Tubb β tubulin

Manufactured by Cell Signaling Technology

TUBB/β-tubulin is a protein that is a component of microtubules, which are part of the cytoskeleton in eukaryotic cells. TUBB/β-tubulin plays a critical role in the structure and function of microtubules, which are involved in various cellular processes such as cell division, intracellular transport, and cell motility.

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Lab products found in correlation

Horseradish peroxidase linked secondary antibody, by Cell Signaling Technology (1 mentions) Membrane integrity wb antibody cocktail, by Abcam (1 mentions) P crkl, by Cell Signaling Technology (1 mentions) Ab138500, by Abcam (1 mentions) P ulk1, by Cell Signaling Technology (1 mentions) Atg12, by Cell Signaling Technology (1 mentions) Flag epitope, by Merck Group (1 mentions) Torin2 sml1224, by Merck Group (1 mentions) Myc epitope, by Santa Cruz Biotechnology (1 mentions) Nef812001ea, by PerkinElmer (1 mentions) Nef822001ea, by PerkinElmer (1 mentions)

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β-tubulin (542 citations)

2 protocols using tubb β tubulin

Western Blotting of Autophagy Signaling

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Western blotting was performed using antibodies against p-CRKL (Cell Signaling Technology, 3181), LC3B (Cell Signaling Technology, 2775) TUBB/β-tubulin (Cell Signaling Technology, 2146), SQSTM1 (BD Biosciences, 610833), ATG7 (Epitomics, 2054–1) and Membrane Integrity WB Antibody Cocktail (Abcam, ab110414). Primary antibody detection was by enhanced chemiluminescence (GE Healthcare/Amersham, RPN2106) using a horseradish peroxidase-linked secondary antibody (Cell Signaling Technology, 7074 and 7076).

Karvela M., Baquero P., Kuntz E.M., Mukhopadhyay A., Mitchell R., Allan E.K., Chan E., Kranc K.R., Calabretta B., Salomoni P., Gottlieb E., Holyoake T.L, & Helgason G.V. (2016). ATG7 regulates energy metabolism, differentiation and survival of Philadelphia-chromosome-positive cells. Autophagy, 12(6), 936-948.

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Molecular Markers of Autophagy and Cell Signaling

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We used primary antibodies to the following proteins: LC3B (Cell Signaling Technology, 3868), ATG5 (Cell Signaling Technology, 12994), ATG7 (Cell Signaling Technology, 8558), ATG12 (Cell Signaling Technology, 4180), TUBB/β-Tubulin (Cell Signaling Technology, 2146), p-EIF4EBP1/4E-BP1 (Cell Signaling Technology, 2855), EIF4EBP1/4E-BP1 (Cell Signaling Technology, 9452), RPS6KB/S6K (Cell Signaling Technology, 2708), p-RPS6KB/S6K (Cell Signaling Technology, 9234), p-ULK1 (Ser317; Cell Signaling Technology, 12753), p-ULK1 (Ser757; Cell Signaling Technology, 14202), ULK1 (Cell Signaling Technology, 8054), mCherry (Medical & Biological Laboratories, PM005), GFP (Thermo Fisher Scientific, A11122), FLAG epitope (Sigma-Aldrich, F1804), MYC epitope (Santa Cruz Biotechnology, sc-789), BORCS5 (Abgent, AP5806b), SNAP29 (Abcam, ab138500) and VAMP8 (Abcam, ab76021). HRP-conjugated secondary antibodies were from Perkin Elmer (NEF822001EA, NEF812001EA). Secondary antibodies conjugated to Alexa Fluor dyes were from Thermo Fisher Scientific. Baf (B1793) and Torin2 (SML1224) were from Sigma-Aldrich.

Jia R., Guardia C.M., Pu J., Chen Y, & Bonifacino J.S. (2017). BORC coordinates encounter and fusion of lysosomes with autophagosomes. Autophagy, 13(10), 1648-1663.

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