A single colony of P. agglomerans KM1 was grown in LB broth (Conda, Spain) overnight at 37°C. The genomic DNA was isolated using the LaboPass™ Tissue Genomic DNA mini kit (Cosmo Genetech, Seoul, South Korea) according to the manufacturer’s protocol. The identity of the isolate was verified through 16S rRNA gene sequencing. The 16S rRNA gene was amplified from the extracted genomic DNA using 16S universal primers 27F (5’-AGA GTTTGATCMTGGCTCAG-3’ ) and 1492R (5’-GGTTACCTTGTTACGACTTC-3’ ) and sequenced using an automated ABI3730XL capillary DNA sequencer (Applied Biosystems, USA) for taxonomic identification at Cosmo Genetech (Seoul, South Korea). The 16S rRNA sequences were confirmed through BLASTn search against the NCBI microbial 16S database.
Labopass tissue genomic dna mini kit
Manufactured by Cosmogenetech
Sourced in Cameroon
The LaboPass™ Tissue Genomic DNA mini kit is a laboratory equipment designed for the extraction and purification of genomic DNA from various tissue samples. It provides a simple and efficient method to obtain high-quality DNA for further applications.
Automatically generated - may contain errors
2 protocols using labopass tissue genomic dna mini kit
1
Identification of P. agglomerans KM1 by 16S rRNA sequencing
2
Identification of P. agglomerans KM1 by 16S rRNA
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A single colony of P. agglomerans KM1 was grown in LB broth (Conda, Spain) overnight at 37 o C. The genomic DNA was isolated using the LaboPass™ Tissue Genomic DNA mini kit (Cosmo Genetech, Seoul, South Korea) according to the manufacturer's protocol. The identity of the isolate was verified through 16S rRNA gene sequencing. The 16S rRNA gene was amplified from the extracted genomic DNA using 16S universal primers 27F (5'-AGA GTTTGATCMTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTTC-3') and sequenced using an automated ABI3730XL capillary DNA sequencer (Applied Biosystems, USA) for taxonomic identification at Cosmo Genetech (Seoul, South Korea). The 16S rRNA sequences were confirmed through BLASTn search against the NCBI microbial 16S database.
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