SOD and GPx activities were measured in the supernatant by using ZellBio kits (Zellbio GmbH, Ulm, Germany). After adding reagents, samples, and standards into the wells, absorbance was measured at 0 and 2 mins with an ELISA microplate reader (AD Touch Reader, apDia, Belgium) at 412 nm. The concentrations of SOD and GPx were expressed as U/mg protein. The sensitivity of the assay for SOD and GPx was 1 U/mL and 5 U/mL, respectively.
Zell bio kits
Manufactured by ZellBio
Sourced in Germany
The Zell Bio kits are a collection of laboratory equipment designed for various scientific applications. Each kit contains a set of tools and reagents necessary for specific experimental procedures. The core function of these kits is to provide researchers with a standardized and convenient way to perform a range of laboratory tasks.
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Lab products found in correlation
6 protocols using zell bio kits
1
Superoxide Dismutase and Glutathione Peroxidase Assay
2
Assessing Lipid Peroxidation in Lung
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Malondialdehyde (MDA) is the end product of the major chain reactions leading to oxidation of fatty acids, and measurement of MDA content is the most widely used method for assessing lipid peroxidation. The amount of lipid peroxidation was assessed by thiobarbituric acid reactive substances (TBARS) in the lung. MDA activity was determined colorimetrically at 532 nm. All parameters were evaluated using diagnostic Zell Bio kits and according to the manufacturer’s instructions (Zell Bio, Germany).
3
Measurement of SOD and GPx Activities
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SOD and GPx activities were measured in the supernatant by using ZellBio kits (Zellbio GmbH, Ulm, Germany). After adding reagents, samples, and standards into the wells, absorbance was measured at 0 and 2 mins with an ELISA microplate reader (AD Touch Reader, apDia, Belgium) at 412 nm. The concentration of SOD and GPx was expressed as IU/mg protein. The sensitivity of the assay for SOD and GPx was 1U/mL and 5U/mL, respectively.
4
Ovary Tissue Homogenization and MDA Measurement
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Ovary homogenates were prepared by mixing 0.5 g of ovary tissue with 5 ml of phosphate buffer saline and homogenized by homogenizer (Polytron, Heidolph RZR 1, Germany). The suspension was centrifuged at 12,000 g for 15 min at 4 C (Biofuge Primo R, Heraeus, Germany). The supernatant was taken for measuring MDA. The samples were analyzed directly. This parameter has been measured using diagnostic Zell Bio kits and according to the manufactures recipes (Zell Bio, Germany).
5
Plasma Biomarker Analysis in Avian Study
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On the morning of day 38, blood samples (5 ml) were collected from the same three
birds in each pen by venipuncture (wing vein). Heparin-containing glass tubes
were used to collect the samples. Blood samples were centrifuged at
3,500×g for 10 min at 4°C to obtain plasma. The obtained
heparinized plasma samples were stored at -20°C until further analyses of
glucose, albumin, total protein, uric acid, blood urea nitrogen (BUN),
creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST),
lactate dehydrogenase (LDH), and creatine kinase (CK) by commercial enzymatic
kits (Pars Azmoon Kits, Pars Azmoon Inc., Tehran, Iran) using a plasma
autoanalyzer (Abbott Laboratories, Illinois, US). Plasma samples were also
employed to determine total antioxidant capacity (TAC), malondialdehyde (MDA),
and NO levels. Plasma TAC and MDA levels were measured using Zellbio kits
(ZellBio GmbH, Ulm, Germany) by the colorimetric method as described by the
manufacturer. Moreover, plasma NO was measured by the total NO assay kit
(NatrixTM, West Azerbaijan, Iran) according to the manufacturer’s
instructions.
birds in each pen by venipuncture (wing vein). Heparin-containing glass tubes
were used to collect the samples. Blood samples were centrifuged at
3,500×g for 10 min at 4°C to obtain plasma. The obtained
heparinized plasma samples were stored at -20°C until further analyses of
glucose, albumin, total protein, uric acid, blood urea nitrogen (BUN),
creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST),
lactate dehydrogenase (LDH), and creatine kinase (CK) by commercial enzymatic
kits (Pars Azmoon Kits, Pars Azmoon Inc., Tehran, Iran) using a plasma
autoanalyzer (Abbott Laboratories, Illinois, US). Plasma samples were also
employed to determine total antioxidant capacity (TAC), malondialdehyde (MDA),
and NO levels. Plasma TAC and MDA levels were measured using Zellbio kits
(ZellBio GmbH, Ulm, Germany) by the colorimetric method as described by the
manufacturer. Moreover, plasma NO was measured by the total NO assay kit
(NatrixTM, West Azerbaijan, Iran) according to the manufacturer’s
instructions.
6
Antioxidant Enzyme Levels in Diabetic Patients
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Blood samples (5 mL) were taken from diabetic patients before and after the end of the three-month treatment period (after 12 h of overnight fasting and before taking the medication). Blood samples were poured into anticoagulant tubes and serum was separated by centrifugation (3,000 g for 15 min). Serum samples were stored in the freezer at a temperature of −70°C to be used to assess the serum levels of antioxidant enzymes after the end of the intervention period and serum collection from all patients. Serum levels of antioxidant enzymes (SOD, CAT, and GPx) were measured using Zell Bio kits (GmbH, Germany) and the method of enzyme-linked immunosorbent assay (ELISA).
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