Streptomycin mixture

The mouse insulinoma cell line MIN6 was obtained from BoGu Biotechnology Co. Ltd. (Shanghai, China). High-glucose (4500 mg/L) Dulbecco's modified Eagle's medium (DMEM) was purchased from Hyclone (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from Biological Industries (Cromwell, CT, USA). The MIN6 cells were maintained in high-glucose DMEM supplemented with 12% FBS, 10 μl/L β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA), 100 U/ml penicillin, and 100 μg streptomycin mixture (Solarbio, Beijing, China) at 37°C in 5% CO₂.

Human melanoma A375 cells (Cat no. SCSP-533) and mouse melanoma B16 cells (Cat no. TCM 2) were obtained from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences China (Shanghai, China). The cells were cultured in a sterile cell culture chamber (HF240, HEALFORCE, Shanghai Lishen Scientific Equipment Co. Ltd.) with 95% air and 5% CO₂ saturated humidity at a temperature of 37 °C. Human melanoma A375 cells were subsequently cultured and tested using DMEM high glucose medium (Cat no. SH30022.01, Hyclone) supplemented with 10% FBS (Cat no. 10091-148, Gibco), 1% streptomycin mixture (Cat no. P1400, Solarbio), and 1% sodium pyruvate (Cat no. SP0100, Solarbio). Mouse B16 cells were cultured in 1640 medium (Cat no. SH30809.01, Hyclone) supplemented with 10% FBS and 1% streptomycin mixture.

The mouse insulinoma cell line MIN6 was obtained from BoGu Biotechnology Co. Ltd. (Shanghai, China). High-glucose (4500 mg/L) Dulbecco's Modified Eagle's Medium (DMEM) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fetal bovine serum (FBS) was purchased from Biological Industries (Cromwell, CT, USA). The MIN6 cells were maintained in high-glucose DMEM supplemented with 12% FBS, 10 μl/L β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA), 100 U/ml penicillin, and 100 μg streptomycin mixture (Solarbio, Beijing, China) at 37°C in 5% CO₂.