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Quantikine human vegf

Manufactured by R&D Systems
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The Quantikine Human VEGF Immunoassay is a quantitative sandwich enzyme immunoassay designed to measure human vascular endothelial growth factor (VEGF) levels in cell culture supernates, serum, and plasma. It has a measuring range of 31.2 to 2,000 pg/mL.

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Lab products found in correlation

Synergy h1, by Agilent Technologies (1 mentions) Synergy 2 microplate reader, by Agilent Technologies (1 mentions) Originpro 8, by OriginLab (1 mentions) Thermomax, by Molecular Devices (1 mentions) Gene5, by Agilent Technologies (1 mentions) Synergy ht, by Agilent Technologies (1 mentions) Protein array reader, by Bio-Rad (1 mentions) Infinite 200 pro plate reader, by Tecan (1 mentions) Dve00, by R&D Systems (1 mentions) Quantikine human endoglin cd105, by R&D Systems (1 mentions) Dndg00, by R&D Systems (1 mentions) Glomax multi detection system, by Promega (1 mentions) Quantikine human tgf beta 1, by R&D Systems (1 mentions) Pdgf ab, by R&D Systems (1 mentions) Basic fibroblast growth factor (bfgf), by R&D Systems (1 mentions) Femto chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Supersignal west pico, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Spectramax 190, by Molecular Devices (1 mentions)

17 protocols using quantikine human vegf

1

VEGF Quantification in HRMEC Supernatant

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The VEGF levels of 200 µL of HRMECs culture supernatants diluted with PBS were measured using a Human VEGF Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems) according to the manufacturer's instructions. A 96-well microplate was coated with specific human VEGF monoclonal antibodies. The amount of cell VEGF was normalized to the total cell numbers.
Chen S., Zhang J., Sun D., Wu Y., Fang J., Wan X., Li S., Zhang S., Gu Q., Shao Q., Dong J., Xu X., Wei F, & Sun Q. (2023). SYVN1 Promotes STAT3 Protein Ubiquitination and Exerts Antiangiogenesis Effects in Retinopathy of Prematurity Development. Investigative Ophthalmology & Visual Science, 64(11), 8.
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2

Quantitative Analysis of Growth Factors in MSC-CM

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The concentration of major growth factors (VEGF, TGF-β1, and HGF) in MSC-CM was quantitatively analyzed by ELISA. The Human VEGF Quantikine (R&D SYSTEMS, SVE00; Minnesota, US), Human TGF-β1 Quantikine (R&D SYSTEMS, SB100B), Human HGF Quantikine Kits (R&D SYSTEMS, SHG00) were used for analyses according to the manufacturer’s instructions. The optical density of the samples was measured using a microplate reader (Biotek, Synergy H1; Vermont, US).
Kim S.N., Lee C.J., Nam J., Choi B., Chung E, & Song S.U. (2020). The Effects of Human Bone Marrow-Derived Mesenchymal Stem Cell Conditioned Media Produced with Fetal Bovine Serum or Human Platelet Lysate on Skin Rejuvenation Characteristics. International Journal of Stem Cells, 14(1), 94-102.
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3

VEGF and VEGF-C Quantification

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Cells were plated at 1.25 × 106 cells/mL. Conditioned media were collected 48–72 hours later and subjected to Human VEGF-Quantikine enzyme-linked immunosorbent assay (ELISA) or Human VEGF-C-Quantikine ELISA (R&D Systems), following manufacturer’s instructions using a Synergy2 microplate reader (BioTek).
Michaelsen S.R., Staberg M., Pedersen H., Jensen K.E., Majewski W., Broholm H., Nedergaard M.K., Meulengracht C., Urup T., Villingshøj M., Lukacova S., Skjøth-Rasmussen J., Brennum J., Kjær A., Lassen U., Stockhausen M.T., Poulsen H.S, & Hamerlik P. (2018). VEGF-C sustains VEGFR2 activation under bevacizumab therapy and promotes glioblastoma maintenance. Neuro-Oncology, 20(11), 1462-1474.
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4

Quantification of VEGF and IL-6

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Culture supernatants were collected and centrifuged at 1,000 × g for 5 min. Samples were snap-frozen and stored at –80°C until analysis. The Human VEGF Quantikine and Interleukin-6 (IL-6) Quantikine ELISA Kits (all from R&D Systems, Minneapolis, MN, United States) were employed according to the manufacturer’s instructions. Absorbance was measured in microplate reader Infinite® 200 PRO (NanoQuant, Tecan Trading AG, Männedorf, Switzerland).
Franchi-Mendes T., Lopes N, & Brito C. (2021). Heterotypic Tumor Spheroids in Agitation-Based Cultures: A Scaffold-Free Cell Model That Sustains Long-Term Survival of Endothelial Cells. Frontiers in Bioengineering and Biotechnology, 9, 649949.
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5

Quantification of Axitinib and VEGF Levels

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Plasma samples for analysis of axitinib and vascular endothelial growth factor ligand (VEGF) concentrations were collected on the same days as PET/CT scans: −3 to 0 days prior to dosing, after 12–14 days of dosing (week 2) and after 5–7 days of drug washout (week 3). VEGF concentrations were measured by a commercially available 96-well plate quantitative sandwich immunoassay (Quantikine® human VEGF, R&D Systems, Minneapolis, MN) according to manufacturer’s instructions. Axitinib plasma concentrations were measured by a validated LC/MS/MS as previously described [11 (link)].
Scarpelli M., Bruce J.Y., Carmichael L., Eickhoff J., Kolesar J., Perlman S., Jeraj R, & Liu G. (2016). 18F-FLT PET/CT imaging in patients with advanced solid malignancies treated with axitinib on an intermittent dosing regimen. Cancer chemotherapy and pharmacology, 78(6), 1245-1252.
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6

VEGF Quantification by ELISA

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Free VEGF levels were measured by a commercially available ELISA kit (Quantikine® Human VEGF, R&D Systems®, Minneapolis, MN, USA), which detects VEGF-A in human serum, with a detection limit of 9 ng/L, a linearity range of 82%–107%, and an intra-assay CV% 6.7% [45 ]. As per the manufacturer’s instructions, the standard curve was generated with VEGF concentrations ranging from 31.2 to 2000 ng/L. All standards and samples were prepared in duplicates and the optical density was measured at 450 nm, with a correction at 550 nm, using an ELISA plate reader (ThermoMax, Molecular Devices). Data were linearized by plotting the log of the concentrations versus the log of the optical density, and the best-fit line of the standard curve was determined by regression analysis (OriginPro 8.0 software, OriginLab® Corporation, Northampton, MA, USA) [45 ].
Papachristos A., Karatza E., Kalofonos H, & Sivolapenko G. (2020). Pharmacogenetics in Model-Based Optimization of Bevacizumab Therapy for Metastatic Colorectal Cancer. International Journal of Molecular Sciences, 21(11), 3753.
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7

Quantitative Analysis of VEGF Secretion

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The secreted VEGF in DU-145 cell line was determined quantitatively using a commercial ELISA kit (cat. no. DVE00, Quantikine Human VEGF; R&D Systems, Inc.) according to the manufacturer's instructions. In brief, cell supernatant was diluted at 1:3 with assay diluent, 150 µl supernatant was added to wells, incubated at room temperature for 2 h and washed three times with 300 µl wash buffer. Then, 200 µl Human VEGF Conjugate was added to each well and incubated and washed as aforementioned. Finally, 200 µl substrate solution was added, color was developed for 20 min and the reaction was stopped with 50 µl STOP solution. Absorbance was measured at 450 nm using a microplate reader (Synergy HT; BioTek Instruments, Inc.); data are expressed as pg/ml (analyzed using Gene5, version 3.08, biotek.com/products/software-robotics–software/gen5-microplate-reader–and-imager-software/).
Méndez-Clemente A., Bravo-Cuellar A., González-Ochoa S., Santiago-Mercado M., Palafox-Mariscal L., Jave-Suárez L., Solorzano-Ibarra F., Villaseñor-García M., Ortiz-Lazareno P, & Hernández-Flores G. (2022). Dual STAT-3 and IL-6R inhibition with stattic and tocilizumab decreases migration, invasion and proliferation of prostate cancer cells by targeting the IL-6/IL-6R/STAT-3 axis. Oncology Reports, 48(2), 138.
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8

Evaluating Skeletal Myoblast Competence for Cardiac Transplantation

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Example 2

Subsequently, the two kinds of sheet-shaped cell cultures of skeletal myoblasts, Sheet A and Sheet B, were examined for the capacity of yielding cytokine by using the following measurement kits. Quantikine Human VEGF (made by R&D Systems Inc.), Quantikine Human CXCL 12/SDF-1α (made by R&D Systems Inc.), and RayBio Human HGF ELISA (RayBiotech, Inc.). Measurement was carried out according to the protocol specified by each kit.

The results are shown in FIGS. 1 and 2. The rate of multinucleation is around 16.8% for myo A and around 29.6% for myo B (see FIG. 1). As regards the cytokine yielding capacity, Sheet B is much higher than Sheet A for HGF, VEGF, and SDF-1 (see FIG. 2). These species of cytokine are said to be concerned with the regeneration of cardiac muscle. The foregoing results suggest that Sheet B, which is composed of skeletal myoblasts myo B having a high rate of multinucleation, is more competent for transplantation to the heart than Sheet A.

As above-described, the use of the method according to the present invention has for the first time made it possible to evaluate the competence of grafts for transplantation. The result of evaluation helps improve the effect of treatment by transplantation.

US09909161B2. Method and system for evaluation of grafts (2018-03-06). Terumo Kabushiki Kaisha [JP]. Inventors: Kenta Sakamoto [JP].
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9

Quantitative VEGF Immunoassay Protocol

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Serum VEGF concentration was measured by the sandwich enzyme-linked immunosorbent assay kit for human VEGF (Quantikine human VEGF; R&D System, Minneapolis, MN, USA). The assay was performed according to the manufacturer’s instructions. The reaction mixture was quantified using the Bio-Plex protein array reader. The VEGF levels were automatically calculated by Bio-Plex Manager software using the appropriate standard curve. The detection limit of VEGF was 31.2 pg/mL.
Chen C.Y., Huang S.H., Chien K.J., Lai T.J., Chang W.H., Hsieh K.S, & Weng K.P. (2022). Reappraisal of VEGF in the Pathogenesis of Kawasaki Disease. Children, 9(9), 1343.
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10

Quantification of VEGF in Conditioned Media

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VEGF levels in CM collected as described above were measured by a commercially available ELISA kit (Quantikine® Human VEGF, R&D Systems®, Minneapolis, MN, USA). As per the manufacturer’s instructions, the standard curve was generated with VEGF concentrations ranging from 31.2 to 2000 ng/L. All standards and samples were prepared in duplicates and the optical density was measured at 450 nm, with a correction at 550 nm, using an Infinite 200 Pro plate reader (Tecan, Männedorf, Switzerland).
Toboni M.D., Lomonosova E., Bruce S.F., Tankou J.I., Mullen M.M., Schab A., Oplt A., Noia H., Wilke D., Kuroki L.M., Hagemann A.R., McCourt C.K., Thaker P.H., Powell M.A., Khabele D., Mutch D.G, & Fuh K.C. (2021). Inhibition of AXL and VEGF-A Has Improved Therapeutic Efficacy in Uterine Serous Cancer. Cancers, 13(23), 5877.
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