Fsh elisa kit

Blood was collected from the eyes of the mice at approximately 2:00 P.M. For hormonal testing, whole blood was centrifuged at 1000 rpm for 15 min to extract plasma, which was then kept at −80 °C. The levels of serum testosterone, FSH, and LH from male mice were determined by Testosterone ELISA Kit (Arbor Assays, K032-H1), FSH ELISA Kit (Abbexa, abx154038), and LH (S-type) Rat ELISA KIT (FUJIFILM Wako, 630-23929), respectively. E2 ELISA kit (MYBioSource, MBS8800210), AHM ELISA kit (LSBio, LS-F6145-1), and FSH ELISA Kit (MYBioSource, MBS2700327) were employed to measure the levels of serum E2, AMH, and FSH from female mice, respectively. An ELISA microtiter plate reader (BioTek Synergy2) was utilized to determine the absorbance at 450 to 620 nm.

Fresh serum was used to detect hormone levels. Mouse luteinizing hormone (LH, ab235648) levels were measured using the Mouse LH Beta ELISA Kit (ab235648) provided by Abcam (Cambridge, UK). This kit is a sandwich ELISA designed for the quantitative measurement of LH protein in tissue extracts. The intensity was measured at 450 nm with a fluorescence microplate reader. A mouse follicle stimulating hormone (FSH) ELISA Kit (abx154039) and mouse free testosterone ELISA Kit (abx254089) were purchased from Abbexa (Cambridge, UK). Mouse LH, FSH and testosterone were detected using these kits according to the manufacturer’s instructions as previously described [44 (link), 45 (link)]. Serum samples and biotin-conjugated reagent were added to antibody-precoated wells. After that, the HRP-conjugated reagent and TMB substrate were then added. The intensity of the yellow colour was measured spectrophotometrically at 450 nm with a fluorescence microplate reader.