Quant tag biotin kit

Manufactured by Vector Laboratories

Sourced in United States

The Quant*Tag Biotin Kit is a labeling reagent used for the detection and quantification of biotinylated biomolecules, such as proteins, nucleic acids, and carbohydrates. The kit provides a simple and reliable method for the detection of biotin-labeled targets.

Automatically generated - may contain errors

Lab products found in correlation

Zeba desalt spin column, by Thermo Fisher Scientific (2 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions) Advanced protein assay, by Cytoskeleton (1 mentions) Ez link sulfo nhs lc biotin, by Thermo Fisher Scientific (1 mentions) Nhs peg12 biotin, by Thermo Fisher Scientific (1 mentions) Phadia 100, by Thermo Fisher Scientific (1 mentions)

6 protocols using quant tag biotin kit

Biotinylation of Allergen Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

EZ-Link Sulfo-NHS-LC-Biotin (Thermo Scientific, Rockford, IL) was added to a defined amount of each allergen (2 mg for chitinase, 0.25 mg for Bla g 3) at a 10-fold molar excess and incubated for 30 min at room temperature. The biotinylated mix was put over a pre-washed Zeba Desalt Spin Column (Thermo Scientific, Rockford, IL) two times and the concentration was determined after biotinylation by Advanced Protein Assay (Cytoskeleton, Denver, Colorado).
The quantification of biotinylation was carried out by using a Quant Tag™ Biotin Kit (Vector Laboratories, Burlingame, CA). Samples were tested in triplicate against a known biotin standard curve to determine the number of biotins per allergen molecule.

Pomés A., Schulten V., Glesner J., da Silva Antunes R., Sutherland A., Bacharier L.B., Beigelman A., Busse P., Frazier A, & Sette A. (2021). IgE and T Cell Reactivity to a Comprehensive Panel of Cockroach Allergens in Relation to Disease. Frontiers in Immunology, 11, 621700.

+ Open protocol

+ Expand

In Vivo Trafficking and Excretion of Biotinylated Graphene Quantum Dots

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For in vivo trafficking and analysis of excretion levels, biotinylated GQDs were intraperitoneally infused. To determine the remaining amounts of GQDs, mesentery tissue was collected after mice were sacrificed. After the tissue was minced and homogenized, it was incubated with FITC-conjugated anti-biotin antibody (BD Biosciences). Fluorescence was assessed using flow cytometric analysis. In addition, urine samples were also collected at designated time points to investigate excretion as described in a previous report (14 (link)). The concentrations of the GQDs in urine samples were measured by the Quant*Tag Biotin Kit (Vector Laboratories Inc., Burlingame, CA, USA).

Lee B.C., Lee J.Y., Kim J., Yoo J.M., Kang I., Kim J.J., Shin N., Kim D.J., Choi S.W., Kim D., Hong B.H, & Kang K.S. (2020). Graphene quantum dots as anti-inflammatory therapy for colitis. Science Advances, 6(18), eaaz2630.

+ Open protocol

+ Expand

Labeling Luciferase Plasmid with Streptavidin-Conjugated Qdots

Check if the same lab product or an alternative is used in the 5 most similar protocols

Luciferase plasmid was labeled with Qdot™ 585 Streptavidin Conjugate (QD585, ThermoFisher) utilizing the DNA-intercalating crosslinker Psoralen-PEO-biotin (ThermoFisher) as previously described with minor protocol modifications.[38 (link)] In brief, the psoralen crosslinker (20 mM in DMSO) was diluted 1:100 v/v with luciferase plasmid DNA (2 mg/mL in water) and irradiated using a 50 W longwave UV lamp for 1 h at room temperature. The plasmid was ethanol/acetate precipitated and biotin content verified to be in excess of 1000 biotins/plasmid using the Quant*Tag™ Biotin Kit (Vector Laboratories) and a Nanodrop spectrophotometer (ThermoFisher). Strep-QD585 solution was mixed with biotinylated plasmid at 1:1 molar ratio and incubated at room temperature overnight. Plasmids were ethanol/acetate precipitated to remove unconjugated Strep-QD585 and resuspended in 5% glucose for use in transfection. Conjugation purity was verified by gel electrophoresis and the degree of conjugation was determined to be between 0.5–1 QD585/pLuciferase using the HS dsDNA Qubit assay (ThermoFisher) to quantify DNA concentration and a fluorescence plate reader to quantify QD585 concentration via a custom standard curve.

Peeler D.J., Luera N., Horner P.J., Pun S.H, & Sellers D.L. (2020). Polyplex transfection from intracerebroventricular delivery is not significantly affected by traumatic brain injury. Journal of controlled release : official journal of the Controlled Release Society, 322, 149-156.

+ Open protocol

+ Expand

Production of His-tagged or Biotinylated Salipro-PANX1

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

To generate His-tagged or biotinylated Salipro-PANX1 particles, the same method as described above was used to extract and purify Salipro-PANX1 using His-tagged or biotinylated saposin A. His-tagged saposin A was produced and purified as previously described^{3 (link),6}, albeit without the TEV protease cleavage step for His-tag removal. Saposin A was biotinylated in a one-hour incubation step at RT using NHS-PEG12-Biotin (Thermo Fisher Scientific), following the manufacturer’s recommendations. The Quant*Tag™ Biotin Kit (Vector Laboratories) was used to confirm the amount of saposin A biotinylation.

Drulyte I., Gutgsell A.R., Lloris-Garcerá P., Liss M., Geschwindner S., Radjainia M., Frauenfeld J, & Löving R. (2023). Direct cell extraction of membrane proteins for structure–function analysis. Scientific Reports, 13, 1420.

+ Open protocol

+ Expand

IgE Antibody Binding Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Direct IgE antibody binding assays were performed by ImmunoCAP. Biotinylation of
the allergens was carried out using the EZ-Link Sulfo-NHS-LC-Biotin, No-Weigh™
Format (Thermo Fisher Scientific Inc., Pierce, Rockford, IL), with biotin added at a
10-fold molar excess to the allergens. Zeba™ Desalt Spin Columns (Thermo Fisher
Scientific Inc., Pierce, Rockford, IL) were used to remove any excess biotin from the
biotinylated allergens. A Quant*Tag Biotin Kit™ (Vector Laboratories, Inc.,
Burlingame, CA) was used to quantify the number of biotins per molecule. IgE detection and
quantification in plasma samples was determined using Specific IgE detection
streptavidin-ImmunoCAP on the Phadia® 100 (Thermo Fisher Scientific, Portage, MI).
After testing several concentrations of allergen (from 0.25 to 10 µg/ml),
streptavidin ImmunoCAPs were loaded with 5 µg/CAP of biotinylated allergen and
incubated for 30 minutes. IgE measurements were performed according to standard ImmunoCAP
procedures.

Glesner J., Vailes L.D., Schlachter C., Mank N., Minor W., Osinski T., Chruszcz M., Chapman M.D, & Pomés A. (2016). Antigenic determinants of Der p 1: Specificity and cross-reactivity associated with IgE antibody recognition. Journal of immunology (Baltimore, Md. : 1950), 198(3), 1334-1344.

+ Open protocol

+ Expand

Synthesis and Purification of Biotin-Draxin22

Check if the same lab product or an alternative is used in the 5 most similar protocols

NH2-PEG2-Suc-Gly-Glu(OtBu)-Val-Met-Pro-

Thr(tBu)-Leu-Asp(OtBu)-Met-Ala-Leu-Phe-Asp(OtBu)-

Trp(Boc)-Thr(tBu)-Asp(OtBu)-Tyr(tBu)-Glu(OtBu)-Asp(OtBu)-Leu-Lys(Boc)-Pro was assembled from RINK-ChemMatrix resin using the standard Fmoc protocol. A mixture of Biotin-OSu and DIPEA in DMF was added to the protected peptide resin to produce a biotin-modified derivative. The resin was washed with methanol and dried in vacuo. An aliquot of the resin was treated with a mixture of TFA/water/ethanedithiol/triisopropylsilane (94:2.5:2.5:1) for 2 hr, followed by precipitation in cold dimethyl ether. The crude peptide was purified by HPLC on a Cosmosil 5C18 ARII column (4.6 × 250 mm) with two solvent systems and the gradient elution method at a flow rate of 1 mL/min. Solvent A was 0.1% TFA in water, and solvent B was 0.1% TFA in acetonitrile. After lyophilization, Biotin-Draxin22 was obtained as a white powder. Draxin 22aa was measured by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy. The calculated mass for (M+H)+ was 3041.40. The concentration analysis of Draxin 22aa was quantified using Quant*Tag Biotin kit (VECTOR Laboratories, CA, USA).

Sato Y., Matsuo A., Kudoh S., Fang L., Hasegawa K., Shinmyo Y, & Ito T. (2018). Expression of Draxin in Lung Carcinomas. Acta Histochemica et Cytochemica, 51(1), 53-62.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!