Autoflex 2 maldi tof lrf50 cid

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied to determine the ranges of molecular weights of the keratin samples. Before the measurement, keratin samples were dissolved in a mixture of 1.5% dithiothreitol (DTT), 0.5 M tris hydrochloride (HCl), 10% glycerol and 2% sodium dodecyl sulfate (SDS). Sinapinic acid was selected as the matrix and dissolved to saturation in a mixture of 0.1–0.3% trifluoroacetic acid (TFA) and 50% acetonitrile. One microliter of the matrix mixture and sample were placed on the target plate and dried under air. The analysis was conducted using a Bruker mass spectrometer Autoflex II Maldi-TOF LRF50-CID (Bruker Daltonik GmbH, Germany).

Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) was applied to determine the molecular weight distribution of the keratin samples. Prior to the measurements, the keratin samples were dissolved in a mixture of 1.5% dithiothreitol (DTT), 0.5 M tris hydrochloride (HCl), 10% glycerol, and 2% sodium dodecyl sulphate (SDS). Sinapinic acid was selected as the matrix and dissolved to saturation in a mixture of 0.1–0.3% trifluoroacetic acid (TFA) and 50% acetonitrile. One microliter of the matrix mixture and sample was placed on the target plate and dried under air. The analysis was conducted using a Bruker mass spectrometer Autoflex II Maldi-TOF LRF50-CID (Bruker Daltonik GmbH, Germany).