PC12 cells derived from rat pheochromocytoma were purchased from ATCC and cultured in RMPI 1640 culture medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum at 37°C in a 5% CO2 incubator. Culture medium was refreshed once every 3 days. Aβ42 (Abcam, cat # ab120301, USA) was dissolved in dimethyl sulfoxide (DMSO) to a final concentration of 1 mM and then incubated at 37°C for 4 days. Maackiain (Sigma-Aldrich) was dissolved into dimethyl sulfoxide to a concentration of 10 mM. Fresh PC12 cell culture medium was added 6 h prior to Aβ42 stimulation.
Maackiain
Manufactured by Merck Group
Sourced in United States
Maackiain is a laboratory product developed by Merck Group. It is a chemical compound used for research purposes. Maackiain is often utilized in various scientific experiments and analyses, but a detailed description of its core function cannot be provided without extrapolation or interpretation.
Automatically generated - may contain errors
2 protocols using maackiain
1
PC12 Cell Culture and Amyloid-Beta Stimulation
2
Phytoalexin Quantification by HPLC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Phytoalexins were identified and quantified by HPLC using an HPLC (Perkin Elmer, Series 200 System, Monza, Italy) equipped with a diode array detector set to 210 and 220 nm.
Analyses were carried out in duplicate injecting 20 μL of all samples for each analysis.
Standards (scopoletin, pisatin, medicarpin, maackiain (Sigma-Aldrich, St. Luis, MO, USA)) were prepared at concentration of 1 mg ml -1 in methanol and diluted in a 1:10 ratio in a C 18 column 250×4 mm internal diam., containing 5 mm diam. beads (LiChrospher 100 RP-18), using a flow rate of 1 ml min -1 and elution with CH 3 CN/H 2 O along a linear gradient of CH 3 CN from 20 to 33% over a period of 90 min, and again from 33 to 20% for 5 min. The column was re-equilibrated under isocratic conditions for 5 min before the next run. The data obtained were expressed in µg g -1 FW.
Analyses were carried out in duplicate injecting 20 μL of all samples for each analysis.
Standards (scopoletin, pisatin, medicarpin, maackiain (Sigma-Aldrich, St. Luis, MO, USA)) were prepared at concentration of 1 mg ml -1 in methanol and diluted in a 1:10 ratio in a C 18 column 250×4 mm internal diam., containing 5 mm diam. beads (LiChrospher 100 RP-18), using a flow rate of 1 ml min -1 and elution with CH 3 CN/H 2 O along a linear gradient of CH 3 CN from 20 to 33% over a period of 90 min, and again from 33 to 20% for 5 min. The column was re-equilibrated under isocratic conditions for 5 min before the next run. The data obtained were expressed in µg g -1 FW.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!