Reagents and solvents were purchased from Sigma Aldrich (Taufkirchen, Germany), Glen Research (Sterling, VA, USA), or Alfa Aesar (Thermo Fisher Scientific, Waltham, MA, USA) and were used without further purification. Phospholipids were purchased from Bachem AG (Bubendorf, Switzerland) or Avanti Polar Lipids (Alabaster, AL, USA).
Fluorescent dyes were purchased from Sigma Aldrich (Germany). Cell culture media and additives were from Gibco
® (Thermo Fisher Scientific, Waltham, MA, USA).
For the mass spectrometry analysis, an
Ultraflex III MALDI-TOF-TOF instrument (Bruker Daltonics GmbH, Bremen, Germany) equipped with a Smartbeam 2 laser with a repetition rate up to 200 Hz (500 laser shots) was used. The obtained mass spectrum was analyzed with Flex-analysis version 4.0 software (Bruker Daltonics GmbH).
The HPLC separation and analysis were performed with Waters
® Alliance 2695 (Waters
® Corporation, Milford, MA, USA) apparatus equipped with the ultraviolet (UV) detector Waters
® 486 (Waters Corporation) and analyzed with
Millennium 32 software (Waters Corporation). The reverse phase analytical
C8 column (Macherey-Nagel, GmbH & Co, KG, Hoerdt, France) was used for all separations.
Beztsinna N., Tsvetkova Y., Jose J., Rhourri-Frih B., Al Rawashdeh W., Lammers T., Kiessling F, & Bestel I. (2017). Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers. International Journal of Nanomedicine, 12, 3813-3825.