A total of 56 K18‐hACE2 transgenic mice of both genders (C57BL/6 strain) were procured from GemPharmatech Co, Ltd. Additionally, 60 male BALB/c mice aged 5–6 weeks, reared in a specific‐pathogen‐free (SPF) environment, were obtained from Guangdong Medical Laboratory Animal Center. The number of mice used for the C57BL/6 and BALB/c strains is indicated in the figure legend. All mice were raised and vaccinated in the SPF facilities at the Laboratory Animal Center of Sun Yat‐Sen University. Two male and two female adult Rhesus Macaques between 2 and 4 years old were purchased previously from Guangdong Landau Biotechnology Co, Ltd. Monkeys experiments were conducted according to the guidelines and regulations of the Laboratory Monitoring Committee of Guangdong Province of China.
Balb c mice
Manufactured by GemPharmatech
Sourced in China
BALB/c mice are an inbred strain of laboratory mice commonly used in biomedical research. They are a popular model organism for studying immune system function, cancer, and other diseases. The BALB/c strain is characterized by its albino appearance and susceptibility to certain infections and diseases.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6jgpt mice, by GemPharmatech (1 mentions)
Balb c nude mice, by GemPharmatech (1 mentions)
Lentivirus, by Genechem (1 mentions)
Recombinant murine il 33, by Thermo Fisher Scientific (1 mentions)
Concanavalin a (cona), by Merck Group (1 mentions)
Protein g column, by Thermo Fisher Scientific (1 mentions)
C57bl 6j, by GemPharmatech (1 mentions)
Nod scid mice, by Beijing Huafukang Biotechnology (1 mentions)
Balb c nude mice, by Beijing Huafukang Biotechnology (1 mentions)
24 protocols using balb c mice
1
In Vivo SARS-CoV-2 Infection Model
2
Antitumor Effects of Gallic Acid in BALB/c Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five- to six-week-old BALB/c mice were purchased from Gempharmatech Co., Ltd (Nanjing, China) and were maintained in the Animal Experimental Center of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST). CT26 cells (approximately 2 × 105) were subcutaneously injected into the right flanks of BALB/c mice. On day 7 after post-tumor implantation, the mice were randomly divided into three groups (six in each group): a control group, a low-dose group, and a high-dose group. In the low-dose group and high-dose group, 2 and 8 mg/kg GA were intravenously administered via the tail vein every two days, and the control group mice were treated with vehicle (5% DMSO + 95% L-arginine solution (0.53 g/L); i.v.). The body weights and tumor volumes were recorded every two days. On the 11th day, the mice were sacrificed 48 h after the last administration, and blood samples were collected. Then, the tumor and major organs were harvested, weighed, and analysed via H&E, immunohistochemistry, western blotting, and flow cytometry. The tumor volume was calculated based on the following formula: Tumor volume = length × width2 × 0.5.
3
Gut-Brain Axis Modulation in BALB/c Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 8-week-old male BALB/c mice were purchased from Gempharmatech Co., Ltd., (Nanjing, China), and raised in a specified pathogen free (SPF) environment with constant temperature (24 ± 1 °C) and humidity (50 ± 10%), and a 12 h light-dark cycle lighting system for the two-week adaptation period. The forty mice were then randomly divided into four groups (n = 10): control, model, WMP and rapamycin (Rap) group (Figure 1 A). The total experimental period was 8 weeks. The body weight was recorded once a week. After fasting for 12 h, the mice were sacrificed and the brain and the colonic content were quickly removed and quenched in liquid nitrogen and stored at −80 °C for the following analysis.
All experimental operations are carried out following the measures for the Administration of Experimental Animals of Jiangnan University, and all animal ethics are approved by the Committee of Experimental Animal Welfare Ethics of Jiangnan University (JN.No20220530b0960905).
All experimental operations are carried out following the measures for the Administration of Experimental Animals of Jiangnan University, and all animal ethics are approved by the Committee of Experimental Animal Welfare Ethics of Jiangnan University (JN.No20220530b0960905).
4
Evaluating CAG Therapy in Murine Tumor Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Six-eight-week-old female C57BL/6JGpt mice, BALB/c mice and BALB/c nude mice were purchased from GemPharmatech (Nanjing, China). MC38 or CT26 cancer cells (1×106) were inoculated subcutaneously into each mouse. When the tumor grows to 100 mm3, the mice were randomly divided into phosphate buffered saline (PBS) group (ig, once a day) and CAG group (ig, 50 mg/kg, once a day). Tumor volumes were determined by caliper measurement using the formula V=length×width2/2. When the tumor volume of PBS group mice reached 1000 mm3, the tumor of mice was taken out, photographed and weighed.
5
Investigating Tumor Growth and Metastasis in Athymic Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five‐week‐old male athymic BALB/c mice were purchased from GemPharmatech (Nanjing, China) and maintained under specific pathogen‐free conditions. For the in vivo cell proliferation assay, A549 and SPCA1 cells were stably transfected with sh‐Ctrl and sh‐KIAA1429 using lentivirus (GeneChem, Shanghai, China). The cells were subcutaneously injected into either side of the posterior flanks of the mouse. The tumor volume was measured every few days (length×width2×0.5). At the end of the experiment, the mice were euthanized, and the tumors were removed, weighed, and then fixed for hematoxylin and eosin (H&E), BTG2 and Ki‐67 immunostaining analysis. To observe tumor metastasis in the lungs, treated SPCA1 cells were injected into the mice's tail vein. After 60 days, the mice were euthanized, and the lungs were removed. Images of the lungs were taken for recording and used for H&E immunostaining analysis, and the nodules on the lung were counted. All animal care procedures and experiments were conducted following animal welfare guidelines and based on the “3Rs” principle (reduce, replace, refine). This study was conducted in strict accordance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. Our protocol was approved by the Committee on the Ethics of Animal Experiments of Nanjing Medical University.
6
Evaluating IL-33's Impact on 5-FU Chemotherapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To evaluate the effect of IL-33 on 5-FU chemotherapy, 20 female BALB/c mice (4 weeks of age) were purchased from Gempharmatech Co., Ltd (Jiangsu, China). CT26 cells (3 × 105 cells in 100 μL PBS) were injected subcutaneously into 20 BALB/c mice. After 7 days, the tumor size reached about 50 mm3, and the mice were randomly divided into four groups (n = 5 per group). The mice were treated with recombinant murine IL-33 (100 μg/kg intraperitoneal injection once every other day; Peprotech) from days 0 to 17. After 3 days, the mice were or were not treated with 5-FU (10 mg/kg/day intraperitoneal injection; Sigma-Aldrich) from days 3 to 17. Tumor size was measured every 4 days. Mice were sacrificed on day 21, and the tumor volume was calculated using the following formula: (length × width2)/2. Mice were sacrificed when the diameter of the tumor reached 20 mm in any dimension or 60 days after the first recombinant murine IL-33 treatment. Tumors were harvested and subjected to qPCR analysis to determine IL-33, CXCL10, and CXCL13 expression levels and IHC staining to assess CD3, CD206, and cleaved-caspase 3 expression.
7
Moscatilin Protects Against ConA-Induced Liver Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Balb/c mice (Male, 20 and 25 g, 7–9 weeks old) were acquired from GemPharmatech (Nanjing, China). All animal experiments were performed in compliance with protocols approved by Taian City Central Hospital. Moscatilin was solubilized in dimethyl sulfoxide. Before injection, moscatilin stock solution was dissolved in cremophor EL/ethanol and diluted in a 5% glucose solution. Moscatilin in various doses was intragastrically injected daily (days 0, 1, and 2). ConA (Merck Millipore) dissolved in saline was injected through the tail vein at the dose of 20 mg/kg on day 2 to induce liver injury [22 (link)]. For IL-37 blocking studies, mice were treated with both moscatilin and IL-37 neutralizing antibodies (R&D systems, Minneapolis, MN, USA, i.v., 0.4 g/kg/day on days 0, 1, and 2). Tissues were snap-frozen in liquid nitrogen and kept at −80°C until further analysis. During treatment, mice were allowed free access to water and food. Mice were sacrificed on day 3 using cervical dislocation and liver tissue and blood were collected.
8
BALB/c Mice in SPF Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male-specific pathogen-free (SPF) BALB/c mice (7–8 weeks of age, weighing 20–22 g) were purchased from GemPharmatech Co., Ltd. (Nanjing, China) (animal certificate number: SCXK 2018-0008) and housed in specific pathogen-free conditions (temperature: 21°C ± 2°C, humidity: 45% ± 10%, light: 12-h light–dark cycle). The protocol (permit number: JZLLSC20210065) was approved by the Animal Care and Use Committee of Jiangxi University of Traditional Chinese Medicine and conducted following the guidelines prescribed by the committee.
9
Balb/c Mice for Biomedical Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Six to eight weeks old female Balb/c mice, weighing 18–25 g, were purchased from the GemPharmatech Co., Ltd. All animals were housed in temperature, humidity, and light-controlled rooms, with water provided ad libitum. Animal welfare and experimental procedures were carried out in accordance with the Ethical Regulations on the Care and Use of Laboratory Animals of Anhui Medical University and were approved by the school committee for animal experiments (LLSC20200083).
10
Evaluating Tumor Growth and Metastasis in Athymic Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five-week-old male athymic BALB/c mice were purchased from GemPharmatech (Nanjing, China) and maintained under specific pathogen-free conditions. For the in vivo cell proliferation assay, Eca-109 cells were stably transfected and subcutaneously injected into either side of the posterior flanks of the mouse. The tumor volume was measured every few days (length × width2 × 0.5). At the end of the experiment, the mice were euthanized, and the tumors were removed, weighed, and then fixed for hematoxylin and eosin (H&E) and Ki-67 immunostaining analysis. To observe tumor metastasis in the lungs, treated Eca-109 cells were injected into the mice’s tail vein. After few days, the mice were euthanized, and the lungs were removed. Images of the lungs were taken for recording and used for H&E immunostaining analysis, and the nodules on the lung were counted. This study was conducted in strict accordance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The study protocol was approved by the Committee on the Ethics of Animal Experiments of Nanjing Medical University.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!