Immunoblot assay was performed, as previously described [25 (link)]. (1) anti-VEGF-A antibody (Abcam, Cambridge, UK), (2) anti-VEGFR2 antibody (Cell Signaling), (3) anti-type IV collagen antibody (Abcam), (4) monoclonal anti-human VASH2 antibody (clone 1760; provided by Tohoku University, Sendai, Japan), and (5) monoclonal anti-β-actin antibody (Sigma-Aldrich) were used as primary antibodies. Among these, anti-type IV collagen antibody was used under non-reducing condition. HRP-conjugated anti-rabbit or mouse IgG antibodies (Cell Signaling) were served as secondary antibodies. Images were obtained with ImageQuant LAS 4000 (GE Healthcare, Pittsburgh PA).
Anti type 4 collagen antibody
Manufactured by Abcam
Sourced in United Kingdom, United States
Anti-type IV collagen antibody is a laboratory reagent used to detect and quantify type IV collagen, a major structural component of basement membranes. This antibody can be used in various immunoassay techniques to investigate type IV collagen expression and distribution in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Imagequant las 4000, by GE Healthcare (1 mentions)
Anti β actin monoclonal antibody, by Merck Group (1 mentions)
Anti vegfr2 antibody, by Cell Signaling Technology (1 mentions)
Anti vegfa antibody, by Abcam (1 mentions)
Las 4000, by Cytiva (1 mentions)
Meyer s hematoxylin, by Merck Group (1 mentions)
Diamine benzidine hydrochloride, by Agilent Technologies (1 mentions)
2 protocols using anti type 4 collagen antibody
1
Immunoblot Analysis of VEGF Signaling
2
Immunohistochemical Evaluation of Collagen
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Consecutive 4-μm sections were immunohistochemically stained using the immunoperoxidase technique described previously[13 (link)]. Anti-type IV collagen antibody and anti-type III collagen antibody (Abcam, Cambridge, MA, United States) were used at a concentration of 0.2 μg/mL. Secondary antibodies (Medical and Biological Laboratories, Nagoya, Japan) were used at a concentration of 0.2 μg/mL. Tissue sections were color-developed with diamine benzidine hydrochloride (DAKO, Glastrup, Denmark), and counterstained with Meyer's hematoxylin (Sigma). We evaluated immunopositivity at the basement membrane and stromal fibers. Staining strength was scored from (-) to (++): (-) is no staining; (+) is staining equal to that of non-pathologic mucosa; (++) is staining more pronounced than that of non-pathologic mucosa. All samples were stained at one time to equalize staining conditions. For a negative control, unimmunized rat IgG (Santa-Cruz Biotechnology, Santa-Cruz, CA, United States) was used as primary antibody.
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