using equation 1:
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$${\rm{\%\ EE }} = {{{\rm{Total\,amount\,of\,DFZ}} - {\rm{ free\,DFZ}}} \over {{\rm{Total\,drug\,of\,DFZ}}}} \times 100$$
\end{document}
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$${\rm{\%\ EE }} = {{{\rm{Total\,amount\,of\,DFZ}} - {\rm{ free\,DFZ}}} \over {{\rm{Total\,drug\,of\,DFZ}}}} \times 100$$
\end{document}
4
UV Spectrophotometric Measurements Protocol
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The measurments were done using UV- spectrophotometer (Jasco; Japan).
5
Synthesis of Novel Phenothiazine Derivatives
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Phenothiazine (98%), 1-bromo hexane, sulfathiazole and sulfadiazine were purchased from Sigma-Aldrich Germany. The solvents used in this study were analytical grade and used without any further purifications such as DMF, acetone, ethanol, methanol, 1,4-dioxane, acetonitrile and n-hexane. Thin layer chromatography (TLC) (Merck, DC Kiesel gel 60 F254) plates were used for monitoring the consumptions of reactant and the forming of the new product. NMR spectra was recorded on a Bruker DMX-400 spectrometer operating at 400, 101 MHz using DMSO-d 6 as solvent. LC/MS with ESI electrospray ionization source (positive ion mode) was investigated using a XEVO TQD triple quadrupole mass spectrometer. UV/vis absorption spectra were measured on a Shimadzu UV spectrophotometer and emission spectra was investigated using a JASCO fluorimeter (8300).
6
Spectroscopic Analysis of Plant Extracts
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The UV spectra of the ethanol extracts of the aerial and root were recorded on a Jasco UV spectrophotometer at a wavelength range of 200–600 nm with a scan speed of 400 nm/min. The spectra used in obtaining the structural properties of the selected plant extract were obtained from the Fourier-transform infrared spectrometer equipped with an attenuated total reflectance (ATR-FTIR), model PerkinElmer Spectrum 400. In the ATR-FTIR method, the sample to be analyzed is placed directly into the sample cell, where a good and reproducible contact between the sample and the crystal of reflection is obtained nondestructively, producing good-quality infrared spectra. The FTIR spectra were recorded in the range of 4000–700 cm−1.
7
Evaluating Pd complex stability
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The different Pd complexes 4a, 4b and 5a were dissolved at 10 mg mL -1 in different solvents and aqueous solutions ( phosphate buffer, water containing Triton X-100, SDS or acetonitrile as co-solvent) and their UV absorbance was measured in a UV spectrophotometer JASCO in the range of 340 to 600 nm. Stability of the complex in aqueous solution was determined by measuring the conservation of the absorbance (ΔAbs) intensity.
8
Quantification of Etexilate in Powdered SAs
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Powdered SAs containing 50 mg ETX was weighed and solubilized in buffer (pH 1.2). Subsequently, the solutions were filtered (0.45µm, Millipore) and suitably diluted to
determine the drug content with UV spectrophotometer at 233 nm (Jasco UV spectrophotometer) using buffer (pH 1.2) as blank. From the absorbance, drug content of the SAs was calculated using equation 1.
determine the drug content with UV spectrophotometer at 233 nm (Jasco UV spectrophotometer) using buffer (pH 1.2) as blank. From the absorbance, drug content of the SAs was calculated using equation 1.
9
Ethion Characterization and Analysis
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Ethion was purified from commercial sources and compared with authentic sample. The Rf values of ethion in different solvent systems (Toluene: Xylene 20: 20; Dioxane: Xylene: Petroleum ether 10: 20: 20 and n-Hexane: Ethyl acetate 98: 2) are 0.75, 0.8 and 0.76, respectively. The absorbance of ethion was measured by UV spectrophotometer (JASCO) at λ=290 nm; the structure of ethion is shown in Figure 1.
10
Spectroscopic Analysis of Meropenem and Cefixime
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The solution of the meropenem, cefixime and its synthesized complexes with metal ions were prepared in methanol (10 µg/ml) and was scanned in the region of 200-600 nm using 630 Jasco UV spectrophotometer.
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