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9 protocols using tetrahydrofuran

1

Bioactive Curcumin-Loaded Polymer Scaffolds

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Poly(ε-caprolactone) (PCL molecular weight of 80,000 Da) was purchased from Sigma Aldrich while poly (l-lactide-co-d,l-lactide) (PLA 4032 D-Mw = 160,000 g/mol) was purchased from NatureWorks (Minnetonka, MN, USA). Tetrahydrofuran (THF pure-CAS: 109-99-9), Ethanol (EtOH purity > 96%-CAS 64-17-5) and Phosphate Buffer Solution (PBS-pH = 7 ± 0.02-CAS: 7558-79-4) were purchased from Carlo Erba Reagents (Cornaredo-Milano). N,N-Dimethylformamide (DMF-CAS 68-12-2) and Curcumin (Cur) were purchased from Sigma Aldrich (Milan, Italy). Cell Counting Kit-8 (CCK-8) assay and Lactate dehydrogenase assay were from Roche Applied Science (Milan, Italy). Human dermal fibroblasts were purchased from the American Type Culture Collection ATCC (LGC Standards S.R.L., Sesto San Giovanni, Milan, Italy) and cultured in accordance with the manufacturer’s instructions. Fetal bovine serum (FBS), Dulbecco’s Modified Eagle’s Medium (DMEM), sodium pyruvate, L-glutamine, penicillin, and streptomycin were purchased from Hyclone (Milan, Italy).
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2

PLGA-based Nanoparticle Synthesis Protocol

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Poly (D,L-lactide-co-glycolide) (PLGA) (50:50 Resomer RG 502H inherent viscosity 0.16–0.24 dL/g) was purchased from Boehringer Ingelheim (Ingelheim, Germany). Polyethyleneimine (PEI, MW = 10–25 kDa branched), poloxamer 188 (Pluronic® F68), Bradford reagent, potassium phosphate dibasic and potassium phosphate monobasic, sodium azide, sodium chloride, copper (II) sulphate and albumin from chicken egg white (OVA), were purchased from Sigma-Aldrich. Sodium hydroxide was provided from Delchimica Scientific Glassware. Ethanol (96 %), phosphoric acid (85 %), acetic acid, acetonitrile and tetrahydrofuran were purchased from Carlo Erba Reagenti (Milan, Italy).
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3

Fatty Acid and Compound Extraction

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Butanol, chloroform, isopropanol, methanol, n-hexane and tetrahydrofuran (THF) were purchased from Carlo Erba Reagents (Milan, Italy). Alpha-linolenic acid (LNA), docosahexaenoic acid (DHA), Resveratrol, stearic acid, polyoxyethylene (20) sorbitan monooleate (Tween-20), taurodeoxycolic acid, 4-dimethylaminopyridine (DMAP), dicyclohexylcarbodiimide DCC), sodium taurocholate hydrate, trichloroacetic acid (TCA), thiobarbituric acid (TBA), butylated hydroxytoluene (BHT), deuterated chloroform (CDCl3) were purchased from Sigma-Aldrich (Sigma Chemical Co., St. Louis, MO, USA).
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4

Curcumin-Loaded Polymeric Micelles

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Poly(ε-caprolactone) (PCL) in pellet form (Mw = 80,000 Da; CAS: 24980-41-4) and Poly[(R)-3-hydroxybutyric acid] (CAS: 29435-48-1) were purchased from Sigma Aldrich (Milan, Italy). Tetrahydrofuran (THF pure, CAS: 109-99-9), Ethanol (EtOH purity >96%-CAS: 64-17-5) and Phosfate Buffer Solution (PBS) (pH = 7 ± 0.02, CAS:7558-79-4) were purchased from Carlo Erba Reagents (Cornaredo-Milano). N,N-Dimethylformamide (DMF; CAS: 68-12-2) and Chloroform (CHCl3; CAS: 67-66-3) were purchased from Sigma Aldrich. Curcumin from Curcuma Longa (Curc) (Mw = 368.38 g/mol; CAS: 458-37-7) was purchased in powder form from Sigma-Aldrich.
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5

Synthesis and Antioxidant Evaluation of Gallic Acid and Phloretin Derivatives

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Acetone, hydrochloric acid, chloroform, diethyl ether, ethanol, isopropanol, methanol, n-hexane, tetrahydrofuran (THF), allyl alcohol, and sodium sulfate were purchased from Carlo Erba Reagents (Milan, Italy). Gallic acid (MW = 170.12), phloretin (MW = 274.27), dicyclohexylcarbodiimide (DCC), N,N-dimethylaminopyridine (DMAP), potassium tert-butoxide, N,N-dimethylacrylamide (DMAA), ammonium persulfate (NH4)2S2O8, N,N′-methylene-bis-acrylamide, sorbitan trioleate (Span85), polyoxymethylene sorbitan trioleate (Tween85), N,N,N′,N′-tetramethyl-ethylenediamine (TMEDA), tert-butylhydroperoxide (t-BOOH), trichloroacetic acid (TCA), 2-thiobarbituric acid (TBA), and butylated hydroxytoluene (BHT) were Purchased from Sigma-Aldrich (Sigma Chemical Co, St. Louis, MO, USA).
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6

Supramolecular Extraction of Aluminum

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Analytical grade chemicals including 1-hexanol (≥ 99%, d = 0.814 g mL−1), 1-dodecanol (≥ 98.0%, d = 0.83 g cm−3), 1-decanol (≥ 99.0%, d = 0.80 g cm−3), 1-tetradecanol (≥ 97%, d = 0.823 g cm−1, Sigma), and tetrahydrofuran (Carlo Erba, THF) were used to prepare alkanol-based SUPRASs. Ultrapure water was prepared by Milli-Q water purification system (Millipore, Bedford, MA, USA). Stock solution (1000 mg L−1) of Al(III) ion was prepared from Al(NO3)3 salt (Sigma, St Louis, MO, USA) in water and stored at 4 °C in a refrigerator. A stock solution of morinhydrate was prepared in ethanol at a concentration of 1000 mg L−1 (Carlo Erba, Milan, Italy) and stored in dark. The calibration solutions were freshly prepared by sequential dilution of the stock solutions. A 5% (w/v) of KCl solution was prepared by dissolving 5.0 g of KCl (Sigma) in 100 mL deionized water. Borate, acetate, phthalate, and citrate buffer solutions were used to adjust solutions pH. To minimize all possible contamination of glassware, a diluted HNO3 solution was used for washing glassware and finally rinsed with distilled water.
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7

Extraction and Analysis of Bioactive Compounds

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Potassium iodide, glacial acetic acid, chloroform, sodium thiosulfate, sodium hydroxide, starch, β-carotene, α-tocotrienol, α-tocopherol, and α-cholestanol were purchased from Sigma Aldrich (Milan, Italy). Petroleum ether, diethyl ether, ethanol, tetrahydrofuran, n-hexane, citric acid, and sodium sulfate anhydrous were purchased from Carlo Erba (Rodano, Italy). Bleaching earth was purchased from Clariant (Milan, Italy).
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8

Synthesis and Characterization of Polyhydroxyalkanoate with Unsaturated Sidechains

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Poly(3-hydroxyoctanoate-co-3-hydroxyundecenoate) with 33% double bonds PHOU was provided from the Swiss Federal Laboratory for Materials Testing and Research (EMPA, Switzerland, Mn = 40,000 g/mol, Polydispersity index 1.7). The different comonomers are randomly distributed. CaCl2, BaCl2, MgCl2, and Sodium 2-mercapto-1-ethanesulfonate (98%) were purchased from Aldrich. Tannic acid was purchased from Alfa Aesar. Tetrahydrofuran (THF), dimethylsulfoxide (DMSO), methanol, and ethanol absolute anhydrous were purchased from Carlo Erba. Gallic acid, 2,2-dimethoxy-2-phenylacetophenone (DMPA, Irgacure 651), glycidyltrimethyl ammonium (GTMA), triphenylphosphine (TPP), and methyl hydroxyquinone (MEHQ), were supplied from Sigma-Aldrich and were used without prior purification. Butylacetate was supplied from Merck. Tannic acid was purchased from Alfa Aesar.
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9

Antioxidant Capacity Assay Protocol

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Hydrochloric acid, chloroform, diethyl ether, ethanol, isopropanol, methanol, n-hexane, tetrahydrofuran (THF) and sodium sulfate were purchased from Carlo Erba Reagents (Milan, Italy). Gallic acid (MW = 170.12), phloretin (MW = 274.27), acrylic acid (MW = 72.06, d = 1051 g/mL), dicyclohexylcarbodiimide (DCC), N,N-dimethylaminopyridine (DMAP), N, N-dimethylacrylamide (DMAA), tert-butylhydroperoxide (t-BOOH), trichloroacetic acid (TCA) acid, 2-thiobarbituric acid (TBA), and butylated hydroxytoluene (BHT) were purchased from Sigma-Aldrich (SGFma Chemical Co., St Louis, MO, USA). Folin–Ciocolteau and 2,2-difenil-1-picrylidrazyl (DPPH) were purchased from Sigma-Aldrich (SGFma Chemical Co., St Louis, MO, USA), while acid 2,2′-azino-bis (ABTS) was purchased from Alfa Aesar (Ward Hill, MA, USA).
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