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Cd86 fitc rat

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CD86-FITC (rat) is a fluorescently labeled antibody that binds to the CD86 cell surface protein expressed on various cell types. It is used as a tool for the identification and analysis of CD86-positive cells in flow cytometry applications.

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Lab products found in correlation

Facsverse flow cytometer, by BD (2 mentions) Cd206 pe rat antibodies, by BD (2 mentions)

Spelling variants of this product

CD86-FITC (55 citations) FITC-conjugated rat anti-mouse CD86 (2 citations)

2 protocols using cd86 fitc rat

1

Microglial Phenotypic Characterization

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The microglial cells were collected and washed twice with PBS. The single cell suspensions were blocked by 0.1% Triton X-100 and 3% BSA in PBS, and then stained with CD86-FITC (rat) and CD206-PE (rat) antibodies (BD Biosciences, La Jolla, CA, USA) by the manufacturer's protocols, respectively. The microglial cells were examined with a BD FACSVerse flow cytometer (BD Biosciences), and all of the tests were controlled by the homologous isotype control antibodies.
Ji J., Wang J., Yang J., Wang X.P., Huang J.J., Xue T.F, & Sun X.L. (2019). The Intra-nuclear SphK2-S1P Axis Facilitates M1-to-M2 Shift of Microglia via Suppressing HDAC1-Mediated KLF4 Deacetylation. Frontiers in Immunology, 10, 1241.
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2

Phenotyping Microglia Activation States

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The microglia were collected and washed twice with PBS, and blocked by 0.1% Triton X‐100 and 3% BSA in PBS. The microglia were then stained with CD86‐FITC (rat) and CD206‐PE (rat) antibodies (BD Biosciences, La Jolla, CA, USA), respectively. The microglia were examined with a BD FACSVerse flow cytometer (BD Biosciences), and all of the tests were controlled by the homologous isotype control antibodies.
Ji J., Xue T., Guo X., Yang J., Guo R., Wang J., Huang J., Zhao X, & Sun X. (2018). Antagonizing peroxisome proliferator‐activated receptor γ facilitates M1‐to‐M2 shift of microglia by enhancing autophagy via the LKB1–AMPK signaling pathway. Aging Cell, 17(4), e12774.
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