Rotary vacuum evaporator n n series

The vacuum-dried P. avium samples (5 g) were extracted with 43% acidified ethanol (0.1% HCl) with a solid-to-liquid ratio of 1 g/15 mL using a sonicator as described by Milić, Daničić (37 (link)) with slight modifications. The volumetric flasks containing solutions were placed in an ultrasonic bath (Elmasonic E30H, GmbH, Germany) at a frequency of 30 kHz for 42 min at 54°C. Afterwards, the mixtures were centrifuged at 9000 rpm, 4°C for 10 min (MPW-352R, refrigerated laboratory centrifuge, United States). After the filtration of the extract with a vacuum filter, subsequent ethanol evaporation was carried out using a rotary evaporator under controlled conditions, i.e., 40°C temperature and 0.1 MPa pressure (EYELA Rotary Vacuum Evaporator N-N Series). The obtained extract was then stored at -20°C till further usage.

This study used dried rosebuds from 24 newly crossbred Korean roses (Rosa hybrida) from Gumi Floriculture Research Institute (Gumi, Republic of Korea) in 2021, which are (1) Lover Shy, (2) Lovely Scarlet, (3) Loving Heart, (4) Red Perfume, (5) Luminus, (6) Mirinae Gold, (7) Betty, (8) Bichina, (9) Aileen, (10) Onnuri, (11) Yunina, (12) Jaemina Red, (13) Jinseonmi, (14) Chilbaegri, (15) Tamina, (16) Tamnari, (17) Pretty Velvet, (18) Peach Grace, (19) Pink Love, (20) Pink Perfume, (21) Hanaro, (22) Hanaram, (23) Hanggina, and (24) Ice Wing. Since total rosebud extracts were higher in antioxidative activity than petal extracts, in addition to having higher yield, we used total rosebuds [38 (link)].
Based on our previous study, the rosebuds were extracted with 80% ethanol to achieve high polyphenol content [39 (link)]). The dried rosebuds were pulverized in a rotor mill (Laval Lab Inc., Laval, QC, Canada), immersed in 80% ethanol in an ultrasonic water bath, heated at 60~70 °C for 2 h, and then ultrasonic-extracted for 1 h. Actually, the extraction solvent/solid ratio was set to 49:1 (980 mL 80% ethanol/20 g dried rosebuds). After extraction, the mixture was cooled, filtered, and then concentrated under a reduced pressure to 50 brix using a vacuum evaporator (Rotary Vacuum Evaporator N-N series; Eyela, Tokyo, Japan), and then used as a test sample.

The dried Aloe arborescens (100 g) was extracted in 900 ml of distilled water at 100°C for 24 hours using the extractor installed with a vertical reflux condenser (GLHMR B1000, Global Lab, Siheung, Korea). The extracted samples were concentrated by rotary vacuum evaporator (Rotary Vacuum Evaporator N-N series, EYELA, Rikakikai Co., Tokyo, Japan) and lyophilized to freeze dryer (RV8, Edwards, Sweden). To obtain the fermented aloe extract, 100 g of dried Aloe arboresecens was extracted with 900 mL of distilled water at 100°C for 24 hours with a reflux condensed extractor (GLHMR B1000, Global Lab, Siheung, Korea). Then, the whole extracts were mixed with the following basal medium: 0.5% yeast extract, 1% peptone, 2% glucose, 0.01% magnesium sulfate, 0.005% manganese sulfate, 0.2% potassium phosphate, 0.1% polysorbate 80. After that, 3% (v/v) of Lactobacillus brevis were inoculated into a medium which was already autoclaved at 121°C for 30 minutes. The incubator was operated at 100 rpm and 37°C for 3 days. Then, the culture broth was lyophilized to make a powder by a freeze dryer (RV8, Edwards, Sweden). The powders were stored at −20°C before use.