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Talon cellthru resin

Manufactured by Takara Bio
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TALON CellThru Resin is an affinity chromatography medium designed for the purification of His-tagged proteins. It features a proprietary resin that allows for the efficient capture and elution of target proteins while minimizing the co-elution of host cell proteins.

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Lab products found in correlation

Glutathione sepharose 4 fast flow medium, by GE Healthcare (1 mentions) Thrombin, by GE Healthcare (1 mentions)

Close spelling variants of this product

Talon CellThru Co2+-chelating resin Talon resin

4 protocols using talon cellthru resin

1

Recombinant Protein Expression and Purification

Cited 1 time
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The recombinant proteins were expressed in E. coli (BL21, DE3) as described previously [24 (link),30 (link)]. The hisx6-tagged trivalent vaccine and the dimeric P domain proteins were purified by TALON CellThru Resin (ClonTech) according to the instruction of the manufactory.
Xia M., Wei C., Wang L., Cao D., Meng X.J., Jiang X, & Tan M. (2016). A trivalent vaccine candidate against hepatitis E virus, norovirus, and astrovirus. Vaccine, 34(7), 905-913.
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2

Expression and Purification of mVP8* Antigens

Cited 4 times
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The hisx6-tagged S-mVP8* vaccines were expressed in E. coli (BL21, DE3) and purified by TALON CellThru Resin (ClonTech) according to the instruction of the manufacturer as described previously [17 , 30 (link), 31 (link)]. The GST-tagged mVP8* protein that was also expressed in same E. coli strain was purified using resin of Glutathione Sepharose 4 Fast Flow medium (GE Healthcare Life Sciences) according to the manufacturer’s instructions as described elsewhere [32 (link)-34 (link)].
Xia M., Huang P., Jiang X, & Tan M. (2019). Immune Response and Protective Efficacy of the S Particle Presented Rotavirus VP8* Vaccine in Mice. Vaccine, 37(30), 4103-4110.
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3

Recombinant Vaccine Expression in E. coli

Cited 1 time
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Recombinant vaccines were expressed in E. coli (BL21, DE3) as described previously37 (link)53 (link). The hisx6-tagged fusion protein and the individual P domains and VP8 proteins were purified by TALON CellThru Resin (ClonTech) according to manufacturer’s instruction.
Xia M., Wei C., Wang L., Cao D., Meng X.J., Jiang X, & Tan M. (2016). Development and evaluation of two subunit vaccine candidates containing antigens of hepatitis E virus, rotavirus, and astrovirus. Scientific Reports, 6, 25735.
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4

Purification of Recombinant Fusion Proteins

Cited 3 times
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The recombinant GST- and Hisx6-fused proteins were expressed in E. coli (BL21, DE3) as described previously.10 (link), 42 (link), 53 (link), 54 (link) The resulting recombinant proteins were purified using Sepharose 4 Fast Flow purification resin (GE Healthcare Life Sciences) for GST tagged and the TALON CellThru Resin (ClonTech) for the Hisx6-peptide fused proteins according to the instructions of the manufacturers. The GST was removed from the target proteins by thrombin (GE Healthcare Life Sciences) cleavage, while the GST-fusion proteins still bound to the purification resin.
Xia M., Huang P., Sun C., Han L., Vago F.S., Li K., Zhong W., Jiang W., Klassen J.S., Jiang X, & Tan M. (2018). Bioengineered Norovirus S60 Nanoparticles as a Multifunctional Vaccine Platform. ACS nano, 12(11), 10665-10682.
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