Whole blood from CLL patients (90 μl) was incubated with 10 μg/ml antibody for 1 hour at 37°C. 27 After this treatment, blood was stained with anti-CD3 FITC (UCHT1) and CD19 PE (HIB19) from BD Biosciences. Cal-lyse (Invitrogen) was used to simultaneously lyse RBCs while fixing the leukocytes. Count Bright absolute counting beads were added just prior to analysis by flow cytometry in order to calculate absolute concentrations of cells. To adjust for differences in initial leukemia counts between patients, data was normalized as follows: Percent depletion = 100 × (1 – [concentration of cells in treated sample] ÷ [concentration of cells in untreated control]). Additional details are provided in the supplemental materials.
Anti cd3 fitc ucht1
Manufactured by BD
Anti-CD3 FITC (UCHT1) is a fluorescently labeled monoclonal antibody that binds to the CD3 complex on the surface of T cells. It is commonly used in flow cytometry applications to identify and quantify T cell populations.
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Lab products found in correlation
2 protocols using anti cd3 fitc ucht1
1
Quantifying Leukocyte Depletion in CLL
2
Quantifying Leukocyte Depletion in CLL
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