Anti parp 1 sc 7150

DHA, DAPI, 4,5-dihydroxy-1,3-benzenedisulfonic acid (Tiron) and Oil red O were purchase from Sigma (St. Louis, MO, USA). The 2, 7-dichlorofluorescein diacetate (DCFH-DA) was purchased from Molecular Probes (Eugene, OR), and z-VAD-FMK was obtained from Calbiochem (La Jolla, CA, USA). The anti-caspase-8 antibody (9746) and the anti-CHOP/GADD153 (2859) antibodies were obtained from Cell signaling technology (Beverly, MA, USA). The anti-PARP-1 (sc-7150) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-KDEL antibody (ADI-SPA-827) was obtained from Enzo Life Sciences (Farmingdale, NY, USA). The anti-DR5 antibody (AB16942) was purchase from Millipore (Burlington, MA, USA).

Total cell lysate was prepared in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 2 mM sodium fluoride, 2 mM EDTA, 0.1% SDS and a mixture of protease inhibitors). Western blot analysis was performed using the same amount of protein. Protein concentration was determined by the Bradford (Sigma-Aldrich, St. Louis, MO, USA) method and equal quantities were subjected to Western blot analysis. SDS-PAGE-separated proteins were electroblotted onto a nitrocellulose membrane. The blots were incubated overnight at 4 °C with the following primary antibodies: anti-ERRα (ab76228; 1:1000); anti-SR-BI (ab52629; 1:1000); anti-HMGCR (ab215365; 1:500); from Abcam, Cambridge, UK; anti-Cyclin D1 (sc-8396; 1:500), anti-PARP-1 (sc-7150; 1:2000) and anti-GAPDH (sc-32233; 1:10,000) from Santa Cruz Biotechnology, Dallas, TX, USA. Anti βactin (ab8226; 1:1000; Abcam) was used as a loading control. All antibodies were incubated with appropriate horseradish peroxidase conjugated secondary antibodies for 1 h at room temperature. Immunoreactive bands were detected by the ECL Western blotting detection system (Santa Cruz Biotechnology, sc-2048). All the whole western blot figures can be found in the Supplementary Materials.