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Anti lrrc31 ab100379

Manufactured by Abcam
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Anti-LRRC31 (ab100379) is a recombinant antibody that recognizes the LRRC31 protein. LRRC31 is a leucine-rich repeat-containing protein. The antibody is suitable for use in various applications, such as Western blot and immunohistochemistry, to detect the LRRC31 protein.

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Lab products found in correlation

Nitrocellulose membrane, by Thermo Fisher Scientific (2 mentions) Loading buffer, by Thermo Fisher Scientific (2 mentions) Nupage bis tris gel, by Thermo Fisher Scientific (2 mentions) Odyssey clx system, by LI COR (2 mentions) Mper lysis buffer, by Merck Group (2 mentions) Irdye 800rd goat anti rabbit, by LI COR (2 mentions) Anti hsp90, by R&D Systems (2 mentions) Image studio, by LI COR (2 mentions) Irdye 680rd goat anti mouse, by LI COR (2 mentions) Sodium orthovanadate, by Merck Group (2 mentions) Qiazol, by Qiagen (2 mentions)

2 protocols using anti lrrc31 ab100379

1

Western Blot Analysis of Protein Lysates

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Total cell lysates were prepared from biopsy protein following Qiazol (QIAGEN) RNA extraction. Briefly, protein pellets were resuspended in Laemmli buffer, incubated at 37°C for 30 minutes, vortexed briefly, and heated to 95°C for 5 minutes before electrophoresis. Cells were lysed using MPER lysis buffer (Sigma-Aldrich, St. Louis, MO) supplemented with protease inhibitors (Roche) and sodium orthovanadate (10 mM) (Sigma-Aldrich). Loading buffer (Life Technologies) was added and samples were heated to 95°C for 5 minutes, subjected to electrophoresis on 4-12% NuPAGE Bis-Tris gels (Life Technologies), transferred to nitrocellulose membranes (Life Technologies), and visualized using the Odyssey CLx system (LI-COR Biosciences, Lincoln, NE) with IRDye 800RD goat anti-rabbit (LI-COR) and IRDye 680RD goat anti-mouse (LI-COR Biosciences) secondary antibodies. Primary antibodies were: anti-LRRC31 (ab100379; Abcam PLC, Cambridge, UK) and anti-HSP90 (AF7247; R&D Systems, Minneapolis, MN). Blots were quantified using Image Studio (LI-COR).
D’Mello R., Caldwell J., Azouz N., Wen T., Sherrill J., Hogan S, & Rothenberg M. (2015). LRRC31 is induced by IL-13 and regulates kallikrein expression and barrier function in the esophageal epithelium. Mucosal immunology, 9(3), 744-756.
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2

Western Blot Analysis of Protein Lysates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cell lysates were prepared from biopsy protein following Qiazol (QIAGEN) RNA extraction. Briefly, protein pellets were resuspended in Laemmli buffer, incubated at 37°C for 30 minutes, vortexed briefly, and heated to 95°C for 5 minutes before electrophoresis. Cells were lysed using MPER lysis buffer (Sigma-Aldrich, St. Louis, MO) supplemented with protease inhibitors (Roche) and sodium orthovanadate (10 mM) (Sigma-Aldrich). Loading buffer (Life Technologies) was added and samples were heated to 95°C for 5 minutes, subjected to electrophoresis on 4-12% NuPAGE Bis-Tris gels (Life Technologies), transferred to nitrocellulose membranes (Life Technologies), and visualized using the Odyssey CLx system (LI-COR Biosciences, Lincoln, NE) with IRDye 800RD goat anti-rabbit (LI-COR) and IRDye 680RD goat anti-mouse (LI-COR Biosciences) secondary antibodies. Primary antibodies were: anti-LRRC31 (ab100379; Abcam PLC, Cambridge, UK) and anti-HSP90 (AF7247; R&D Systems, Minneapolis, MN). Blots were quantified using Image Studio (LI-COR).
D’Mello R., Caldwell J., Azouz N., Wen T., Sherrill J., Hogan S, & Rothenberg M. (2015). LRRC31 is induced by IL-13 and regulates kallikrein expression and barrier function in the esophageal epithelium. Mucosal immunology, 9(3), 744-756.
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