Rats in both groups (n = 6) were deeply anesthetized with sodium pentobarbital (200 mg/kg, i.p.) on PODs 7, 14, 21, and 28. Total RNA was extracted in TRIzol reagent (Invitrogen, TX, United States, Cat# 15596018). Subsequently, a High-Capacity cDNA Reverse Transcription Kit (Vazyme, Nanjing, China, Cat# R223) was used to convert RNA into cDNA, while a SYBR-Green Real-Time PCR Kit (Vazyme, Cat# Q311) and a Bio-Rad MiniOption thermocycler (Bio-Rad, CA, United States) were used for detection. Primer sequences are provided in Table 1 . The relative expression levels of RNA were evaluated using the 2−ΔΔCt method.
Minioption thermocycler
Manufactured by Bio-Rad
Sourced in United States, China
The Bio-Rad MiniOption thermocycler is a compact and efficient DNA amplification instrument. It is designed to perform polymerase chain reaction (PCR) for a wide range of applications in molecular biology and genetics research. The MiniOption provides precise temperature control and consistent thermal cycling to ensure reliable and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
High capacity cdna reverse transcription kit, by Vazyme (1 mentions)
Sybr green real time pcr kit, by Vazyme (1 mentions)
Sybr green 1 real time pcr kit, by Takara Bio (1 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using minioption thermocycler
1
Gene Expression Analysis in Rat Model
2
Quantifying Gene Expression via qRT-PCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The expression of PAR-1, SIRT1, and thrombin was measured by quantitative Real-Time PCR (qRT-PCR). TRIZOL reagent (Invitrogen, Grand Island, NY, USA) was applied to extract total RNA. Subsequently, the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems; Thermo Fisher Scientific, Inc.) was used to convert RNA into cDNA, and SYBR-Green I Real-Time PCR kit (TaKaRa Biotechnology, Dalian, China), with a Bio-Rad MiniOption thermocycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA), was used for detection. Primer sequences were as follows:
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