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Ctx 1

Manufactured by Immunodiagnostic Systems
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Sourced in United Kingdom, United States, China

CTX-1 is a laboratory instrument designed for the detection and quantification of biological analytes. It utilizes an immunoassay-based detection method to provide accurate and reliable results. The core function of the CTX-1 is to facilitate the analysis of samples for various applications, such as clinical diagnostics, research, and drug development.

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Lab products found in correlation

Osteocalcin, by Biomedical Technologies (1 mentions) Trap 5b, by Immunodiagnostic Systems (1 mentions) Phosphate colorimetric assay kit, by Abcam (1 mentions) Ctx 2, by Immunodiagnostic Systems (1 mentions) Ratlaps eia kit, by Immunodiagnostic Systems (1 mentions) Leptin, by Tecan (1 mentions) Mbnmag 41k, by Merck Group (1 mentions) Fgf21, by Merck Group (1 mentions) Luminex 200 system, by Merck Group (1 mentions) Igf 1, by R&D Systems (1 mentions) Il 17a, by R&D Systems (1 mentions) Ifn γ, by R&D Systems (1 mentions) S monovette, by Sarstedt (1 mentions) Creatinine, by R&D Systems (1 mentions) Bs 120, by Mindray (1 mentions) Nad nadh ratio, by Merck Group (1 mentions) Ph meter, by Metrohm (1 mentions) Tumor necrosis factor (tnf), by R&D Systems (1 mentions) Interleukin 6 (il 6), by BD (1 mentions)

18 protocols using ctx 1

1

Serum Biomarkers of Bone Metabolism

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Serum PTH, Wnt10b and Procollagen 1 N-terminal Propeptide (P1NP) levels (n = 6 for each group) were measured in all experimental animals by using commercial enzyme-linked immunosorbent assay kits (PTH, Wnt10b and PINP are CUSABIO Biotech Co., Wuhan, China, and CTX-1 are Immunodiagnostic Systems, Boldon, United Kingdom.) according to the manufacturer’s guidance.
Zheng C.M., Hsu Y.H., Wu C.C., Lu C.L., Liu W.C., Zheng J.Q., Lin Y.F., Chiu H.W., Chang T.J., Shyu J.F, & Lu K.C. (2019). Osteoclast-Released Wnt-10b Underlies Cinacalcet Related Bone Improvement in Chronic Kidney Disease. International Journal of Molecular Sciences, 20(11), 2800.
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2

Serum Biomarkers of Bone Metabolism

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Serum levels of osteocalcin (Biomedical Technologies), TRAP-5b (Immunodiagnostic Systems), and CTX-1 (Immunodiagnostic Systems) were determined by ELISA.
Baum R., Sharma S., Organ J.M., Jakobs C., Hornung V., Burr D.B., Marshak-Rothstein A., Fitzgerald K.A, & Gravallese E.M. (2017). STING regulates abnormal bone formation induced by deficiency of DNase II. Arthritis & rheumatology (Hoboken, N.J.), 69(2), 460-471.
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3

Serum Biomarkers of Bone Metabolism in Mice

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Blood was collected from mice by cardiac puncture for analysis of several biochemical parameters. Serum total calcium was determined using Stanbio Laboratory Calcium Liquidcolor (CPC; Boerne, TX, USA; Catalog No. 0150‐250). Serum inorganic phosphate was determined using a Phosphate Colorimetric Assay Kit (BioVision, Inc., Milpitas, CA, USA; Catalog No. K410‐500). Serum C‐terminal telopeptides of types I and II collagen (CTX‐1 and CTX‐2; Immunodiagnostic Systems Inc., Fountain Hills, AZ, USA; Catalog No. AC‐06F1 and AC‐08F1, respectively), as well as N‐terminal propeptide of type I procollagen (P1NP) were determined using the RatLaps EIA kit (Immunodiagnostic Systems Inc., Fountain Hills, AZ, USA; Catalog No. AC‐33F1). Serum intact mouse parathyroid hormone (PTH(1‐84)) was measured using the mouse PTH(1‐84) EIA (Quidel Corp, San Diego, CA, USA; Catalog No. 60‐2305).
Reyes M., Firat D., Hanna P., Khan M., Bruce M., Shvedova M., Kobayashi T., Schipani E., Gardella T.J, & Jüppner H. (2023). Substantially Delayed Maturation of Growth Plate Chondrocytes in “Humanized” PTH1R Mice with the H223R Mutation of Jansen's Disease. JBMR Plus, 7(10), e10802.
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4

Serum Biomarkers of Bone Turnover

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Enzyme immunoassays were used to detect serum levels of procollagen type 1 amino‐terminal peptide (P1NP) and carboxyl‐terminal crosslinks peptide of type 1 collagen (CTX1) (Immunodiagnostic Systems) and osteoprotegerin (OPG) and RANKL (R&D Systems) according to the manufacturer's instructions.
Jaffery H., Huesa C., Chilaka S., Cole J., Doonan J., Akbar M., Dunning L., Tanner K.E., van ‘t Hof R.J., McInnes I.B., Carmody R.J, & Goodyear C.S. (2023). IĸB Protein BCL3 as a Controller of Osteogenesis and Bone Health. Arthritis & Rheumatology (Hoboken, N.j.), 75(12), 2148-2160.
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5

Serum Biomarkers in Animal Study

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The levels of serum osteocalcin (Immunodiagnostic Systems, Tyne and Wear, UK), carboxyl-terminal telopeptides of type 1 collagen (CTX-1) (Immunodiagnostic Systems, Tyne and Wear, UK), leptin (IBL International GmbH, Hamburg, Germany), adiponectin (Qayee Bio-Technology, Shanghai, China), and insulin (SPI-Bio, Bertin Pharma, Saclay, France) were measured using commercial rat-specific quantitative enzyme-linked immunosorbent assay kits, in accordance with the manufacturer’s instructions.
Wong S.K., Chin K.Y., Suhaimi F.H., Ahmad F, & Ima-Nirwana S. (2018). The Effects of Vitamin E from Elaeis guineensis (Oil Palm) in a Rat Model of Bone Loss Due to Metabolic Syndrome. International Journal of Environmental Research and Public Health, 15(9), 1828.
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6

Measuring Bone Turnover Markers

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Enzyme-linked immunosorbent assay (ELISA) kits were used to measure serum Tartrate-resistant acid phosphatase-5b (TRAcP-5b) (Kamiya Biochemical Company, Seattle, WA) and C-terminal cross-linked telopeptides of type I collagen (CTx-1) (Immunodiagnostic Systems, Fountain Hills, AZ) according to the manufacturer’s instructions.
Alikhani M., Alikhani M., Alansari S., Almansour A., Hamidaddin M.A., Khoo E., Lopez J.A., Nervina J.M., Nho J.Y., Oliveira S.M., Sangsuwon C, & Teixeira C.C. (2019). Therapeutic effect of localized vibration on alveolar bone of osteoporotic rats. PLoS ONE, 14(1), e0211004.
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7

Multiplex Analysis of Bone Biomarkers

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ELISA kits were used to detect the N-terminal propeptide of type 1 procollagen (P1NP), C-terminal telopeptide of type 1 collagen (CTX-1) (Immunodiagnostic Systems, Fountain Hills, AZ), receptor activator for nuclear factor κB ligand (RANKL), leptin, insulin-like growth factor-1 (IGF-1), adiponectin (R&D Systems, Minneapolis, MN, USA); and fibroblast growth factor-21 (FGF-21, Millipore Corp., Billerica, MA, USA). Multiplex analysis was conducted using a Luminex 200 system at the Human Immune Monitoring Core at Mount Sinai Icahn School of Medicine (New York, NY) using the metabolites for osteoprotegerin (OPG) and osteocalcin (OC, MBNMAG-41K, Millipore Corp.).
Ouattara A., Cooke D., Gopalakrishnan R., Huang T.H, & Ables G.P. (2016). Methionine restriction alters bone morphology and affects osteoblast differentiation. Bone Reports, 5, 33-42.
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8

Serum Biomarker Profiling in Mice

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Whole blood was collected via cardiac puncture at euthanasia; serum was isolated and stored at −80°C. Carboxy‐terminal collagen crosslinks type I collagen (CTX‐1; Immunodiagnostic Systems, East Boldon, UK), osteocalcin (OCN; Alfa Aesar, Haverhill, MA, USA), C3 (Abcam, Cambridge, UK), IL17A (R&D Systems), IGF‐1 (R&D Systems), and LCN2 (R&D Systems) were assessed by ELISA following the manufacturer's protocols.
Hathaway‐Schrader J.D., Poulides N.A., Carson M.D., Kirkpatrick J.E., Warner A.J., Swanson B.A., Taylor E.V., Chew M.E., Reddy S.V., Liu B., Westwater C, & Novince C.M. (2020). Specific Commensal Bacterium Critically Regulates Gut Microbiota Osteoimmunomodulatory Actions During Normal Postpubertal Skeletal Growth and Maturation. JBMR Plus, 4(3), e10338.
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9

Serum Biomarkers for Bone Metabolism

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Serum was separated by centrifugation and stored at −80 until analysis. Samples were assayed for total osteocalcin (Invitrogen, Waltham, MA cat# KAQ1381), and c-terminal telopeptide crosslinks (CTX-1) (Immunodiagnostic Systems, Tyne & Wear, UK, cat# AC-02F1) by enzyme-linked immunosorbent assay (ELISA). Serum biomarkers levels were interpolated from curves generated by manufacturer-provided osteocalcin or CTX-1 standards, respectively, included in the assay.
Watt J., Schuon J., Davis J., Ferguson T.F., Welsh D.A., Molina P.E, & Ronis M.J. (2019). Reduced serum osteocalcin in high-risk alcohol using people living with HIV (PLWH) does not correlate with systemic oxidative stress or inflammation: data from the New Orleans Alcohol Use in HIV (NOAH) study. Alcoholism, clinical and experimental research, 43(11), 2374-2383.
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10

Serum Biomarker Profiling Protocol

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Blood samples were put at room temperature to clot for 2 h before centrifuging for 10 min at 2000 g. The resultant sera were stored at −80 °C until analysis. The levels of PINP (BlueGene Biotech, Shanghai, China), CTX‐1 (Immunodiagnostic Systems, UK), IFN‐γ (R&D Systems, MN, USA), TNF‐α and IL‐2 (ABclonal Technology, Wuhan, China), RANKL and OPG (BlueGene Biotech, Shanghai, China) in serum or conditioned medium were detected according to the manufacturer's instructions.
Wang H., Lin S., Feng L., Huang B., Lu X., Yang Z., Jiang Z., Li Y., Zhang X., Wang M., Wang B., Kong L., Pan Q., Bai S., Li Y., Yang Y., Lee W.Y., Currie P.D., Lin C., Jiang Y., Chen J., Tortorella M.D., Li H, & Li G. (2023). Low‐Dose Staphylococcal Enterotoxin C2 Mutant Maintains Bone Homeostasis via Regulating Crosstalk between Bone Formation and Host T‐Cell Effector Immunity. Advanced Science, 10(28), 2300989.
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