Protein samples (30–50 μ g lysate; total bound) were resolved on Criterion 4–20% gradient gels (Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad) and Myc-tagged proteins were detected with anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling), TAP-tagged proteins were detected with peroxidase anti-peroxidase antibody (1:5000; Sigma), Nrd1 was detected with anti-Nrd1 rabbit polyclonal antibody (1:3,000; a gift from David Brow), Nab3 was detected with anti-Nab3 monoclonal antibody (1:2000; a gift from Maurice Swanson), GFP was detected with anti-GFP rabbit polyclonal antibody (1:3000; Sigma), His-tagged Rrp6 was detected with anti-His monoclonal antibody coupled to horseradish peroxidase (1:2000; Invitrogen), and GST fusion proteins were detected with anti-GST monoclonal antibody (1:2000; Santa Cruz Biotechnology).
Anti gfp rabbit polyclonal antibody
Manufactured by Merck Group
The Anti-GFP rabbit polyclonal antibody is a laboratory reagent used for the detection and analysis of green fluorescent protein (GFP) in various experimental systems. This antibody is produced in rabbits and is designed to specifically bind to GFP, enabling researchers to identify and quantify GFP-tagged proteins or cells.
Automatically generated - may contain errors
2 protocols using anti gfp rabbit polyclonal antibody
1
Western Blot Analysis of Protein Samples
2
Western Blot Protein Detection
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Protein samples (30‐50 μg lysate; total bound) were resolved on Criterion 4% ‐20% gradient gels (Bio‐Rad), transferred to nitrocellulose membranes (Bio‐Rad) and Myc‐tagged proteins were detected with anti‐Myc monoclonal antibody 9B11 (1:2000; Cell Signaling), GFP‐tagged proteins were detected with anti‐GFP rabbit polyclonal antibody (1:3000; Sigma), and GST fusion proteins were detected with anti‐GST monoclonal antibody (1:2000; Santa Cruz Biotechnology).
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