Dy1160

Manufactured by R&D Systems

Sourced in United Kingdom

The DY1160 is a compact and versatile lab equipment designed for high-precision fluid handling. It features a variable-speed peristaltic pump that can be used for a wide range of applications, including dosing, filling, and sample transfer. The device is constructed with durable materials and offers precise flow rate control.

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Lab products found in correlation

4 protocols using dy1160

Quantifying Secreted Osteocalcin and Inflammatory Markers

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Human osteocalcin DuoSet ELISA (R&D Systems; DY1419) was used to measure total secreted osteocalcin in cell culture media. Endothelin Pan Specific, ICAM‐1/CD54, VCAM‐1/CD106, and total MMP‐3 DuoSet ELISAs were performed on cell culture media according to the manufacturer's instructions (Catalog numbers DY1160, DY720, DY809, and DY513; R&D Systems, Abingdon, UK).

Millar S.A., Anderson S.I, & O'sullivan S.E. (2019). Human vascular cell responses to the circulating bone hormone osteocalcin. Journal of Cellular Physiology, 234(11), 21039-21048.

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Quantifying Secreted Inflammatory Factors

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Secreted interleukin‐6 (IL‐6), vascular cell adhesion molecule‐1 (VCAM‐1), endothelin (ET), and monocyte chemoattractant protein‐1 (MCP‐1) were measured in cell culture media by ELISA as per manufacturers' instructions (catalogue numbers DY206, DT809, DY1160, and DY279; R&D Systems). A lactate dehydrogenase (LDH) (Colorimetric) Assay Kit (category number ab102526; Abcam) was performed on cell media as per the manufacturer's instructions. A bicinchoninic acid protein assay was performed to quantify the total protein content in the cell lysates collected at the end of the experiments (P. K. Smith et al., 1985). A total osteocalcin ELISA which does not differentiate between uncarboxylated and carboxylated osteocalcin was performed to assess predicted and actual concentrations of ucOC added to experimental wells and to validate the purchased protein (DY1419; R&D Systems).

Tacey A., Millar S., Qaradakhi T., Smith C., Hayes A., Anderson S., Zulli A., O'Sullivan S, & Levinger I. (2020). Undercarboxylated osteocalcin has no adverse effect on endothelial function in rabbit aorta or human vascular cells. Journal of Cellular Physiology, 236(4), 2840-2849.

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Multiplex Cytokine Profiling of Cell Culture

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Total OCN, endothelin pan specific, intracellular adhesion molecule‐1 (ICAM‐1)/CD54, vascular cell adhesion molecule‐1 (VCAM‐1)/CD106, total MMP‐3, CCL2/monocyte chemoattractant protein‐1 (MCP‐1), IL‐8/CXCL8, and IL‐17 DuoSet enzyme‐linked immunosorbent assays (ELISA) were performed on cell culture media as per the manufacturer's instructions (catalog no. DY1419; R&D systems, DY1160, DY720, DY809, DY513, DY279, DY208, and DY317). IL‐6 ELISA Ready‐SET‐Go! was performed on cell culture media as per the manufacturer's instructions (catalog no. 88‐7066‐22; Affymetri; eBioscience).

Millar S.A., Zala I., Anderson S.I, & O'Sullivan S.E. (2019). Osteocalcin does not influence acute or chronic inflammation in human vascular cells. Journal of Cellular Physiology, 235(4), 3414-3424.

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Quantification of Secreted Factors in aVICs and qVICs

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Male and female aVICs and qVICs were seeded in 10 cm dishes at a density of 50,000 cells/cm² and cultured in standard growth medium (low-glucose DMEM with 10% FBS and 1% penicillin-streptomycin solution) for 48 h. The aVICs were cultured on 10 cm tissue culture coated plates while qVICs were cultured on 10 cm collagen-coated plates in order to retain their qVIC phenotype for the duration of the experiment. Seven ELISAs were run on the conditioned culture media and culture lysate (collected in RIPA buffer, 89900; ThermoFisher): endothelin-1 (DY1160; R&D Systems, Minneapolis, MN), platelet derived growth factor-A (PDGF-A, DY221; R&D), vascular endothelial growth factor-A (VEGF-A, DY293B; R&D), basic fibroblast growth factor (bFGF, DY233; R&D), epidermal growth factor (EGF, DY236; R&D), insulin-derived growth factor-1 (IGF-1, DY291; R&D), and thrombospondin-2 (TSP2, DTSB20; R&D), all per manufacturer's instructions. The media and lysate were differentially diluted in the sample dilution buffer specific to each ELISA kit such that all sample absorbance readings fell within the dynamic range of each ELISA. A Quant-iT™ PicoGreen™ dsDNA Assay (P11496; Thermo Fisher Scientific) was performed on the lysate to normalize to cell number across all samples.

Nelson V., Patil V., Simon L.R., Schmidt K., McCoy C.M, & Masters K.S. (2021). Angiogenic Secretion Profile of Valvular Interstitial Cells Varies With Cellular Sex and Phenotype. Frontiers in Cardiovascular Medicine, 8, 736303.

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