The largest database of trusted experimental protocols

Taok2 cdna

Manufactured by OriGene

TAOK2 cDNA is a laboratory product that provides the complementary DNA (cDNA) sequence for the TAOK2 gene. TAOK2 encodes a serine/threonine protein kinase that is involved in various cellular processes. This product can be used for research purposes, such as gene expression studies or the development of molecular assays.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using taok2 cdna

1

Cloning and Characterization of TAOK2 Domains

Check if the same lab product or an alternative is used in the 5 most similar protocols
Full-length human TAOK2 was PCR amplified from TAOK2 cDNA (Origene, isoform alpha), and inserted in vector sfGFP-C1 (Addgene #54579) using restriction sites HindIII and MfeI. Domain dissection mutants were subcloned from sfGFP-TAOK2 using restriction enzymes HindIII and MfeI (New England Biolabs). All resultant plasmids were verified by sequencing. GST-TAOK2-(1187–1235) was subcloned from the sfGFP-TAOK2 into the pGEX4T1 vector using sites SalI and NotI. BFP-STIM1 was created by subcloning BFP from BFP-Rab5 (#49147) and inserting into STIM-YFP (#19754) using restriction sites SalI and NotI.
+ Open protocol
+ Expand
2

Cloning and Characterization of TAOK2 Domains

Check if the same lab product or an alternative is used in the 5 most similar protocols
Full-length human TAOK2 was PCR amplified from TAOK2 cDNA (Origene, isoform alpha), and inserted in vector sfGFP-C1 (Addgene #54579) using restriction sites HindIII and MfeI. Domain dissection mutants were subcloned from sfGFP-TAOK2 using restriction enzymes HindIII and MfeI (New England Biolabs). All resultant plasmids were verified by sequencing. GST-TAOK2-(1187–1235) was subcloned from the sfGFP-TAOK2 into the pGEX4T1 vector using sites SalI and NotI. BFP-STIM1 was created by subcloning BFP from BFP-Rab5 (#49147) and inserting into STIM-YFP (#19754) using restriction sites SalI and NotI.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!