NR8383 alveolar macrophages and the RAW 264.7 murine monocyte/macrophage cell line were obtained from the Chinese Academy of Sciences cell library (Shanghai, China) and cultured in Ham’s F-12K medium (J120703; Shanghai BasalMedia Technologies Co., Ltd. Shanghai, China). Cells were plated in 25-cm2 flasks and treated with or without one or more of the following: 50 μg/cm2 silica,22 (link) 30 ng/mL recombinant mouse RANKL (462-TEC-010; R&D Systems, Minneapolis, MN, USA),23 (link) 10 nmol/L Ac-SDKP (H-1164; Bachem AG, Torrance, CA, USA),18 (link) 10 nmol/L Ac-ADKP (substitution of Ser by Ala in Ac-SDKP) (PCM10745-0912; Pepmic, Suzhou, China),24 (link) and 10 μmol/L TLR-C34 (18512; Cayman Chemical, Ann Arbor, MI, USA).25 (link)
Ac sdkp h 1164
Manufactured by Bachem
Sourced in United States, Switzerland
Ac-SDKP (H-1164) is a synthetic tetrapeptide compound. It has the chemical formula C20H31N5O7. This product is intended for research purposes only and its core function is to serve as a chemical standard or reference material.
Automatically generated - may contain errors
2 protocols using ac sdkp h 1164
1
Alveolar Macrophage and Monocyte/Macrophage Responses to Silica and Signaling Pathways
2
Rat Lung Fibroblast Angiotensin II Responses
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Rat lung fibroblasts were isolated from 3-week-old Wistar rats using a trypsin digestion method and were maintained in complete culture medium [DMEM (BI-SH0019, BI, Kibbutz Beit-Haemek, Israel) supplemented with 10% fetal bovine serum (FBS, 10099141, Gibco, Thermo Fisher Scientific) and 100 U/ml penicillin/streptomycin] in a 37 °C incubator with 5% CO2. Cells at 80% confluence were cultured in FBS-free MEM medium for 24 hours, when most cells were in quiescent state. Next, the cells were divided into 6 groups and were cultured for 24 hours: 1) control; 2) 100 nmol/L Ang II (A9525, Sigma-Aldrich); 3) Ang II + 10−8 mol/L Ac-SDKP (H-1164, Bachem AG, Switzerland); 4) Ang II + 10−6 mol/L valsartan (V0112, Tokyo Chemical Industry, Tokyo, Japan); 5) Ang II + 10−6 mol/L TCS HDAC6 20b (4805, Tocris Bioscience, Bristol, United Kingdom); 6) Ang II + 10−3 mol/L Y-27632 (10005583 m, Cayman Chemical Company, Ann Arbor, MI, USA).
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