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Human igg1

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Human IgG1 is a purified immunoglobulin G (IgG) subclass 1 protein derived from human serum. IgG1 is the most abundant IgG subclass in human serum and plays a crucial role in the adaptive immune response.

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Lab products found in correlation

Nod scid gamma (nsg), by Jackson ImmunoResearch (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Rankl, by Thermo Fisher Scientific (1 mentions) Dulbecco phosphate buffered saline (dpbs), by Merck Group (1 mentions) β glycerol phosphate, by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Human macrophage colony stimulating factor m csf, by Thermo Fisher Scientific (1 mentions) Human igg4, by BioLegend (1 mentions) Aducanumab, by Creative Biolabs (1 mentions) Murine igg1, by BioLegend (1 mentions)

Spelling variants of this product

Human IgG1 isotype control antibody (4 citations) Ultra-LEAF purified human IgG1 Isotype control (3 citations) Ultra-LEAF Purified Human IgG1 isotype control antibody (2 citations) Anti-human igG1 (2 citations) Anti-human IgG1 FITC (2 citations) Recombinant mouse PD-L1-Ig chimera or human IgG1-Ig (2 citations) Anti-human CD166 mouse IgG1 monoclonal antibody clone 3A6 (2 citations) Human IgG1 isotype control (2 citations)

4 protocols using human igg1

1

Humanized Mouse Model for GVHD

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Human neonatal foreskins were grafted onto the backs of 6- to 8-week old nonobese diabetic /severe combined immunodeficient/IL-2 receptor γ chain null mice (NSG, Jackson Laboratory, Bar Harbor, ME). One week later, 3 × 106 allogeneic PBMCs, TCM, TMM or TEM were injected intravenously. In indicated experiments, alemtuzumab (2.5 μg) or isotype control (human IgG1, BioLegend) was injected i.p. three times per week for 3 weeks. Three weeks after cell injection, blood, spleen and skin grafts were harvested for analysis. All experiments were approved by the Brigham and Women’s Hospital Institutional Animal Care and Use Committee (IACUC protocol # 2016N000591).
Matos T.R., Gehad A., Teague J.E., Dyring-Andersen B., Benezeder T., Dowlatshahi M., Crouch J., Watanabe Y., O’Malley J.T., Kupper T.S., Yang C., Watanabe R, & Clark R.A. (2022). Central memory T cells are the most effective precursors of resident memory T cells in human skin. Science immunology, 7(70), eabn1889.
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2

Humanized Mouse Model of Skin Graft Rejection

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Human neonatal foreskins were grafted onto the backs of 6- to 8-week-old NOD/SCID/IL-2 receptor γ chainnull mice (Jackson Laboratories). One week later 5 x 106 allogeneic PBMC were injected i.v. after isolation from healthy donors by ficoll centrifugation. Antibody injections commenced 2–4 weeks after PBMC engraftment. Alemtuzumab (2.5 μg) or isotype control (human IgG1, Biolegend) was injected i.p. 3 times/week for 3 weeks. Anti-CD3-diphtheria toxin (A-dmDT(390)-bisFv(UCHT1), 0.625 μg) or isotype control (human IgG1) was injected i.v. once/day for 4 days. At the conclusion of the experiment, blood and skin grafts were harvested for analysis by flow cytometry, immunofluorescence and/or hematoxylin/eosin staining.
Watanabe R., Gehad A., Yang C., Campbell L., Teague J.E., Schlapbach C., Elco C., Huang V., Matos T.R., Kupper T.S, & Clark R.A. (2015). Human skin is protected by four functionally and phenotypically discrete populations of resident and recirculating memory T cells. Science translational medicine, 7(279), 279ra39.
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3

In vitro Immune Checkpoint Inhibitor Assay

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Cell culture medium α-Modified Essential Medium+GlutaMAX (α-MEM), L-glutamine, and fetal bovine serum (FBS) were purchased from Gibco, Life Technologies Limited, UK. Dulbecco phosphate-buffered saline and media supplements (including penicillin/streptomycin, β-glycerol phosphate, ascorbic acid, and dexamethasone) were purchased from Sigma Aldrich, USA. The cytokines human macrophage colony-stimulating factor (M-CSF) and human receptor activator of nuclear factor kappa B ligand (RANKL) were obtained from Peprotech, UK. The PD1i pembrolizumab and the PD-L1i atezolizumab were purchased from Selleck Chemicals, TX, USA; as an antibody control, we used the human IgG1 from Biolegend, San Diego, CA, USA (cat. number 403501). In line with most in vitro studies,10 11 (link) experiments were performed with ICI concentrations of 0.05–1 µg/mL, which reflect the poor biodistribution of ICIs in the bone.12–14 (link)
Gassner T., Chittilappilly C., Pirich T., Neuditschko B., Hackner K., Lind J., Aksoy O., Graichen U., Klee S., Herzog F., Wiesner C., Errhalt P., Pecherstorfer M., Podar K, & Vallet S. (2024). Favorable impact of PD1/PD-L1 antagonists on bone remodeling: an exploratory prospective clinical study and ex vivo validation. Journal for Immunotherapy of Cancer, 12(5), e008669.
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4

Murine and Humanized PMN310 Antibody Production

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The murine PMN310 (muPMN310) monoclonal antibody used in the studies presented was either purified from hybridoma supernatant by ImmunoPrecise Antibodies or produced by WuXi Biologics (Hong Kong, China) by transient transfection of Chinese hamster ovary (CHO) cells with a vector encoding the hybridoma variable regions cloned into a murine IgG1 framework. Humanized PMN310 (huPMN310) was generated by Abzena (Cambridge, UK) by cloning the humanized variable region genes of muPMN310 into vectors encoding a human IgG4 (S241P hinge variant) heavy chain constant domain and a human kappa light chain constant domain. Aducanumab and bapineuzumab were purchased from Creative Biolabs (Upton NY, USA). Negative isotype controls including murine IgG1, human IgG1 and human IgG4 were purchased from BioLegend (San Diego CA, USA).
Gibbs E., Silverman J.M., Zhao B., Peng X., Wang J., Wellington C.L., Mackenzie I.R., Plotkin S.S., Kaplan J.M, & Cashman N.R. (2019). A Rationally Designed Humanized Antibody Selective for Amyloid Beta Oligomers in Alzheimer’s Disease. Scientific Reports, 9, 9870.
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